Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FORC13_RS05030 Genome accession   NZ_CP011145
Coordinates   918858..919901 (+) Length   347 a.a.
NCBI ID   WP_001013170.1    Uniprot ID   A0A9Q5QS48
Organism   Bacillus cereus strain FORC_013     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 913858..924901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC13_RS04995 (FORC13_0891) - 914226..914714 (+) 489 WP_000764267.1 hypothetical protein -
  FORC13_RS05000 (FORC13_0892) - 914749..915060 (-) 312 WP_001093239.1 hypothetical protein -
  FORC13_RS05005 (FORC13_0893) - 915313..915528 (+) 216 WP_001008327.1 DUF3912 family protein -
  FORC13_RS05010 (FORC13_0894) - 915616..916008 (+) 393 WP_059303448.1 hypothetical protein -
  FORC13_RS05015 (FORC13_0895) - 916087..917481 (-) 1395 WP_001094345.1 L-cystine transporter -
  FORC13_RS05020 (FORC13_0896) - 917589..917831 (-) 243 WP_000440716.1 DUF2626 domain-containing protein -
  FORC13_RS05025 (FORC13_0897) - 917957..918652 (-) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  FORC13_RS05030 (FORC13_0898) comGA 918858..919901 (+) 1044 WP_001013170.1 competence type IV pilus ATPase ComGA Machinery gene
  FORC13_RS05035 (FORC13_0899) comGB 919888..920925 (+) 1038 WP_059303449.1 competence type IV pilus assembly protein ComGB -
  FORC13_RS05040 (FORC13_0900) comGC 920937..921236 (+) 300 WP_059303450.1 competence type IV pilus major pilin ComGC -
  FORC13_RS05045 (FORC13_0901) comGD 921233..921688 (+) 456 WP_059303451.1 competence type IV pilus minor pilin ComGD -
  FORC13_RS05050 (FORC13_0902) - 921681..921983 (+) 303 WP_000829464.1 hypothetical protein -
  FORC13_RS05055 comGF 921953..922423 (+) 471 WP_033692316.1 competence type IV pilus minor pilin ComGF -
  FORC13_RS05060 (FORC13_0904) comGG 922420..922791 (+) 372 WP_001231318.1 competence type IV pilus minor pilin ComGG -
  FORC13_RS05065 (FORC13_0905) - 922966..923616 (+) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  FORC13_RS05070 (FORC13_0906) - 923732..924229 (+) 498 WP_059303452.1 shikimate kinase -
  FORC13_RS05075 (FORC13_0907) - 924268..924468 (+) 201 WP_000106083.1 YqzE family protein -
  FORC13_RS31775 - 924732..924836 (-) 105 Protein_909 IS3 family transposase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39095.50 Da        Isoelectric Point: 8.7212

>NTDB_id=142724 FORC13_RS05030 WP_001013170.1 918858..919901(+) (comGA) [Bacillus cereus strain FORC_013]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEAGGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=142724 FORC13_RS05030 WP_001013170.1 918858..919901(+) (comGA) [Bacillus cereus strain FORC_013]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTTCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCAGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAGGCTAGCATATATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCTGGTGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

40.062

92.795

0.372

Multiple sequence alignment