Detailed information NTG0148674

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AB688_RS06755
• Genome accession: NZ_CP011789
• Coordinates: 1482822..1483688 (-)
• Length: 288 a.a.
• NCBI ID: WP_063542963.1
• Uniprot ID: -
• Organism: Pseudomonas putida strain PC2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1477822..1488688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB688_RS06720 (AB688_06730)	phnN	1478119..1478679 (+)	561	WP_063542955.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
AB688_RS06725 (AB688_06735)	-	1478785..1480080 (-)	1296	WP_054895236.1	NAD(P)/FAD-dependent oxidoreductase	-
AB688_RS06730 (AB688_06740)	-	1480165..1480338 (-)	174	WP_063542957.1	DUF3094 family protein	-
AB688_RS06735 (AB688_06745)	-	1480482..1481111 (-)	630	WP_054895238.1	DUF1780 domain-containing protein	-
AB688_RS06740 (AB688_06750)	-	1481224..1481919 (+)	696	WP_063542959.1	energy-coupling factor ABC transporter permease	-
AB688_RS06745 (AB688_06755)	yacG	1482002..1482202 (-)	201	WP_054895240.1	DNA gyrase inhibitor YacG	-
AB688_RS06750 (AB688_06760)	coaE	1482199..1482822 (-)	624	WP_063542961.1	dephospho-CoA kinase	-
AB688_RS06755 (AB688_06765)	pilD	1482822..1483688 (-)	867	WP_063542963.1	prepilin peptidase	Machinery gene
AB688_RS06760 (AB688_06770)	-	1483690..1484898 (-)	1209	WP_063542965.1	type II secretion system F family protein	-
AB688_RS06765 (AB688_06775)	-	1485112..1485552 (+)	441	WP_063542967.1	pilin	-
AB688_RS06775 (AB688_06785)	rclC	1486225..1486824 (-)	600	WP_063542969.1	reactive chlorine resistance membrane protein RclC	-
AB688_RS06780 (AB688_06790)	-	1486997..1487944 (+)	948	WP_063542971.1	AraC family transcriptional regulator	-
AB688_RS06785 (AB688_06795)	-	1487995..1488255 (-)	261	WP_193217512.1	DUF2790 domain-containing protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31614.61 Da
• Isoelectric Point: 7.2856

>NTDB_id=148674 AB688_RS06755 WP_063542963.1 1482822..1483688(-) (pilD) [Pseudomonas putida strain PC2]
MNLWTFLVDQPGFFLASAAVLGLLVGSFLNVLVHRLPVMLERQWQNEAREVLGMPVEAHERFDLCLPASHCPGCGHRIRA
WENIPVVSYLALRGRCSACKGRISLRYPLVELACAGLSLVVAWHFGVSGQALLALVLTWCLLALSLIDAEHQLLPDVLVL
PTLWLGLVVNAFGVFVPLADALWGAVVGYLSLWSVYWLFRLVTGKEGLGYGDFKLLALLGAWGGWQVLPLTLLLSSVVGA
VIGLCLLRLRKASLGTTMPFGPYLAIAGWIAWLWGDEIYASYMQLLGY

Nucleotide

• Length: 867 bp

>NTDB_id=148674 AB688_RS06755 WP_063542963.1 1482822..1483688(-) (pilD) [Pseudomonas putida strain PC2]
ATGAATCTGTGGACTTTTCTTGTCGACCAGCCAGGGTTCTTCCTGGCGAGTGCAGCCGTGCTCGGGCTGCTGGTGGGCAG
TTTTCTGAATGTGCTGGTGCATCGGTTGCCGGTGATGCTCGAACGCCAGTGGCAGAACGAGGCGCGTGAGGTGCTGGGGA
TGCCGGTGGAGGCGCATGAGCGTTTCGACCTGTGCCTGCCGGCCTCGCACTGCCCGGGCTGCGGGCATCGGATTCGGGCG
TGGGAAAACATTCCGGTGGTGAGTTACCTGGCCCTGCGTGGGCGCTGCTCGGCGTGCAAGGGGCGGATCAGCCTGCGTTA
TCCGCTGGTGGAGCTGGCCTGCGCGGGGCTGTCGCTGGTGGTGGCCTGGCATTTCGGGGTGAGCGGGCAGGCGTTGTTGG
CGCTGGTGCTGACGTGGTGCCTGCTGGCCCTGAGCCTGATCGATGCGGAGCATCAATTGCTGCCGGATGTGCTGGTGCTG
CCGACGCTGTGGCTGGGGCTGGTGGTCAATGCCTTCGGGGTGTTCGTGCCGCTGGCCGATGCGCTGTGGGGGGCGGTGGT
CGGTTACCTGAGCCTATGGTCGGTGTATTGGCTGTTTCGGCTGGTCACCGGCAAGGAGGGCCTGGGCTATGGCGATTTCA
AGCTGCTGGCGCTGCTGGGCGCCTGGGGCGGCTGGCAGGTGCTGCCGCTGACACTGTTGCTGTCGTCGGTGGTGGGGGCG
GTGATCGGGCTGTGCCTGCTGCGGCTGCGCAAGGCTTCGCTGGGGACGACGATGCCTTTTGGGCCCTATCTGGCAATTGC
GGGCTGGATCGCATGGCTCTGGGGTGATGAAATATACGCTTCCTACATGCAACTGCTCGGATACTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.33	96.875	0.507
pilD	Acinetobacter baumannii D1279779	51.301	93.403	0.479
pilD	Vibrio campbellii strain DS40M4	50.185	94.097	0.472
pilD	Acinetobacter nosocomialis M2	50.558	93.403	0.472
pilD	Neisseria gonorrhoeae MS11	48.327	93.403	0.451