Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   B723_RS00590 Genome accession   NZ_CP010945
Coordinates   121949..123316 (-) Length   455 a.a.
NCBI ID   WP_008040321.1    Uniprot ID   J3H8S5
Organism   Pseudomonas fluorescens NCIMB 11764     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 116949..128316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B723_RS00565 (B723_00565) - 117218..117907 (+) 690 WP_017340879.1 GntR family transcriptional regulator -
  B723_RS00570 (B723_00570) yjiA 117961..118932 (-) 972 WP_017340880.1 GTPase -
  B723_RS00575 (B723_00575) - 119096..119293 (-) 198 WP_008052036.1 YbdD/YjiX family protein -
  B723_RS00580 (B723_00580) - 119308..121374 (-) 2067 WP_017340881.1 carbon starvation CstA family protein -
  B723_RS00585 (B723_00585) - 121533..121901 (+) 369 WP_017340882.1 PilZ domain-containing protein -
  B723_RS00590 (B723_00590) radA 121949..123316 (-) 1368 WP_008040321.1 DNA repair protein RadA Machinery gene
  B723_RS00595 (B723_00595) mscL 123952..124368 (+) 417 WP_017340883.1 large-conductance mechanosensitive channel protein MscL -
  B723_RS00600 (B723_00600) - 124410..125186 (-) 777 WP_017340884.1 ferredoxin--NADP reductase -
  B723_RS00605 (B723_00605) - 125527..126258 (+) 732 WP_017340885.1 LuxR family transcriptional regulator -
  B723_RS00610 (B723_00610) - 126420..127544 (+) 1125 WP_017340886.1 methyltransferase -
  B723_RS31785 - 127623..127790 (-) 168 WP_080995174.1 DUF2474 domain-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48732.20 Da        Isoelectric Point: 6.8987

>NTDB_id=140791 B723_RS00590 WP_008040321.1 121949..123316(-) (radA) [Pseudomonas fluorescens NCIMB 11764]
MAKAKRMYGCTECGSTFPKWAGQCGECGAWNTLTETMVESGGAAAPSGRTGWTGQQAQIKTLAEVSVEEIPRFSTASSEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLAKSMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARVEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=140791 B723_RS00590 WP_008040321.1 121949..123316(-) (radA) [Pseudomonas fluorescens NCIMB 11764]
ATGGCCAAGGCCAAACGCATGTACGGCTGCACCGAGTGCGGCTCAACCTTCCCCAAGTGGGCCGGCCAGTGCGGCGAGTG
CGGGGCCTGGAACACCTTGACCGAAACCATGGTCGAGAGCGGCGGGGCTGCGGCCCCCAGCGGTCGCACCGGCTGGACCG
GCCAACAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTCGAGGAAATACCGCGCTTTTCCACCGCGTCCAGTGAACTG
GACCGGGTCTTGGGTGGCGGTCTGGTGGACGGTTCGGTGGTGCTGATCGGCGGTGACCCCGGCATCGGCAAATCAACCAT
TCTTTTGCAAACCTTGTGCAACCTCGCCAAAAGCATGCCGGCGCTGTATGTCACCGGCGAAGAGTCCCAGCAACAGGTGG
CCATGCGCGCCCGGCGTCTTGGATTGCCGCAGGATCAACTGCGCGTCATGACCGAAACCTGCATCGAAACCATCATCGCC
ACTGCCCGGGTCGAAAAGCCCAAGGTCATGGTGATCGACTCGATTCAGACGATCTTCACCGAGCAACTGCAATCGGCACC
GGGCGGCGTGTCCCAGGTTCGGGAGAGCGCGGCATTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATTTTTCTGG
TTGGCCACGTGACGAAAGAAGGTGCGCTGGCCGGTCCGCGCGTGCTGGAGCACATGGTCGATACCGTGCTGTATTTCGAG
GGTGAATCCGACGGTCGCTTGCGCTTGTTGCGGGCGGTGAAGAACCGTTTCGGTGCAGTCAACGAACTGGGCGTGTTCGG
CATGACCGACAAGGGCTTGAAGGAAGTGTCCAATCCGTCGGCGATTTTTCTGACCCGTGCTCAGGAAGAAGTACCGGGCA
GTGTGGTCATGGCGACGTGGGAAGGGACCCGACCGATGCTGGTGGAAGTCCAGGCGCTGGTGGATGACAGCCATTTGGCC
AACCCGCGCCGCGTCACGCTGGGACTGGATCAGAACCGGCTGGCGATGTTGCTGGCAGTACTGCACCGCCATGGCGGCAT
TCCGACCCACGATCAGGACGTGTTCCTCAACGTGGTGGGTGGCGTGAAGGTGTTGGAAACCGCTTCGGACCTGGCGCTGA
TGGCGGCGGTCATGTCCAGTTTGCGCAACCGGCCACTGCCCCATGATCTGCTGGTGTTCGGTGAAGTCGGTTTGTCTGGC
GAAGTGCGCCCGGTGCCCAGCGGTCAGGAGCGTTTGAAGGAAGCCGCCAAGCACGGCTTCAAACGGGCCATTGTGCCCAA
GGGCAACGCGCCGAAAGAGGCGCCGCCGGGGTTGCAGATTATTGCCGTGACGCGTCTGGAACAGGCTCTCGACGCATTGT
TCGAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(74-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J3H8S5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.015

100

0.492
  radA Streptococcus pneumoniae Rx1

46.522

100

0.47
  radA Streptococcus pneumoniae R6

46.522

100

0.47
  radA Streptococcus pneumoniae TIGR4

46.522

100

0.47
  radA Streptococcus pneumoniae D39

46.522

100

0.47
  radA Streptococcus mitis SK321

46.711

100

0.468
  radA Streptococcus mitis NCTC 12261

46.491

100

0.466

Multiple sequence alignment