Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   FORC12_RS04150 Genome accession   NZ_CP010998
Coordinates   830047..830733 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain FORC_012 isolate human sputum     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825047..835733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC12_RS04130 (FORC12_0771) rpsT 825961..826212 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  FORC12_RS04135 (FORC12_0772) holA 826257..827231 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  FORC12_RS04140 (FORC12_0773) comEC 827288..829489 (-) 2202 WP_020978202.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  FORC12_RS04145 (FORC12_0774) - 829494..829955 (-) 462 WP_000439693.1 ComE operon protein 2 -
  FORC12_RS04150 (FORC12_0775) comEA 830047..830733 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  FORC12_RS04155 (FORC12_0776) - 830773..831489 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  FORC12_RS04160 (FORC12_0777) rsfS 831492..831845 (-) 354 WP_001088022.1 ribosome silencing factor -
  FORC12_RS04165 (FORC12_0778) yqeK 831846..832430 (-) 585 WP_001019323.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  FORC12_RS04170 (FORC12_0779) nadD 832420..832989 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  FORC12_RS04175 (FORC12_0780) yhbY 832992..833282 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  FORC12_RS04180 (FORC12_0781) aroE 833286..834092 (-) 807 WP_000666757.1 shikimate dehydrogenase -
  FORC12_RS04185 (FORC12_0782) yqeH 834106..835206 (-) 1101 WP_001280138.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=141170 FORC12_RS04150 WP_001792568.1 830047..830733(-) (comEA) [Staphylococcus aureus strain FORC_012 isolate human sputum]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=141170 FORC12_RS04150 WP_001792568.1 830047..830733(-) (comEA) [Staphylococcus aureus strain FORC_012 isolate human sputum]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment