Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RU53_RS08685 Genome accession   NZ_CP010526
Coordinates   1739803..1740489 (-) Length   228 a.a.
NCBI ID   WP_001788928.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1734803..1745489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RU53_RS08665 (RU53_1684) rpsT 1735717..1735968 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  RU53_RS08670 (RU53_1685) holA 1736013..1736987 (-) 975 WP_001282567.1 DNA polymerase III subunit delta -
  RU53_RS08675 (RU53_1686) comEC 1737044..1739245 (-) 2202 WP_001837660.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RU53_RS08680 (RU53_1687) - 1739250..1739711 (-) 462 WP_000439693.1 ComE operon protein 2 -
  RU53_RS08685 (RU53_1688) comEA 1739803..1740489 (-) 687 WP_001788928.1 ComEA family DNA-binding protein Machinery gene
  RU53_RS08690 (RU53_1689) - 1740529..1741245 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  RU53_RS08695 (RU53_1690) rsfS 1741248..1741601 (-) 354 WP_001088020.1 ribosome silencing factor -
  RU53_RS08700 (RU53_1691) yqeK 1741602..1742186 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RU53_RS08705 (RU53_1692) nadD 1742176..1742745 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  RU53_RS08710 (RU53_1693) yhbY 1742748..1743038 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  RU53_RS08715 (RU53_1694) aroE 1743042..1743848 (-) 807 WP_000666748.1 shikimate dehydrogenase -
  RU53_RS08720 (RU53_1695) yqeH 1743862..1744962 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25888.59 Da        Isoelectric Point: 9.6751

>NTDB_id=139083 RU53_RS08685 WP_001788928.1 1739803..1740489(-) (comEA) [Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=139083 RU53_RS08685 WP_001788928.1 1739803..1740489(-) (comEA) [Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

100

99.561

0.996
  comEA Staphylococcus aureus MW2

97.357

99.561

0.969

Multiple sequence alignment