Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   AA971_RS00050 Genome accession   NZ_CP011482
Coordinates   9051..11414 (+) Length   787 a.a.
NCBI ID   WP_064431745.1    Uniprot ID   -
Organism   Helicobacter pylori strain L7     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4051..16414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AA971_RS00020 (AA971_00015) groES 4086..4442 (-) 357 WP_033767138.1 co-chaperone GroES -
  AA971_RS00025 (AA971_00020) dnaG 4716..6395 (+) 1680 WP_064431742.1 DNA primase -
  AA971_RS00030 (AA971_00025) - 6392..7444 (+) 1053 WP_064431743.1 MnmA/TRMU family protein -
  AA971_RS00035 (AA971_00030) - 7533..8360 (+) 828 WP_064431744.1 DUF5718 family protein -
  AA971_RS00040 (AA971_00035) comB2 8505..8786 (+) 282 WP_001272686.1 TrbC/VirB2 family protein Machinery gene
  AA971_RS00045 (AA971_00040) comB3 8786..9049 (+) 264 WP_000584973.1 hypothetical protein Machinery gene
  AA971_RS00050 (AA971_00045) comB4 9051..11414 (+) 2364 WP_064431745.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  AA971_RS00060 (AA971_00050) - 11735..13075 (+) 1341 WP_064431746.1 COG3014 family protein -
  AA971_RS00065 (AA971_00055) cheV1 13173..14138 (+) 966 WP_000785505.1 chemotaxis protein CheV1 -
  AA971_RS00070 (AA971_00060) nspC 14135..15352 (+) 1218 WP_064431747.1 carboxynorspermidine decarboxylase -
  AA971_RS00075 (AA971_00065) lpxE 15362..15895 (-) 534 WP_230381437.1 lipid A 1-phosphatase LpxE -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90297.89 Da        Isoelectric Point: 8.7203

>NTDB_id=146188 AA971_RS00050 WP_064431745.1 9051..11414(+) (comB4) [Helicobacter pylori strain L7]
MLEKLLSAIKQKVSNYFLGVLPKSYSMSEENNLLGLYDEHFLLTKNENLVGILRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVVRLVVKRRKIDHQQNIQSDSKYLQAILNQFENKEVYENQYFLVLESAHSLQGVLEHKKKSLMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLSSKEVLNFYAEYINGFDLPLKPLVGGYLSDSYIASSITFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAIGALLYQETPLDIIFSIEPMSVNKTLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNILIKAPSLEDLDTQTSLVLGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGQSFSINPFCLEPNLQNLEFLQSFFLSMFDLAPSRDKEALEDMNAIFSAIKSLYETLYPKAFSLLDF
KETLKRTSSNQLGLSLEPYLNNPLFNALNDALNSNAFLNVINLDTITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNAKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHHFNVPLSETEIS
FLENTPLYARQVLVKNLGNGNSNMIDVSLESLGRYLKIFNSDSSHVNKVKALQKDYPTEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=146188 AA971_RS00050 WP_064431745.1 9051..11414(+) (comB4) [Helicobacter pylori strain L7]
ATGTTAGAAAAGCTTTTGAGCGCTATCAAACAAAAAGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACCTTTTAGGCTTGTATGATGAGCATTTTTTACTTACTAAAAACGAAAATTTAGTGGGCATTCTCC
GTTTAGAGGGAGTGAGCTACACCCATTTAAGCACAGAGCAATTGCAAGATCTTTTCACCGAGCGCCAGATGGCGTTGGAT
TCTTTAGAAAAAGTCGTGGTGCGCCTTGTGGTTAAAAGGCGTAAAATTGATCACCAACAAAACATTCAATCTGACTCTAA
ATACTTGCAAGCGATTTTGAATCAATTTGAAAATAAAGAAGTGTATGAGAATCAGTATTTTTTAGTTTTAGAAAGCGCGC
ACTCTTTACAGGGCGTTTTAGAGCATAAGAAAAAATCTCTCATGCATGCCAATAGGGAAAATTTTAAGGACATCCTCTCT
TATAAAGCGCATTTTTTGCAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAACTATGCCCCCAAACTCTTAAGCTC
CAAAGAGGTTTTGAATTTTTATGCGGAATACATTAACGGGTTTGACCTCCCTTTAAAACCCTTAGTAGGGGGGTATTTGA
GCGATAGCTATATCGCTAGTTCTATCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAATCAAAAAACCTATAAC
CGCTTGATTGGCATTAAAGCTTATGAGAGCGAGCGCATCACTTCTATAGCGATCGGAGCGCTTTTATACCAAGAAACGCC
TTTAGACATTATCTTTTCTATAGAGCCTATGAGCGTCAATAAAACGCTGAGTTTTTTAAAAGAACGGGCCAAATTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTGCTAGAATACCAAGAACTAGTCAAAACCAAACGCCTATCTATGCAAAAATTCGCC
CTAAACATTCTTATCAAAGCCCCTAGTTTAGAGGATTTAGATACTCAAACAAGCTTGGTTTTAGGGCTTTTATTTAAAGA
AAATTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAAGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACCTCTAAAGCCTTAGCGTGTTTAATGGTGTTTGAAAGGCAAAATTTAGGCTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAACAACGCTAAAGAAATCGCCAGAGTGAATGGGCATACTCTAATCATAGGGGCTACCGGGAGCGGTAAAAGCA
CGCTGATTAGCTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCATTTGATAGGATGCAAGGGCTG
TATTCTTTCACAAAATTTTTTAAAGGGCATTACCATGACGGCCAATCTTTTAGCATCAACCCCTTTTGTTTAGAGCCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTCTTTTTGAGCATGTTTGATCTTGCCCCTTCAAGGGATAAAGAAGCCTTAG
AAGACATGAATGCGATTTTTAGCGCGATTAAGAGCCTTTATGAGACCTTATACCCTAAAGCTTTTAGTTTGCTAGACTTT
AAAGAGACGCTTAAAAGAACCTCATCTAACCAATTGGGCTTGAGTTTAGAGCCGTATTTGAATAACCCCCTTTTTAACGC
TTTGAATGACGCACTCAATTCCAACGCTTTTTTAAATGTGATAAACCTAGACACCATCACCCAAAACCCTAAAGACTTGG
GGCTTTTAGCCTATTATTTGTTTTATAAGATCTTAGAAGAATCCAGGAAAAACGACAGCGGTTTTTTGGTTTTTTTAGAC
GAGTTTAAATCCTATGTGGAAAACGATTTACTGAACGCTAAAATTAACGCTTTAATCACGCAAGCCAGAAAAGCTAATGG
CGTGGTGGTGTTGGCCTTGCAAGACATTTACCAATTAAGCGGGGTCAAAAACGCTCATAGTTTTTTAAGCAACATGGGGA
CTCTTATTTTGTACCCGCAAAAAAACGCTAGGGAGTTGAAACACCACTTCAATGTGCCTTTGAGCGAAACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAGGTTTTAGTCAAAAATCTGGGTAACGGGAATTCTAACATGATTGATGT
GAGTTTGGAAAGTTTAGGGCGTTATTTGAAAATCTTTAATTCGGATTCTAGCCATGTGAACAAAGTGAAAGCGTTACAAA
AAGACTACCCTACAGAGTGGCGTGAGAAACTTTTGAAGAGCTAG

Domains

Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.967

100

0.98

Multiple sequence alignment