Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BIS30_RS02540 Genome accession   NZ_CP011051
Coordinates   441903..442520 (-) Length   205 a.a.
NCBI ID   WP_003226145.1    Uniprot ID   -
Organism   Bacillus spizizenii strain T30     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436903..447520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIS30_RS02515 (BIS30_02515) rpsT 437204..437470 (+) 267 WP_003226155.1 30S ribosomal protein S20 -
  BIS30_RS02520 (BIS30_02520) holA 437485..438528 (-) 1044 WP_003226154.1 DNA polymerase III subunit delta -
  BIS30_RS20765 - 438568..438717 (-) 150 WP_136975859.1 hypothetical protein -
  BIS30_RS02525 (BIS30_02525) - 438758..438892 (+) 135 WP_003226152.1 YqzM family protein -
  BIS30_RS02530 (BIS30_02530) comEC 438933..441263 (-) 2331 WP_003226149.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BIS30_RS02535 (BIS30_02535) - 441267..441836 (-) 570 WP_003226147.1 ComE operon protein 2 -
  BIS30_RS02540 (BIS30_02540) comEA 441903..442520 (-) 618 WP_003226145.1 helix-hairpin-helix domain-containing protein Machinery gene
  BIS30_RS02545 (BIS30_02545) comER 442604..443425 (+) 822 WP_003226143.1 late competence protein ComER -
  BIS30_RS02550 (BIS30_02550) - 443491..444234 (-) 744 WP_003226141.1 class I SAM-dependent methyltransferase -
  BIS30_RS02555 (BIS30_02555) rsfS 444231..444587 (-) 357 WP_003226139.1 ribosome silencing factor -
  BIS30_RS02560 (BIS30_02560) yqeK 444605..445165 (-) 561 WP_003226137.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BIS30_RS02565 (BIS30_02565) - 445155..445724 (-) 570 WP_003226135.1 nicotinate-nucleotide adenylyltransferase -
  BIS30_RS02570 (BIS30_02570) yhbY 445736..446026 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  BIS30_RS02575 (BIS30_02575) aroE 446020..446862 (-) 843 WP_003226130.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21919.78 Da        Isoelectric Point: 5.1470

>NTDB_id=141761 BIS30_RS02540 WP_003226145.1 441903..442520(-) (comEA) [Bacillus spizizenii strain T30]
MNWLNHHKKAIILGASAAVFIAIIFFLASGKNKESMKQTVSTETENTEVKQEAVKDESNETIVIDIKGAVKHPGVYEMRT
GDRLSQAIEKAGGTSEQADEMQVNLAGLLQDGTVVYIPKRGEETAVQQGVGGAIQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=141761 BIS30_RS02540 WP_003226145.1 441903..442520(-) (comEA) [Bacillus spizizenii strain T30]
ATGAATTGGTTGAATCATCATAAGAAAGCAATTATTTTAGGGGCCTCTGCGGCTGTTTTCATAGCGATCATTTTCTTCCT
GGCCAGCGGGAAAAACAAAGAGTCGATGAAGCAGACTGTATCAACTGAGACAGAAAATACGGAGGTAAAGCAGGAAGCAG
TCAAAGATGAGTCAAACGAAACGATTGTGATTGACATCAAAGGTGCTGTTAAGCACCCTGGTGTTTATGAAATGCGAACA
GGGGACAGATTATCCCAGGCAATTGAGAAAGCCGGCGGGACCAGTGAACAAGCAGACGAAATGCAAGTGAATTTGGCGGG
GCTGCTGCAGGACGGGACAGTGGTGTACATCCCGAAGAGGGGTGAGGAGACAGCGGTGCAGCAAGGAGTCGGAGGCGCCA
TCCAAAGCGATGGAGGAAAGGGGGCGCTGGTAAATATCAATACAGCAACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAATGGGCGTTTCCAGACAATTGAAGATATTACGAAGGT
TTCAGGGATCGGTGAAAAGTCGTTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

89.268

100

0.893
  comEA Latilactobacillus sakei subsp. sakei 23K

31.646

100

0.366
  comEA Staphylococcus aureus MW2

37.688

97.073

0.366

Multiple sequence alignment