Detailed information NTG0141870

Bioinformatically predicted

Overview

• Name: pepF
• Type: Regulator
• Locus tag: VN24_RS22390
• Genome accession: NZ_CP011058
• Coordinates: 4953597..4955396 (+)
• Length: 599 a.a.
• NCBI ID: WP_045673589.1
• Uniprot ID: A0A0D5NRM4
• Organism: Paenibacillus beijingensis strain DSM 24997
• Function: degradation of XIP; competence shut-off (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	4942066..4960260	4953597..4955396	within	0

Gene organization within MGE regions

Location: 4942066..4960260

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VN24_RS22330 (VN24_22330)	-	4942066..4942998 (+)	933	WP_045672227.1	3-hydroxyacyl-CoA dehydrogenase family protein	-
VN24_RS22335 (VN24_22335)	-	4943068..4944018 (+)	951	WP_052703088.1	3-keto-5-aminohexanoate cleavage protein	-
VN24_RS22340 (VN24_22340)	-	4944061..4944609 (+)	549	WP_045672228.1	gamma carbonic anhydrase family protein	-
VN24_RS22345 (VN24_22345)	-	4944617..4945657 (+)	1041	WP_420798636.1	FecCD family ABC transporter permease	-
VN24_RS22350 (VN24_22350)	-	4945657..4946676 (+)	1020	WP_045672230.1	FecCD family ABC transporter permease	-
VN24_RS22355 (VN24_22355)	-	4946704..4947276 (+)	573	WP_045673588.1	nitroreductase family protein	-
VN24_RS22360 (VN24_22360)	-	4947323..4948348 (+)	1026	WP_045672231.1	ABC transporter substrate-binding protein	-
VN24_RS22365 (VN24_22365)	-	4948519..4948899 (+)	381	WP_045672232.1	RidA family protein	-
VN24_RS22370 (VN24_22370)	-	4948940..4950043 (+)	1104	WP_045672233.1	threonine synthase	-
VN24_RS22375 (VN24_22375)	-	4950040..4951212 (+)	1173	WP_045672234.1	alpha/beta hydrolase family protein	-
VN24_RS22380 (VN24_22380)	-	4951199..4952311 (+)	1113	WP_045672235.1	alanine racemase	-
VN24_RS22385 (VN24_22385)	-	4952348..4953565 (+)	1218	WP_045672236.1	amidohydrolase family protein	-
VN24_RS22390 (VN24_22390)	pepF	4953597..4955396 (+)	1800	WP_045673589.1	oligoendopeptidase F	Regulator
VN24_RS22395 (VN24_22395)	-	4955439..4956512 (+)	1074	WP_045672237.1	M42 family metallopeptidase	-
VN24_RS22400 (VN24_22400)	-	4956553..4958021 (+)	1469	Protein_4408	M81 family metallopeptidase	-
VN24_RS22405 (VN24_22405)	-	4958122..4958667 (+)	546	Protein_4409	D-serine ammonia-lyase	-
VN24_RS22410 (VN24_22410)	-	4958846..4959700 (+)	855	WP_045672238.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 599 a.a.
• Molecular weight: 69284.35 Da
• Isoelectric Point: 5.6174

>NTDB_id=141870 VN24_RS22390 WP_045673589.1 4953597..4955396(+) (pepF) [Paenibacillus beijingensis strain DSM 24997]
MKKLPTRTEIQLEDTWRLEDIFSTDEEWENEYEAVKEFIPEVTEFKGKLADGAELLARALQRQDQLLVRLGKLYTYAQMR
NFQDTANAFYQGLYDRIKSLYSQAESTLSYIVPEILSIDEERIKGYLKESKELSLYKHALEEMNLQRPHVLSAEQEALLA
QASEVFDASSHTFGMINNADLEFPSIKDEDGKEVEVTHGRYKSFLESEDRRVRHEVFKAVYDTYGKFRNTFASTLAGNVK
KNNFHAQVRNYQSARHAALAANHIPESVYVNLVETIQKYLPLLHRYVKLRKKVLGVNELHVYDLYAPLMKNIKMKIGYHE
AKDFIIKGLAPLGEEYNQVLNEGFANRWVDVHENKGKRSGAFSLGAYGTNPYVLMNWQDNLKSLFTLAHEFGHSVHSYYT
SKTQPFRYGDYSTFVAEVASTCNESLLNDYLLKTIQDEQIRLYLLNHFLDAFQKTVFRQTMFAEFEHFIHIKSQNHEALT
ADLFTKEYDALNKKYFGAEDIVVDEEIGLEWARIPHFYMNYYVYQYATGFSAATALSKQILEEGAPAVERYIEFLKSGNS
DYPIEVLKKAGVDMTTAKPIEEACKVFEEKLAEMESLLS

