Detailed information NTG0142226

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: bslm_RS18420
• Genome accession: NZ_CP011101
• Coordinates: 3699462..3700607 (+)
• Length: 381 a.a.
• NCBI ID: WP_046381403.1
• Uniprot ID: A0AA96ZUF3
• Organism: Bacillus sp. LM 4-2
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3694462..3705607

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
bslm_RS18395 (BsLM_3769)	-	3694477..3695757 (+)	1281	WP_046381400.1	pitrilysin family protein	-
bslm_RS18400 (BsLM_3770)	albG	3695754..3696455 (+)	702	WP_029726080.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
bslm_RS18405 (BsLM_3771)	-	3696461..3697837 (-)	1377	WP_046381401.1	YncE family protein	-
bslm_RS18410 (BsLM_3772)	-	3697877..3699232 (-)	1356	WP_046381402.1	YncE family protein	-
bslm_RS21820 (BsLM_3773)	-	3699229..3699459 (-)	231	WP_024572000.1	hypothetical protein	-
bslm_RS18420 (BsLM_3774)	rapF	3699462..3700607 (+)	1146	WP_046381403.1	response regulator aspartate phosphatase RapF	Regulator
bslm_RS18425	phrF	3700591..3700710 (+)	120	WP_041334633.1	phosphatase RapF inhibitor PhrF	Regulator
bslm_RS18430	-	3700808..3701270 (+)	463	Protein_3716	YbaK/EbsC family protein	-
bslm_RS18435 (BsLM_3776)	speB	3701302..3702174 (-)	873	WP_003227545.1	agmatinase	-
bslm_RS18440 (BsLM_3777)	speE	3702235..3703065 (-)	831	WP_003227543.1	spermidine synthase	-
bslm_RS18445 (BsLM_3778)	-	3703268..3705343 (+)	2076	WP_029726084.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45622.10 Da
• Isoelectric Point: 5.0624

>NTDB_id=142226 bslm_RS18420 WP_046381403.1 3699462..3700607(+) (rapF) [Bacillus sp. LM 4-2]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEQEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=142226 bslm_RS18420 WP_046381403.1 3699462..3700607(+) (rapF) [Bacillus sp. LM 4-2]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAAGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACAGGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	99.213	100	0.992
rapC	Bacillus subtilis subsp. subtilis str. 168	57.632	99.738	0.575