Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   L63ED372_RS00345 Genome accession   NZ_CP011774
Coordinates   79501..81042 (-) Length   513 a.a.
NCBI ID   WP_062401704.1    Uniprot ID   A0A0P0M6N7
Organism   Limnohabitans sp. 63ED37-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 74501..86042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L63ED372_RS00320 (L63ED372_00064) - 74819..75829 (+) 1011 WP_062401691.1 tripartite tricarboxylate transporter substrate binding protein -
  L63ED372_RS00325 (L63ED372_00065) - 75829..76812 (+) 984 WP_062401694.1 alpha/beta hydrolase -
  L63ED372_RS00330 (L63ED372_00066) - 76896..77669 (-) 774 WP_062401697.1 YecA family protein -
  L63ED372_RS00335 (L63ED372_00067) - 77689..78171 (-) 483 WP_062401700.1 PIN domain-containing protein -
  L63ED372_RS00340 (L63ED372_00068) - 78168..79487 (-) 1320 WP_062401702.1 THUMP domain-containing protein -
  L63ED372_RS00345 (L63ED372_00069) rpoN/rpoN1 79501..81042 (-) 1542 WP_062401704.1 RNA polymerase factor sigma-54 Machinery gene
  L63ED372_RS00350 (L63ED372_00070) lptB 81080..81838 (-) 759 WP_062401706.1 LPS export ABC transporter ATP-binding protein -
  L63ED372_RS00355 (L63ED372_00071) lptA 81835..82464 (-) 630 WP_197275288.1 lipopolysaccharide transport periplasmic protein LptA -
  L63ED372_RS00360 (L63ED372_00072) - 82617..83258 (-) 642 WP_062401711.1 thiol:disulfide interchange protein DsbA/DsbL -
  L63ED372_RS00365 (L63ED372_00073) - 83305..83937 (-) 633 WP_062401713.1 SPOR domain-containing protein -
  L63ED372_RS00370 (L63ED372_00074) argS 83949..85658 (-) 1710 WP_062401716.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 513 a.a.        Molecular weight: 56320.56 Da        Isoelectric Point: 4.6269

>NTDB_id=148595 L63ED372_RS00345 WP_062401704.1 79501..81042(-) (rpoN/rpoN1) [Limnohabitans sp. 63ED37-2]
MKPGLSLRMSQHLALTPQLQQSIRLLQLSTLELSQEVDQMLDENPFLERAEEEAPAEAFGLDQADTHVSLGDRVSESAST
TSSSDDGPSETRDEAVTDVAESWDGDGSVDMRPDDSEWGGDAAPRQNNGEETADAIDLARSSGSLTDFLHRQALSLRLSE
VDSAALRFLIESLNDDGYLTDTLPELATSLAGSDDYEQIDELVHRFTVALGLLQSLEPVGVGARDLAECLRIQIKAWLQD
DPDDEVAQAALAMCAQPLEMLAKRDFKRLAVACGLNEAVVKTAIAFIARLEPKPGRPFVQAEQNIIIPDVLVVNTSKTAT
PKFRIQLNPDVMPKLRVHDIYANALRGGKGDNHAGLQQRLQEARWFIKNIQQRFDTILRVSTAIVERQKNFLTHGELAMR
PLVLREIADELGMHESTISRVTTAKYMATPQGTFELKYFFGSGLGTDSGSAASSTAVRALIKQFVAAEKPAKPLSDNQIS
EMLKEQGIDCARRTVAKYREALKIAPANLRKTL

Nucleotide


Download         Length: 1542 bp        

>NTDB_id=148595 L63ED372_RS00345 WP_062401704.1 79501..81042(-) (rpoN/rpoN1) [Limnohabitans sp. 63ED37-2]
ATGAAGCCCGGCCTGTCGCTTCGCATGTCCCAGCACCTGGCGCTCACGCCCCAGTTGCAACAATCGATTCGCCTGCTGCA
ACTGTCCACGCTGGAGTTGTCGCAAGAGGTCGACCAAATGCTGGACGAGAACCCGTTTTTGGAGCGTGCCGAAGAAGAAG
CGCCCGCCGAGGCCTTTGGCCTGGACCAAGCCGACACCCACGTCAGCCTGGGCGACCGGGTGAGCGAAAGCGCCAGCACC
ACCAGCAGCAGCGACGATGGCCCCAGCGAAACCCGTGACGAAGCGGTGACCGATGTGGCCGAGAGCTGGGACGGTGACGG
GAGCGTGGACATGCGCCCCGACGACAGCGAATGGGGCGGTGACGCGGCACCACGCCAAAACAACGGCGAAGAAACCGCCG
ACGCCATTGACCTGGCCCGCAGCAGTGGGTCCCTCACTGACTTTTTACACCGCCAGGCGCTGTCGCTGCGCCTGTCCGAA
GTGGACAGCGCTGCGCTGCGCTTTCTGATCGAGTCGCTCAACGACGACGGCTACCTGACCGACACCCTGCCCGAGCTGGC
CACGAGCTTGGCGGGCAGCGATGATTACGAGCAAATCGACGAGCTGGTGCACCGATTCACGGTGGCGCTGGGTTTGCTGC
AATCGCTCGAACCCGTGGGCGTGGGCGCACGCGACTTGGCCGAGTGCCTGCGCATCCAAATCAAGGCCTGGCTGCAGGAC
GACCCCGACGACGAAGTGGCCCAAGCCGCGCTCGCCATGTGTGCCCAGCCGCTGGAGATGCTGGCCAAGCGCGATTTCAA
ACGGCTGGCGGTGGCCTGCGGCCTGAATGAGGCTGTGGTCAAAACCGCCATCGCGTTCATCGCCCGGCTCGAGCCCAAGC
CCGGCCGCCCCTTTGTGCAGGCCGAGCAAAACATCATCATCCCCGACGTGTTGGTGGTGAACACCAGCAAGACCGCCACG
CCCAAATTCCGCATCCAGCTCAACCCCGACGTGATGCCCAAGCTGCGTGTGCACGACATCTATGCCAACGCCCTGCGCGG
CGGCAAAGGCGACAACCACGCGGGCCTGCAGCAGCGCCTGCAAGAAGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCT
TTGACACCATCTTGCGCGTGTCCACCGCCATCGTGGAGCGGCAAAAAAACTTCCTCACGCACGGCGAGCTGGCCATGCGC
CCGCTGGTGCTGCGCGAGATCGCCGACGAGCTGGGCATGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAATACAT
GGCAACGCCGCAAGGCACGTTTGAGCTGAAGTACTTTTTTGGCTCGGGCCTAGGCACCGACAGCGGCAGCGCGGCGTCAA
GCACCGCGGTGCGGGCGCTCATCAAGCAGTTTGTCGCCGCCGAAAAACCCGCCAAGCCGCTGTCGGACAACCAGATTTCC
GAGATGCTGAAAGAGCAAGGCATCGACTGCGCCCGCCGCACGGTGGCCAAGTACCGCGAAGCCCTGAAGATCGCGCCCGC
CAACCTGCGCAAAACCCTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(356-511)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-341)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P0M6N7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.008

100

0.53

Multiple sequence alignment