Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   IJ00_RS13485 Genome accession   NZ_CP011382
Coordinates   3208685..3210703 (-) Length   672 a.a.
NCBI ID   WP_035153815.1    Uniprot ID   A0A0T7BSR9
Organism   Calothrix sp. 336/3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3203685..3215703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IJ00_RS13460 (IJ00_13450) - 3203888..3204892 (+) 1005 WP_035153807.1 saccharopine dehydrogenase-like oxidoreductase -
  IJ00_RS13465 (IJ00_13455) - 3204965..3205516 (-) 552 WP_035153809.1 hypothetical protein -
  IJ00_RS13475 (IJ00_13465) - 3205790..3207001 (-) 1212 WP_035153811.1 type II secretion system F family protein -
  IJ00_RS13480 (IJ00_13470) pilT 3207405..3208529 (-) 1125 WP_035153813.1 type IV pilus twitching motility protein PilT Machinery gene
  IJ00_RS13485 (IJ00_13475) pilB/pilB1 3208685..3210703 (-) 2019 WP_035153815.1 GspE/PulE family protein Machinery gene
  IJ00_RS13490 (IJ00_13480) grpE 3210935..3211669 (+) 735 WP_082127325.1 nucleotide exchange factor GrpE -
  IJ00_RS13495 (IJ00_13485) dnaK 3211945..3213903 (+) 1959 WP_035153819.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 672 a.a.        Molecular weight: 75083.64 Da        Isoelectric Point: 5.1786

>NTDB_id=145329 IJ00_RS13485 WP_035153815.1 3208685..3210703(-) (pilB/pilB1) [Calothrix sp. 336/3]
MTYSSPQRRSTALTTRTEFSPFGNKLVQSGFVNGDQMRQALIESRKSGRPLTEVLESISGRQLSPELLRQYKKQQLFELK
ILYGVESLDPEVRAIGDTVVGNLIDTLIPVDICRRHRLVPLAKHEDQNPPSVLVAMVDPDNLEASDDLNRILRPQGWSLQ
RMVITQEDYQQLINQYLDELAVKQKHIDQEKFTDINQDLENLDNLNLEDAPEESEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEESLRIRFRKDGVLREAFPSLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSATQLGLDKLITDPETLSIVQEMVSRPFGLILVTGPTGSGKTTSLYSALSERNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFANALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCPDCRIPYTPTPEELARYGLTTSTEDVGLTFYKANTLSTEQIHEAKVSGGHICPTC
NGVGYKGRSGVYEVMRVSENIQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVDRVTFTDTGLEAELKA
KRKSGLTCRTCHAELKPEWLDCPYCMTSRFQD

Nucleotide


Download         Length: 2019 bp        

>NTDB_id=145329 IJ00_RS13485 WP_035153815.1 3208685..3210703(-) (pilB/pilB1) [Calothrix sp. 336/3]
ATGACATACTCGTCACCACAACGGCGCAGCACAGCTCTCACCACAAGAACAGAGTTTTCGCCTTTCGGAAATAAACTTGT
GCAGTCAGGTTTTGTCAATGGCGACCAGATGCGGCAAGCTCTGATTGAAAGTCGCAAATCTGGCAGACCTTTAACGGAAG
TACTTGAATCGATATCGGGGCGGCAATTATCACCGGAATTACTGAGACAATATAAAAAACAACAATTATTTGAACTTAAA
ATACTATACGGCGTTGAGTCATTAGATCCGGAAGTGAGAGCCATCGGTGACACAGTAGTGGGTAATCTAATTGACACTTT
GATTCCCGTAGATATCTGTCGTCGTCATCGCTTGGTTCCCCTAGCAAAACACGAAGACCAAAACCCCCCTTCAGTGCTAG
TAGCGATGGTAGATCCAGATAACCTGGAGGCTTCTGATGATTTGAATCGCATACTACGTCCTCAAGGTTGGTCTTTACAA
CGAATGGTAATCACTCAGGAGGATTATCAACAGTTAATTAATCAGTATCTGGATGAATTAGCTGTCAAACAAAAGCACAT
CGACCAGGAAAAATTTACAGATATTAATCAAGATTTAGAAAATCTAGATAACCTAAATCTTGAGGATGCTCCGGAGGAAT
CAGAAGCAGATTTAGGTTCTGCGATGAAGGGTGCAGAGGATGCTCCTGTAATTAATCTGGTGAATCGCATCCTGGCAAAA
GCATTACACGAAAAAGTATCGGATATTCACATTGAACCACAGGAAGAAAGTCTCCGGATTCGTTTTCGCAAGGATGGGGT
ACTCAGGGAAGCATTCCCATCTCTACCGAAAAAGATTATCCCGGCGGTGACAGCGCGGTTTAAGATTATTTCTAACTTGG
ATATTGCCGAACGTCGCTTACCCCAGGATGGCAGAATCCGTCGGATGTTTGAAGGACGGAAGGTAGATTTTCGTGTTAAC
ACCTTACCTAGTCGTTATGGGGAAAAGGTTGTATTACGGATTCTGGATAACTCTGCAACTCAACTAGGACTAGATAAGTT
AATTACTGACCCAGAAACCCTGAGTATCGTCCAAGAAATGGTCAGTCGTCCCTTCGGCTTAATTCTGGTGACAGGTCCGA
CTGGTTCTGGTAAAACAACTTCACTTTACTCTGCCCTATCTGAACGCAACGACCCAGGAATTAATATTAGTACGGTAGAA
GACCCAATTGAGTATAGTTTGCCCGGTATTACCCAGGTACAAGTAATTCGCGAGAAGGGGTTAGATTTTGCCAATGCACT
ACGGGCATTTTTGCGGCAGGATCCTGACGTGCTACTGGTGGGTGAAACACGGGATAAGGAGACAGCCAAAACAGCTATTG
AAGCTGCATTGACAGGTCACTTAGTTCTCACAACTCTACATACCAATGATGCACCGGGGGCGATCGCTCGTCTCGGTGAA
ATGGGTATCGAACCTTTTATGGTGTCTAGTTCTCTGATTGGTGTACTTGCACAACGTCTAGTCCGTCGTGTTTGTCCTGA
CTGTCGTATTCCCTATACTCCCACGCCAGAAGAACTTGCCCGTTATGGTCTAACTACTTCCACAGAAGATGTTGGTCTGA
CTTTTTATAAAGCTAACACTTTGAGTACGGAGCAAATTCACGAGGCAAAAGTCAGTGGTGGTCACATTTGTCCTACCTGT
AATGGTGTTGGTTATAAGGGACGTAGTGGTGTCTATGAAGTGATGCGCGTCTCGGAAAATATCCAAACCCTGATCAATGA
AGAAGCACCTACTGAACGTATTAAGGAAGTAGCTGTGGAAGAAGGGATGAAAACCCTCTTAGCATACAGTTTGGATTTGG
TTAGACAAGGTGCAACCACTTTAGAAGAAGTTGATCGTGTCACCTTCACTGATACAGGTTTGGAAGCAGAACTTAAAGCT
AAACGCAAGAGCGGTTTGACTTGTCGGACTTGCCATGCAGAATTGAAACCCGAATGGTTAGATTGCCCTTACTGCATGAC
ATCAAGATTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T7BSR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

66.568

100

0.67
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.066

90.625

0.363

Multiple sequence alignment