Nucleotide

• Length: 1800 bp

>NTDB_id=141870 VN24_RS22390 WP_045673589.1 4953597..4955396(+) (pepF) [Paenibacillus beijingensis strain DSM 24997]
ATGAAAAAGCTTCCGACGAGAACGGAGATTCAATTAGAGGATACATGGAGATTAGAGGATATTTTTTCTACTGACGAAGA
GTGGGAAAATGAATACGAAGCGGTTAAAGAATTCATACCGGAAGTAACGGAGTTTAAAGGAAAACTTGCTGATGGGGCCG
AACTCCTTGCTCGTGCTTTACAAAGACAGGATCAATTATTAGTGCGTCTTGGAAAGCTCTATACCTATGCGCAAATGCGC
AATTTTCAAGATACGGCAAATGCATTTTATCAAGGGTTATATGATCGTATCAAAAGTCTCTATTCTCAAGCAGAAAGCAC
CCTTTCCTATATTGTACCGGAAATCCTTTCTATTGATGAAGAACGCATTAAAGGGTATTTAAAAGAAAGTAAAGAGCTTT
CTTTATATAAGCATGCGCTTGAAGAAATGAATTTGCAAAGACCCCATGTACTTTCGGCAGAGCAGGAAGCCTTATTGGCT
CAAGCTTCCGAAGTATTTGACGCTTCAAGTCATACATTTGGAATGATCAACAATGCCGACCTGGAATTTCCATCGATTAA
AGATGAAGATGGGAAGGAGGTAGAAGTTACGCATGGCCGCTATAAAAGCTTTCTTGAAAGCGAAGATCGAAGAGTTCGTC
ATGAGGTCTTTAAAGCTGTTTATGATACATACGGCAAGTTTCGCAATACCTTTGCGAGTACATTGGCTGGAAACGTCAAA
AAAAATAATTTTCATGCACAGGTTCGAAACTATCAATCAGCTCGCCATGCAGCGTTAGCCGCAAATCATATCCCGGAAAG
TGTCTATGTCAACCTTGTCGAAACGATTCAGAAATACTTGCCATTATTACACAGGTATGTAAAGCTGCGCAAAAAGGTGC
TGGGTGTAAACGAACTCCATGTCTATGATTTATATGCGCCGTTAATGAAAAATATAAAAATGAAGATTGGCTATCATGAA
GCAAAGGATTTCATTATAAAGGGGCTCGCTCCTCTTGGTGAGGAATATAATCAAGTATTAAATGAAGGGTTTGCCAACCG
TTGGGTAGATGTACATGAAAATAAAGGGAAAAGAAGCGGCGCTTTCTCATTGGGAGCGTATGGAACAAATCCATACGTAT
TAATGAATTGGCAGGATAATTTGAAAAGCCTTTTTACTTTAGCACATGAATTCGGACATTCTGTGCATAGCTACTACACT
AGTAAAACACAACCATTCCGTTATGGAGATTACTCGACCTTTGTTGCGGAGGTTGCCTCTACATGTAATGAATCGCTTTT
AAATGATTATTTATTAAAAACGATTCAGGATGAGCAGATCAGATTGTATTTATTAAATCATTTCTTAGACGCATTTCAAA
AAACAGTATTCCGTCAAACGATGTTTGCTGAATTCGAGCATTTCATCCATATAAAGTCTCAAAATCATGAAGCGTTAACA
GCAGATTTATTTACGAAAGAATACGATGCCTTAAATAAAAAGTATTTCGGTGCAGAAGACATTGTTGTTGATGAAGAAAT
CGGATTAGAATGGGCGCGTATCCCACACTTTTACATGAACTATTATGTGTACCAATATGCAACAGGCTTTAGTGCGGCAA
CGGCCTTAAGCAAACAAATTTTAGAAGAGGGAGCGCCGGCTGTAGAAAGATATATCGAATTTTTGAAATCAGGAAACTCC
GATTATCCGATTGAAGTGTTGAAGAAAGCTGGGGTAGATATGACAACTGCAAAACCTATCGAAGAAGCATGTAAAGTTTT
TGAGGAAAAGTTGGCTGAAATGGAAAGCTTACTATCGTAA

Domains

Predicted by InterproScan.

• Peptidase family M3 (Peptidase_M3) (204-585)

• Oligopeptidase F (Peptidase_M3_N) (114-183)

3D structure

• AlphaFold DB: A0A0D5NRM4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pepF	Streptococcus salivarius strain HSISS4	48.485	99.165	0.481