Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACT75_RS07350 Genome accession   NZ_CP012959
Coordinates   1508079..1508768 (-) Length   229 a.a.
NCBI ID   WP_005594882.1    Uniprot ID   Q8KRY3
Organism   Aggregatibacter actinomycetemcomitans strain 624     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1503079..1513768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACT75_RS12525 - 1503233..1503409 (-) 177 WP_014702332.1 hypothetical protein -
  ACT75_RS07330 (ACT75_07320) - 1503418..1504428 (-) 1011 WP_061866559.1 WD40 repeat domain-containing protein -
  ACT75_RS07335 (ACT75_07325) - 1504431..1506551 (-) 2121 WP_005539198.1 alpha/beta hydrolase -
  ACT75_RS07340 (ACT75_07330) - 1506574..1507701 (-) 1128 WP_005539200.1 DUF4123 domain-containing protein -
  ACT75_RS07350 (ACT75_07340) pilD 1508079..1508768 (-) 690 WP_005594882.1 prepilin peptidase Machinery gene
  ACT75_RS07355 (ACT75_07345) pilC 1508765..1509988 (-) 1224 WP_061866560.1 type II secretion system F family protein Machinery gene
  ACT75_RS07360 (ACT75_07350) pilB 1509981..1511390 (-) 1410 WP_005540457.1 GspE/PulE family protein Machinery gene
  ACT75_RS07365 (ACT75_07355) pilA 1511417..1511869 (-) 453 WP_005550263.1 pilin Machinery gene
  ACT75_RS07370 (ACT75_07360) ampD 1511991..1512545 (+) 555 WP_005540460.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACT75_RS07375 (ACT75_07365) nanQ 1513011..1513484 (-) 474 WP_005594879.1 N-acetylneuraminate anomerase -

Sequence

Protein


Download         Length: 229 a.a.        Molecular weight: 26056.79 Da        Isoelectric Point: 7.9832

>NTDB_id=137747 ACT75_RS07350 WP_005594882.1 1508079..1508768(-) (pilD) [Aggregatibacter actinomycetemcomitans strain 624]
MMWLLFLLGGVLCGWRVYHYVEHFADNLSREVHAAYTEIYPQNPPHFTAENSVLKPLKRHFSAHLYSLLFTLLFMICYAI
NEPLQALCFALYGALLIVISLVDWHYRLISPALCQALFALGLGAAYWQLGTLTLAQSLQSAVIFFGVFYAIYHAAKWYYR
QEALGRGDYWLALGLGVFLPAQQLPVFLFLACFFGLLYAAYAKYQHRTLNTMPFGPFMSLAGVVCLLLN

Nucleotide


Download         Length: 690 bp        

>NTDB_id=137747 ACT75_RS07350 WP_005594882.1 1508079..1508768(-) (pilD) [Aggregatibacter actinomycetemcomitans strain 624]
ATGATGTGGCTACTTTTTCTGCTTGGCGGTGTGCTTTGTGGCTGGCGCGTTTATCATTACGTAGAACATTTCGCCGATAA
CCTCAGCCGCGAGGTTCATGCCGCCTACACGGAAATTTACCCGCAAAACCCACCGCACTTTACGGCGGAAAACTCGGTTC
TAAAACCGCTTAAACGGCATTTTTCCGCACATCTTTACAGCCTTTTATTCACCTTGCTGTTCATGATTTGTTATGCCATC
AATGAACCGTTACAAGCCCTTTGTTTTGCACTGTATGGCGCCTTATTAATCGTCATTTCTTTGGTGGATTGGCACTATCG
CCTGATTTCACCCGCCTTATGCCAAGCCTTGTTCGCTCTCGGTTTAGGCGCGGCATACTGGCAACTCGGCACATTAACAT
TGGCGCAAAGCCTGCAAAGTGCGGTCATTTTTTTCGGTGTTTTTTATGCCATCTACCACGCCGCAAAATGGTACTACCGA
CAAGAAGCTCTCGGACGCGGCGATTATTGGCTGGCACTGGGCTTAGGCGTTTTTCTACCGGCACAACAACTTCCCGTTTT
TTTATTCCTCGCCTGCTTTTTCGGCTTACTTTATGCCGCCTACGCAAAATACCAACACCGCACACTCAACACCATGCCTT
TCGGTCCCTTTATGTCCCTAGCCGGCGTGGTCTGTTTATTGCTTAATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(94-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8KRY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

38.865

100

0.389
  pilD Haemophilus influenzae Rd KW20

38.393

97.817

0.376

Multiple sequence alignment