Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   N805_RS19010 Genome accession   NZ_CP010979
Coordinates   4305848..4306714 (+) Length   288 a.a.
NCBI ID   WP_019473262.1    Uniprot ID   A0AAU8S7L8
Organism   Pseudomonas putida S13.1.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4300848..4311714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N805_RS18990 (N805_18995) - 4302209..4303699 (-) 1491 WP_019473264.1 tyrosine-type recombinase/integrase -
  N805_RS19000 (N805_19005) - 4304010..4304420 (-) 411 WP_016484801.1 pilin -
  N805_RS19005 (N805_19010) - 4304637..4305845 (+) 1209 WP_019473263.1 type II secretion system F family protein -
  N805_RS19010 (N805_19015) pilD 4305848..4306714 (+) 867 WP_019473262.1 prepilin peptidase Machinery gene
  N805_RS19015 (N805_19020) coaE 4306714..4307337 (+) 624 WP_019473261.1 dephospho-CoA kinase -
  N805_RS19020 (N805_19025) yacG 4307334..4307534 (+) 201 WP_016501579.1 DNA gyrase inhibitor YacG -
  N805_RS19025 (N805_19030) - 4307771..4308454 (-) 684 WP_019473260.1 energy-coupling factor ABC transporter permease -
  N805_RS19030 (N805_19035) - 4308671..4309300 (+) 630 WP_019473259.1 DUF1780 domain-containing protein -
  N805_RS30095 - 4309433..4309605 (+) 173 Protein_3823 DUF3094 family protein -
  N805_RS19035 (N805_19040) - 4309690..4310985 (+) 1296 WP_019473258.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31865.07 Da        Isoelectric Point: 7.5974

>NTDB_id=141075 N805_RS19010 WP_019473262.1 4305848..4306714(+) (pilD) [Pseudomonas putida S13.1.2]
MTLWTLLAEQPAYFLTLATVLGLLVGSFLNVLVYRLPIMLERQWQREAQEVLGLPLAQHERFDLCLPASRCPHCAHQIRA
WENIPVLSYLALRGRCSACKARISPRYPLVEVASALLSLVVAWRFGASAEALLALPLTWCLLALSLIDADHQLLPDVLVL
PMLWLGLIVNAFGLYVPLADALWGAVAGYLSLWTVYWVFKLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LVGLCLLRLRRHAMGTAIPFGPYLAIAGWIAVLWGDEMYASYMQLLGY

Nucleotide


Download         Length: 867 bp        

>NTDB_id=141075 N805_RS19010 WP_019473262.1 4305848..4306714(+) (pilD) [Pseudomonas putida S13.1.2]
ATGACTTTATGGACTTTGCTGGCTGAGCAGCCGGCGTACTTCCTCACCCTGGCCACGGTGCTCGGCTTGCTGGTGGGCAG
CTTTCTCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGCCAGTGGCAGCGTGAGGCGCAAGAAGTATTAGGGC
TGCCGCTTGCGCAACACGAACGGTTCGACCTGTGCCTGCCGGCATCCCGCTGCCCGCACTGCGCGCATCAGATTCGCGCC
TGGGAGAACATTCCGGTGCTCAGCTACCTGGCGTTGCGCGGGCGGTGCTCAGCCTGCAAAGCCCGTATCAGCCCGCGCTA
CCCACTGGTGGAGGTGGCCAGCGCGCTGCTGTCGCTGGTGGTGGCCTGGCGTTTTGGCGCTTCGGCCGAAGCCTTGCTGG
CATTGCCGCTCACCTGGTGCCTGCTGGCCCTGAGCCTGATCGATGCGGACCACCAGCTGTTGCCGGATGTGCTGGTGCTG
CCGATGCTGTGGCTTGGGCTGATCGTGAACGCCTTTGGCCTCTATGTGCCACTGGCCGACGCGTTGTGGGGCGCGGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAAGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCA
AGCTGATGGCGCTGATCGGGGCCTGGGGCGGTTGGCAGGTGTTGCCGTTGACGCTGCTGCTGTCGTCGGTGGTGGGGGCG
CTGGTTGGCCTGTGCCTGCTGCGTTTGCGCCGGCATGCGATGGGCACGGCGATACCGTTTGGCCCTTATCTGGCGATTGC
GGGGTGGATTGCTGTGCTCTGGGGTGATGAAATGTACGCCTCATACATGCAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(137-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

54.348

95.833

0.521
  pilD Acinetobacter baumannii D1279779

50.534

97.569

0.493
  pilD Acinetobacter nosocomialis M2

49.822

97.569

0.486
  pilD Vibrio campbellii strain DS40M4

49.64

96.528

0.479
  pilD Neisseria gonorrhoeae MS11

49.814

93.403

0.465

Multiple sequence alignment