Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AzCIB_RS21300 Genome accession   NZ_CP011072
Coordinates   4748127..4749572 (+) Length   481 a.a.
NCBI ID   WP_050417736.1    Uniprot ID   -
Organism   Azoarcus sp. CIB     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4743127..4754572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AzCIB_RS21270 (AzCIB_4255) thiL 4743868..4744827 (+) 960 WP_050418496.1 thiamine-phosphate kinase -
  AzCIB_RS21275 (AzCIB_4256) - 4744849..4745328 (+) 480 WP_050417731.1 phosphatidylglycerophosphatase A -
  AzCIB_RS21280 (AzCIB_4257) - 4745333..4745812 (+) 480 WP_050417732.1 nicotinamide-nucleotide amidohydrolase family protein -
  AzCIB_RS21285 (AzCIB_4258) recA 4745898..4746932 (+) 1035 WP_050417733.1 recombinase RecA Machinery gene
  AzCIB_RS21290 (AzCIB_4259) recX 4746950..4747399 (+) 450 WP_050417734.1 recombination regulator RecX -
  AzCIB_RS21295 (AzCIB_4260) lptB 4747386..4748108 (+) 723 WP_050417735.1 LPS export ABC transporter ATP-binding protein -
  AzCIB_RS21300 (AzCIB_4261) rpoN/rpoN1 4748127..4749572 (+) 1446 WP_050417736.1 RNA polymerase factor sigma-54 Machinery gene
  AzCIB_RS21305 (AzCIB_4262) raiA 4749602..4749928 (+) 327 WP_050417737.1 ribosome-associated translation inhibitor RaiA -
  AzCIB_RS21310 (AzCIB_4263) ptsN 4750065..4750550 (+) 486 WP_050418497.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AzCIB_RS21315 (AzCIB_4264) hprK 4750519..4751460 (+) 942 WP_050417738.1 HPr(Ser) kinase/phosphatase -
  AzCIB_RS21320 (AzCIB_4265) ilvA 4751547..4753067 (+) 1521 WP_050417739.1 threonine ammonia-lyase, biosynthetic -
  AzCIB_RS21325 (AzCIB_4266) - 4753046..4753663 (-) 618 WP_083447086.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 53680.48 Da        Isoelectric Point: 4.8809

>NTDB_id=142040 AzCIB_RS21300 WP_050417736.1 4748127..4749572(+) (rpoN/rpoN1) [Azoarcus sp. CIB]
MKPSLQLKISQHLTLTPQLQQSIKLLQLSTLELNQELERFLLENPMLEREDGDGSDFSPSVAQVSPGTTESGSQSDTPAP
SEETRNEAEPANFDEAGEWSNAGSGSSNRNDDDDTDFQELQAAGTSLRDHLDQQVSLSPLSDRDRALVRFIMEALDEDGY
LNQSLDELLPLLPPELEYDLDDLTIALHHVQNLDPAGIGARTPQECLSLQLRAMPSCPTRDLALKIVDEHLDLLAERNFL
RIKRATGCDDDALRAAHALICRLDPHPGSQHSVQETRYILPDVVVRKVRNRWTVTLNPEAMPKLRINSLYASILQQNRGS
GGALTSQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDYGDVAMRPLTLREIADQLELHESTVSRVTTQKFMATPRG
VFELKYFFGSHVATDTGGAASSTAIRALIRQLVEAEDRKKPLSDAKIAELLGQQGIVVARRTIAKYRESLNIPPVSLRKA
I

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=142040 AzCIB_RS21300 WP_050417736.1 4748127..4749572(+) (rpoN/rpoN1) [Azoarcus sp. CIB]
ATGAAACCGTCACTCCAGCTCAAGATCTCGCAGCACCTCACGCTGACGCCGCAGCTGCAGCAATCGATCAAACTGCTGCA
GCTGTCGACGCTGGAGCTGAACCAGGAGCTGGAACGCTTCCTCCTCGAGAACCCCATGCTCGAGCGCGAGGACGGCGACG
GCAGCGACTTTTCTCCGAGCGTCGCACAGGTTTCGCCGGGCACGACCGAGTCCGGCAGCCAGTCCGACACCCCCGCACCG
AGCGAGGAGACGCGTAACGAGGCCGAGCCGGCAAACTTCGACGAGGCCGGCGAGTGGTCGAACGCGGGCAGCGGCTCGTC
GAACCGCAACGATGACGACGACACCGACTTCCAGGAACTGCAGGCCGCCGGCACCAGCCTGCGCGACCACCTCGACCAGC
AGGTGAGCCTGTCGCCGCTCTCCGATCGCGACCGCGCGCTGGTGCGCTTCATCATGGAGGCGCTCGACGAGGACGGCTAC
CTCAACCAGTCGCTCGATGAACTGCTGCCGCTGCTGCCGCCGGAACTCGAATACGACCTCGACGACCTCACGATCGCGCT
GCACCACGTGCAGAACCTCGACCCGGCCGGCATCGGCGCGCGCACGCCGCAGGAATGCCTGAGCCTGCAGCTCAGGGCGA
TGCCGTCCTGCCCGACGCGCGACCTCGCACTGAAGATCGTCGACGAACACCTCGACCTGCTCGCCGAGCGCAACTTCCTG
CGCATCAAGCGCGCCACCGGCTGCGACGACGACGCGCTGCGCGCCGCGCACGCGCTGATCTGCCGCCTCGACCCGCATCC
GGGCTCACAGCACTCGGTGCAGGAGACGCGCTACATCCTGCCCGACGTCGTCGTGCGCAAAGTGCGCAACCGCTGGACGG
TCACGCTCAACCCCGAGGCGATGCCCAAGCTGCGCATCAATTCCCTGTACGCCAGCATCCTGCAGCAGAACCGGGGATCG
GGCGGCGCGCTCACCAGCCAGCTACAGGAAGCGCGCTGGCTGATCAAGAATGTGCAACAACGTTTCGACACTATCCTGCG
GGTTTCCCAGGCGATCGTTGACCAGCAGCGGCAGTTCTTCGATTATGGCGATGTGGCGATGCGACCTCTGACGTTGAGGG
AAATCGCGGACCAGCTTGAGCTGCACGAGTCGACGGTCTCGCGAGTCACGACGCAGAAATTCATGGCCACACCGCGCGGC
GTGTTTGAGCTGAAGTACTTTTTCGGCAGCCACGTTGCCACCGACACCGGTGGGGCGGCATCTTCCACGGCGATTCGAGC
GCTGATTCGCCAGCTGGTGGAGGCCGAAGACAGGAAGAAGCCCCTGTCCGACGCGAAAATTGCCGAATTGTTGGGCCAGC
AGGGTATCGTGGTGGCACGCAGGACGATCGCGAAGTACCGCGAGTCACTCAATATCCCGCCGGTCAGCCTGCGCAAAGCG
ATCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-310)

Sigma-54, DNA binding domain (Sigma54_DBD)

(324-479)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.6

100

0.578

Multiple sequence alignment