Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   VL20_RS13215 Genome accession   NZ_CP011339
Coordinates   2755725..2758358 (+) Length   877 a.a.
NCBI ID   WP_052276771.1    Uniprot ID   A0A0K1S259
Organism   Microcystis panniformis FACHB-1757     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750725..2763358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VL20_RS13190 (VL20_3209) - 2750998..2752185 (+) 1188 WP_052276769.1 RNA-guided endonuclease TnpB family protein -
  VL20_RS13195 (VL20_3210) - 2752567..2752848 (+) 282 WP_052276770.1 hypothetical protein -
  VL20_RS28620 - 2753583..2754231 (+) 649 Protein_2767 RNA-guided endonuclease InsQ/TnpB family protein -
  VL20_RS13205 (VL20_3214) pilH 2754648..2755013 (+) 366 WP_002785387.1 PleD family two-component system response regulator Machinery gene
  VL20_RS13210 (VL20_3215) - 2755105..2755635 (+) 531 WP_002757615.1 chemotaxis protein CheW -
  VL20_RS13215 (VL20_3216) pilJ 2755725..2758358 (+) 2634 WP_052276771.1 methyl-accepting chemotaxis protein Machinery gene
  VL20_RS13220 (VL20_3217) - 2758432..2759643 (+) 1212 WP_052276772.1 glycosyltransferase -
  VL20_RS13225 (VL20_3218) - 2760093..2761430 (+) 1338 WP_052276773.1 AAA-like domain-containing protein -

Sequence

Protein


Download         Length: 877 a.a.        Molecular weight: 95401.09 Da        Isoelectric Point: 4.4003

>NTDB_id=144708 VL20_RS13215 WP_052276771.1 2755725..2758358(+) (pilJ) [Microcystis panniformis FACHB-1757]
MPSGTEYAKLYRKANTAYCQGNLEDAAVIVKEMISKYPEDANVMLLQGHIHLGLQQYRLAEERYEKVLQLAKNSPNSSDL
ADYAQRGLDQIRQLRFEAEEGDFTIAATESIAYSEAAFDLGQGFNHSRPRKSQGADPDGQNKGLDWNSSLFNEEDPSEPT
VSKVHSYDDDFEESEETAFSSLTLPGPGSHGSQWSSDSAEGEAPFPFLEAAEIDSLEEGWDGELGSTGEATFMVSSDLGG
NPLTGRDQELSYRDTLEESTFAIDLESTHPQGAKTGLNQGRVTQKRYEEDETGDFGQPELNDEDLDGFSPLALTDTVQGL
GDWELFTGSGGEALSGGFLPEPGARLNASSGVGEPSFNPSSLKIPNDKRDSHLTHASEHLLKPVVEVNPGKLGFFVNASL
GKKQLILAALAGITPVVLIFAVSTTSWLSAKVAPKPAPIPPAPASLSPWSQPKPAMMLLTGLGTFALTWLGLQLLISQIK
RSLNDLQSQFDHLSEGNFNAKATIYSEDEFGQLANRFNQMARVVVITTTEAQRRAAETEREREDLQRQVIRLLDDVEGAA
RGDLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQGSTNSESFARNQSSDALRMAEELAVTLNSVQMMTD
SIQRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRETVSETARKVKRLAEASQEISKIVAVVSQIASRTNLLAL
NASIQAARAGEAGRGFAVVADEVRQLADRAAKSLKEIEQIVLQIQSETGSVMMAMEEGIQQVIDVTERSEQAKKSLEDII
QVSNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQRVAGSLQKLVSISRELLSSVERFKVDSEDDR

