Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS09870   Type   Unclear
Locus tag   RA0C_RS09870 Genome accession   NC_017045
Coordinates   2096402..2097163 (+) Length   253 a.a.
NCBI ID   WP_013447186.1    Uniprot ID   E4TDQ0
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

RA0C_RS09855, RA0C_RS09870, RA0C_RS09875, RA0C_RS09880, RA0C_RS03780, RA0C_RS07335, RA0C_RS08100, RA0C_RS04070, RA0C_RS07455, RA0C_RS05975, RA0C_RS06070, and RA0C_RS08490 significantly affected the natural transformation frequency of RA.


Genomic Context

Location: 2091402..2102163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS09850 (RA0C_2046) - 2092718..2093626 (+) 909 WP_013447182.1 putative zinc-binding metallopeptidase -
  RA0C_RS09855 (RA0C_2047) RA0C_RS09855 2093633..2094544 (+) 912 WP_013447183.1 DUF4302 domain-containing protein Unclear
  RA0C_RS09860 (RA0C_2048) - 2094559..2095167 (+) 609 WP_013447184.1 hypothetical protein -
  RA0C_RS09865 (RA0C_2049) - 2095185..2096366 (+) 1182 WP_013447185.1 DUF4856 domain-containing protein -
  RA0C_RS09870 (RA0C_2050) RA0C_RS09870 2096402..2097163 (+) 762 WP_013447186.1 T9SS type A sorting domain-containing protein Unclear
  RA0C_RS09875 (RA0C_2051) RA0C_RS09875 2097174..2098337 (+) 1164 WP_013447187.1 imelysin family protein Unclear
  RA0C_RS09880 (RA0C_2052) RA0C_RS09880 2098327..2099700 (+) 1374 WP_013447188.1 HTTM domain-containing protein Unclear
  RA0C_RS09885 (RA0C_2053) - 2099681..2101894 (+) 2214 WP_013447189.1 TonB-dependent receptor family protein -

Sequence

Protein


Download         Length: 253 a.a.        Molecular weight: 28464.09 Da        Isoelectric Point: 9.4262

>NTDB_id=1337 RA0C_RS09870 WP_013447186.1 2096402..2097163(+) (RA0C_RS09870) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MKKFLLTIGVLSSSLLFSQNLITNSSLEQWSSSCYLGNCRYYPKDWKGNAKQSAENPKEGTYSAELGSGRNIEFELRQGI
EAGQKYILSYYVYSTGAERQLVHRFRWRDANRTTIATNGDDYTHSVTTFGQWEKVEKEFVAPGGAAGFYINLNTLGSGSE
VIRLDDFKFYKQGSLGTQEWSSQKEVQLHTQGKELQVFTTKPVTIKVYGVGGQLYVTKTVNSNDRITLSALPHGIYLVLV
ETDKGTFSKKIRL

Nucleotide


Download         Length: 762 bp        

>NTDB_id=1337 RA0C_RS09870 WP_013447186.1 2096402..2097163(+) (RA0C_RS09870) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGAAAAAGTTTTTACTAACCATAGGGGTACTAAGCTCCTCGTTATTATTTTCTCAAAACCTGATAACCAACTCAAGTCT
GGAGCAATGGAGTAGTAGTTGCTATTTAGGGAATTGCAGATATTATCCTAAAGATTGGAAAGGTAATGCTAAACAATCAG
CGGAAAATCCGAAAGAGGGAACATATAGTGCAGAGTTAGGTTCTGGTAGGAATATAGAATTTGAGTTGAGACAAGGGATA
GAAGCAGGTCAGAAATATATTTTAAGTTATTATGTGTATTCTACAGGTGCAGAAAGACAATTGGTTCATCGTTTCAGATG
GAGAGATGCGAATAGGACAACTATAGCAACAAACGGAGATGACTATACTCATTCTGTAACGACTTTCGGACAGTGGGAAA
AAGTAGAAAAAGAGTTTGTTGCCCCAGGGGGTGCAGCAGGATTTTACATTAACCTCAATACTTTGGGGAGTGGCTCGGAA
GTCATCCGATTAGACGATTTCAAATTTTATAAGCAGGGAAGTTTGGGAACACAAGAGTGGTCTTCGCAAAAAGAAGTACA
GCTCCATACCCAAGGAAAGGAACTCCAAGTTTTTACAACCAAACCCGTAACGATTAAAGTATATGGCGTTGGAGGACAAT
TATATGTTACAAAAACGGTGAACTCCAATGATAGGATAACTTTGTCGGCTTTACCTCACGGTATTTATTTGGTGTTGGTA
GAAACAGATAAAGGGACATTCAGTAAAAAAATAAGATTATAA

Domains

Predicted by InterproScan.

Secretion system C-terminal sorting domain (Por_Secre_tail)

(199-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TDQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]