Detailed information NTG0147465

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABH13_RS12315
• Genome accession: NZ_CP011686
• Coordinates: 2582147..2582761 (-)
• Length: 204 a.a.
• NCBI ID: WP_020956097.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain G341
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2577147..2587761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABH13_RS12290 (ABH13_2493)	rpsT	2577450..2577716 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ABH13_RS12295 (ABH13_2494)	holA	2577733..2578774 (-)	1042	Protein_2416	DNA polymerase III subunit delta	-
ABH13_RS20545	-	2578814..2578965 (-)	152	Protein_2417	hypothetical protein	-
ABH13_RS20080 (ABH13_2495)	-	2579006..2579140 (+)	135	WP_041481886.1	YqzM family protein	-
ABH13_RS12305 (ABH13_2496)	comEC	2579159..2581510 (-)	2352	WP_047935969.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABH13_RS12310 (ABH13_2497)	-	2581511..2582080 (-)	570	WP_007408264.1	ComE operon protein 2	-
ABH13_RS12315 (ABH13_2498)	comEA	2582147..2582761 (-)	615	WP_020956097.1	helix-hairpin-helix domain-containing protein	Machinery gene
ABH13_RS12320 (ABH13_2499)	comER	2582820..2583641 (+)	822	WP_020956098.1	late competence protein ComER	-
ABH13_RS12325 (ABH13_2500)	-	2583711..2584448 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
ABH13_RS12330 (ABH13_2501)	rsfS	2584445..2584801 (-)	357	WP_007408260.1	ribosome silencing factor	-
ABH13_RS12335 (ABH13_2502)	yqeK	2584819..2585379 (-)	561	WP_007408259.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ABH13_RS12340 (ABH13_2503)	-	2585369..2585938 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
ABH13_RS12345 (ABH13_2504)	yhbY	2585949..2586239 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ABH13_RS12350 (ABH13_2505)	aroE	2586233..2587075 (-)	843	WP_007408256.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22251.45 Da
• Isoelectric Point: 6.4940

>NTDB_id=147465 ABH13_RS12315 WP_020956097.1 2582147..2582761(-) (comEA) [Bacillus velezensis strain G341]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=147465 ABH13_RS12315 WP_020956097.1 2582147..2582761(-) (comEA) [Bacillus velezensis strain G341]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363