Nucleotide


Download         Length: 2634 bp        

>NTDB_id=144708 VL20_RS13215 WP_052276771.1 2755725..2758358(+) (pilJ) [Microcystis panniformis FACHB-1757]
ATGCCGTCAGGGACAGAATACGCAAAACTCTATAGAAAGGCTAACACCGCCTATTGCCAGGGCAACCTTGAGGATGCCGC
CGTCATTGTCAAAGAGATGATCAGCAAGTATCCCGAGGATGCCAATGTCATGCTTTTACAGGGTCACATTCATCTAGGAC
TACAACAATACAGATTGGCGGAGGAGCGTTACGAAAAAGTGCTGCAATTGGCTAAAAATTCTCCCAATAGTTCCGATCTA
GCCGATTATGCCCAGCGTGGTCTCGATCAGATTCGGCAATTGCGTTTTGAAGCCGAGGAGGGAGATTTTACGATCGCTGC
TACGGAAAGCATTGCCTACAGCGAGGCAGCGTTCGATCTAGGTCAAGGTTTTAACCATTCTCGCCCCAGGAAGTCGCAAG
GGGCTGACCCCGATGGACAAAATAAAGGGCTGGATTGGAATAGTAGTCTTTTCAATGAGGAGGATCCCAGCGAGCCTACG
GTAAGTAAAGTCCATTCCTATGACGATGATTTTGAGGAATCGGAGGAAACCGCCTTTAGTTCCCTTACCCTGCCGGGCCC
TGGCTCTCACGGTTCCCAGTGGTCTAGCGATTCGGCGGAGGGGGAAGCCCCTTTCCCCTTTTTAGAAGCGGCGGAGATTG
ACTCGCTGGAGGAAGGGTGGGACGGTGAGTTAGGGTCAACGGGAGAAGCCACTTTTATGGTTTCCTCTGATCTGGGGGGA
AATCCCCTGACCGGTAGAGATCAGGAGTTATCCTATAGGGATACCCTAGAAGAAAGCACTTTTGCCATTGACCTGGAATC
GACCCACCCACAAGGGGCAAAAACTGGCCTTAATCAGGGCAGAGTCACTCAAAAAAGATACGAGGAAGACGAAACCGGAG
ATTTTGGGCAACCAGAACTCAATGATGAGGATTTAGACGGTTTTTCCCCACTGGCTCTGACGGATACGGTTCAAGGACTG
GGGGACTGGGAGCTATTTACCGGTTCGGGGGGGGAAGCTTTAAGTGGTGGTTTTCTCCCCGAACCCGGTGCGCGCCTTAA
TGCCAGTTCTGGTGTGGGAGAGCCTAGTTTTAATCCTTCTAGTCTAAAAATCCCCAATGACAAAAGAGATAGTCATCTTA
CCCATGCTTCCGAGCACTTGCTCAAGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGGCTTTTTTGTCAACGCTTCTTTA
GGCAAAAAGCAATTAATTTTAGCGGCTTTAGCGGGTATTACCCCGGTGGTGTTAATTTTTGCCGTTAGCACCACCTCTTG
GCTTTCTGCAAAAGTTGCCCCCAAACCAGCCCCAATACCACCAGCACCAGCCAGCCTTTCTCCTTGGAGTCAACCGAAAC
CAGCAATGATGTTATTAACTGGATTGGGAACCTTTGCCCTGACTTGGCTAGGTTTACAGTTGCTAATTAGTCAGATTAAG
CGCAGTCTCAACGATCTGCAAAGCCAATTCGATCATCTCTCGGAAGGTAATTTTAACGCTAAAGCGACGATCTACTCGGA
AGATGAATTCGGTCAACTGGCCAATCGTTTCAATCAGATGGCTCGGGTGGTGGTAATTACCACCACGGAAGCCCAACGCC
GCGCCGCCGAAACGGAACGGGAACGGGAAGACTTACAACGGCAAGTGATTCGACTTCTCGATGATGTGGAAGGAGCGGCT
CGGGGAGATCTGACCGTGGAAGCGGAGGTAAGCGCCGATGTTTTAGGGGCGGTGGCCGATGCTTTTAATTTGACGATCCA
AAACCTGCGCGAAATTGTCCGACAGGTGAAAAAAGCGGCAAAACAGGTGAATCAAGGCAGTACCAATAGTGAATCTTTCG
CCCGCAATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAATTGGCCGTCACCCTTAATTCGGTACAGATGATGACCGAC
TCAATTCAACGGGTGGCGGAAAACGCCAGAGAAGCCGAAGAAGTCGCTCGCACTTCCTCGATTACCGCCCTGAAGGGGGG
CGATTCCGTGGAAAGAACCGTGGCGGGAATTCTACAAATCCGCGAGACCGTATCAGAAACGGCGCGAAAAGTCAAGCGTT
TGGCGGAAGCTTCCCAGGAAATCTCGAAAATTGTCGCGGTAGTCTCTCAGATTGCCTCGCGGACCAATTTATTGGCTCTT
AATGCCTCGATTCAAGCGGCGCGAGCAGGAGAGGCGGGCCGGGGTTTTGCGGTTGTGGCCGATGAGGTGCGTCAGTTGGC
CGATAGAGCCGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGATTCAATCGGAAACGGGTTCGGTAATGATGG
CGATGGAGGAAGGGATTCAACAGGTTATCGATGTCACCGAGCGCTCGGAACAGGCGAAGAAGTCCCTAGAAGATATTATC
CAAGTTTCTAACCGCATCGATACGCTGGTGCGATCGATTACCGCCGATACGGTTAAACAACGGGAAAATTCCCTCAATGT
CGCCCAAGTTATGCAGTCGGTGGAGTTAACTGCTCAGGAAACTTCCCAAGAATCCCAACGGGTGGCGGGTTCTCTGCAAA
AATTGGTGAGTATCTCCCGGGAGCTGCTTTCTTCGGTAGAACGGTTTAAGGTAGACTCAGAGGATGATCGATAA

Domains

Predicted by InterproScan.

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(654-834)

HAMP domain (HAMP)

(474-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K1S259

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

49.246

100

0.521

Multiple sequence alignment