Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SD89_RS02640 Genome accession   NZ_CP010449
Coordinates   517167..518492 (+) Length   441 a.a.
NCBI ID   WP_053308256.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NGAS322     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 512167..523492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD89_RS02615 (SD89_02610) - 512938..513579 (+) 642 WP_002983671.1 response regulator transcription factor -
  SD89_RS02620 (SD89_02615) - 513616..515016 (+) 1401 WP_053308255.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SD89_RS02625 (SD89_02620) - 515016..515393 (+) 378 WP_032464495.1 S1 RNA-binding domain-containing protein -
  SD89_RS02630 (SD89_02625) cysK 515411..516352 (-) 942 WP_011528853.1 cysteine synthase A -
  SD89_RS02635 (SD89_02630) - 516480..517112 (-) 633 WP_002983676.1 YigZ family protein -
  SD89_RS02640 (SD89_02635) comFA/cflA 517167..518492 (+) 1326 WP_053308256.1 DEAD/DEAH box helicase Machinery gene
  SD89_RS02645 (SD89_02640) - 518464..519129 (+) 666 WP_053308257.1 ComF family protein -
  SD89_RS02650 (SD89_02645) hpf 519209..519757 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49757.20 Da        Isoelectric Point: 10.0339

>NTDB_id=137331 SD89_RS02640 WP_053308256.1 517167..518492(+) (comFA/cflA) [Streptococcus pyogenes strain NGAS322]
MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGNPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSSMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=137331 SD89_RS02640 WP_053308256.1 517167..518492(+) (comFA/cflA) [Streptococcus pyogenes strain NGAS322]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAGGCAACTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAGTTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCAGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCAATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTAGTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae D39

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae R6

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae TIGR4

54.177

95.011

0.515
  comFA/cflA Streptococcus mitis NCTC 12261

52.844

95.692

0.506
  comFA/cflA Streptococcus mitis SK321

51.896

95.692

0.497
  comFA Lactococcus lactis subsp. cremoris KW2

47.368

90.476

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365

Multiple sequence alignment