Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC22_RS13410 Genome accession   NZ_CP013248
Coordinates   2767326..2767778 (+) Length   150 a.a.
NCBI ID   WP_071652389.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_022     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2762326..2772778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC22_RS13395 (FORC22_2560) pdhR 2764358..2765125 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FORC22_RS13400 (FORC22_2561) ampD 2765531..2766082 (-) 552 WP_005484832.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC22_RS13405 (FORC22_2562) nadC 2766175..2767062 (+) 888 WP_071652388.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC22_RS13410 (FORC22_2563) pilA 2767326..2767778 (+) 453 WP_071652389.1 pilin Machinery gene
  FORC22_RS13415 (FORC22_2564) pilB 2767780..2769465 (+) 1686 WP_071652390.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC22_RS13420 (FORC22_2565) pilC 2769489..2770712 (+) 1224 WP_025523095.1 type II secretion system F family protein Machinery gene
  FORC22_RS13425 (FORC22_2566) pilD 2770777..2771646 (+) 870 WP_071652391.1 prepilin peptidase Machinery gene
  FORC22_RS13430 (FORC22_2567) coaE 2771647..2772261 (+) 615 WP_005480887.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 15684.97 Da        Isoelectric Point: 7.8142

>NTDB_id=139904 FORC22_RS13410 WP_071652389.1 2767326..2767778(+) (pilA) [Vibrio parahaemolyticus strain FORC_022]
MKNSKQKKQQGFTLIELMIVVGIIGIISALAVPAYKSYVLKTEANTAVGVPRALLANVDLFVQEKGKYPNSTQTADLAAI
GAAIDMSAMGTLAITPDADGSEYGDIEFTIGSNASLSGKKVTFARSTNGWKCTHDTGQDLKGCATTPATP

Nucleotide


Download         Length: 453 bp        

>NTDB_id=139904 FORC22_RS13410 WP_071652389.1 2767326..2767778(+) (pilA) [Vibrio parahaemolyticus strain FORC_022]
ATGAAAAACAGTAAACAGAAAAAACAGCAAGGTTTTACCTTGATTGAATTGATGATTGTGGTTGGGATTATTGGGATTAT
AAGTGCATTAGCTGTACCAGCTTATAAAAGCTATGTACTAAAAACCGAAGCTAATACTGCTGTGGGCGTGCCAAGAGCCT
TGTTAGCAAACGTAGACCTCTTCGTCCAAGAAAAAGGTAAATACCCTAATTCTACTCAAACTGCCGATCTTGCAGCTATC
GGAGCTGCTATAGATATGAGTGCGATGGGAACTCTTGCTATCACTCCTGACGCAGACGGTTCTGAGTACGGGGACATTGA
GTTTACCATTGGCTCTAATGCTTCATTGAGTGGCAAAAAGGTTACGTTTGCTCGCTCAACTAACGGTTGGAAGTGTACGC
ATGATACAGGTCAAGACCTTAAAGGCTGTGCTACCACTCCAGCAACTCCTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(56-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

37.838

98.667

0.373
  pilA Vibrio parahaemolyticus RIMD 2210633

41.353

88.667

0.367