Detailed information NTG0142327

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PCL1606_RS04795
• Genome accession: NZ_CP011110
• Coordinates: 1011571..1012443 (-)
• Length: 290 a.a.
• NCBI ID: WP_044465111.1
• Uniprot ID: A0A0C5ERL7
• Organism: Pseudomonas chlororaphis strain PCL1606
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1006571..1017443

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCL1606_RS04765 (PCL1606_09740)	-	1007476..1008912 (-)	1437	WP_045881212.1	undecaprenyl-phosphate glucose phosphotransferase	-
PCL1606_RS04770 (PCL1606_09750)	-	1009313..1009780 (+)	468	WP_044465116.1	hypothetical protein	-
PCL1606_RS04775 (PCL1606_09760)	-	1009777..1010466 (+)	690	WP_045881213.1	energy-coupling factor ABC transporter permease	-
PCL1606_RS04780 (PCL1606_09770)	-	1010531..1010746 (+)	216	WP_045881214.1	hypothetical protein	-
PCL1606_RS04785 (PCL1606_09780)	yacG	1010751..1010954 (-)	204	WP_044465113.1	DNA gyrase inhibitor YacG	-
PCL1606_RS04790 (PCL1606_09790)	coaE	1010951..1011574 (-)	624	WP_044465112.1	dephospho-CoA kinase	-
PCL1606_RS04795 (PCL1606_09800)	pilD	1011571..1012443 (-)	873	WP_044465111.1	prepilin peptidase	Machinery gene
PCL1606_RS04800 (PCL1606_09810)	pilC	1012446..1013663 (-)	1218	WP_044465110.1	type II secretion system F family protein	Machinery gene
PCL1606_RS04805 (PCL1606_09820)	-	1014023..1014439 (+)	417	WP_044465109.1	pilin	-
PCL1606_RS04810 (PCL1606_09830)	-	1014696..1015052 (+)	357	WP_044465108.1	BON domain-containing protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31839.09 Da
• Isoelectric Point: 8.2540

>NTDB_id=142327 PCL1606_RS04795 WP_044465111.1 1011571..1012443(-) (pilD) [Pseudomonas chlororaphis strain PCL1606]
MSLSEVLASAPLVFVLSTLVLGLIVGSFLNVLVWRLPKMLDQDWRAQAREILGLPAEPAGPTYNLLLPHSQCPHCAHPIR
PWENIPLLSYLLLRGRCSACKGPIGRRYPLVELACGLGSALVAWHFGFGWQAGLMLFLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFALFVSLHDALWGAVAGYLCLWSVYWVFKLLTGKEGIGHGDFKLLALLGAWGGWQILPLTLLLASLTG
ALMGVIMLRRQRAQMSTPIPFGPFLAIAGWIALLWGGQITDLYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=142327 PCL1606_RS04795 WP_044465111.1 1011571..1012443(-) (pilD) [Pseudomonas chlororaphis strain PCL1606]
ATGTCATTGAGTGAAGTCCTGGCCAGCGCCCCGCTGGTCTTCGTGTTATCGACGCTGGTCCTGGGCCTGATCGTCGGCAG
CTTCCTCAATGTGCTGGTCTGGCGCCTGCCGAAGATGCTCGACCAGGACTGGCGCGCCCAGGCCCGCGAGATCCTCGGGC
TGCCCGCCGAACCGGCCGGGCCGACCTACAACCTGCTGCTCCCCCACTCCCAATGCCCGCATTGCGCGCACCCGATCCGC
CCCTGGGAAAACATCCCGCTGCTCAGCTACCTGCTGCTGCGCGGCCGCTGCTCCGCCTGCAAGGGCCCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGGCCTGCGGCCTGGGCTCGGCGTTGGTGGCCTGGCATTTCGGTTTCGGCTGGCAGGCCGGGC
TGATGCTGTTCCTGAGCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCATCAACTGTTGCCGGACGTGCTGGTG
CTGCCCCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGCGCTGTTCGTCAGCCTGCACGACGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTGTACTGGGTGTTCAAGCTGCTGACCGGCAAGGAAGGGATCGGCCACGGCGATT
TCAAGCTGCTGGCGCTGCTTGGCGCCTGGGGCGGCTGGCAGATTCTGCCGCTGACACTGCTGCTGGCCTCGCTGACAGGC
GCGCTGATGGGGGTGATCATGCTGCGGCGGCAGCGGGCGCAGATGTCCACGCCCATTCCTTTCGGTCCCTTTCTGGCCAT
CGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACCTCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: A0A0C5ERL7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.521	97.931	0.524
pilD	Vibrio campbellii strain DS40M4	50	97.241	0.486
pilD	Acinetobacter baumannii D1279779	48.339	93.448	0.452
pilD	Acinetobacter nosocomialis M2	48.339	93.448	0.452
pilD	Neisseria gonorrhoeae MS11	49.012	87.241	0.428