Detailed information    

experimental Experimentally validated

Overview

Name   RA0C_RS09855   Type   Unclear
Locus tag   RA0C_RS09855 Genome accession   NC_017045
Coordinates   2093633..2094544 (+) Length   303 a.a.
NCBI ID   WP_013447183.1    Uniprot ID   E4TDP7
Organism   Riemerella anatipestifer ATCC 11845 = DSM 15868     
Function   require for natural transformation   
Unclear

Function

RA0C_RS09855, RA0C_RS09870, RA0C_RS09875, RA0C_RS09880, RA0C_RS03780, RA0C_RS07335, RA0C_RS08100, RA0C_RS04070, RA0C_RS07455, RA0C_RS05975, RA0C_RS06070, and RA0C_RS08490 significantly affected the natural transformation frequency of RA.


Genomic Context

Location: 2088633..2099544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA0C_RS09845 (RA0C_2045) - 2091237..2092709 (+) 1473 WP_013447181.1 RagB/SusD family nutrient uptake outer membrane protein -
  RA0C_RS09850 (RA0C_2046) - 2092718..2093626 (+) 909 WP_013447182.1 putative zinc-binding metallopeptidase -
  RA0C_RS09855 (RA0C_2047) RA0C_RS09855 2093633..2094544 (+) 912 WP_013447183.1 DUF4302 domain-containing protein Unclear
  RA0C_RS09860 (RA0C_2048) - 2094559..2095167 (+) 609 WP_013447184.1 hypothetical protein -
  RA0C_RS09865 (RA0C_2049) - 2095185..2096366 (+) 1182 WP_013447185.1 DUF4856 domain-containing protein -
  RA0C_RS09870 (RA0C_2050) RA0C_RS09870 2096402..2097163 (+) 762 WP_013447186.1 T9SS type A sorting domain-containing protein Unclear
  RA0C_RS09875 (RA0C_2051) RA0C_RS09875 2097174..2098337 (+) 1164 WP_013447187.1 imelysin family protein Unclear

Sequence

Protein


Download         Length: 303 a.a.        Molecular weight: 35331.86 Da        Isoelectric Point: 8.4893

>NTDB_id=1336 RA0C_RS09855 WP_013447183.1 2093633..2094544(+) (RA0C_RS09855) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
MKNTIIVFVSLFLLVGACRDKDETIFSETPSQRTQEHIAALRNELVSAEEGWWFTYFPDVDSLRFSDPNKNIKTSDNAIV
FLERQFGQGGYTFYMKFNEKGTVEMLSDTDYNAANNLKMSDFEVKQSSYTQLSFTTYNYIHQLNKSDFLFWKKDSEGNLI
FRTNRYLDTNREYLVMKKAILNGKQASDRMNLMYQNKINYERLYNPILTIKDSEGGVLFQSNLPYKKQDVKNRYQAFVKN
WQPNLYNSSYYSGVASGYVATQEGLFFYPGIQLTDQTKFRVFTKEGEAYKSVVNGYEALITIK

Nucleotide


Download         Length: 912 bp        

>NTDB_id=1336 RA0C_RS09855 WP_013447183.1 2093633..2094544(+) (RA0C_RS09855) [Riemerella anatipestifer ATCC 11845 = DSM 15868]
ATGAAAAACACGATAATCGTCTTTGTAAGTTTGTTTCTATTAGTGGGAGCTTGTAGGGATAAAGATGAAACTATTTTCAG
CGAAACACCTTCTCAAAGAACTCAAGAGCATATCGCTGCATTGAGAAATGAGTTGGTTTCAGCCGAAGAAGGGTGGTGGT
TTACCTATTTTCCAGACGTAGATTCTTTGCGTTTTTCAGACCCGAATAAAAATATTAAAACGAGTGATAACGCCATTGTC
TTTTTAGAAAGACAATTCGGGCAAGGAGGCTATACATTCTATATGAAATTTAATGAAAAAGGAACGGTAGAAATGCTAAG
CGATACCGATTATAACGCTGCAAATAACCTCAAAATGAGTGATTTTGAAGTGAAACAAAGCTCCTACACTCAACTTAGTT
TTACCACTTATAATTACATACACCAACTGAATAAAAGTGATTTTCTATTTTGGAAAAAAGATAGTGAGGGCAACTTAATA
TTCCGTACCAACCGTTATTTGGATACCAACAGAGAATATTTGGTAATGAAGAAAGCCATACTTAACGGAAAACAAGCATC
GGATAGAATGAATTTGATGTATCAAAACAAAATAAATTACGAGAGACTCTACAATCCTATTTTAACGATAAAAGATTCGG
AGGGCGGTGTTTTGTTTCAAAGCAATTTACCTTATAAAAAGCAAGATGTAAAGAATAGATACCAAGCCTTTGTTAAGAAC
TGGCAACCTAATCTTTACAATAGTTCTTATTATTCTGGGGTGGCTTCTGGTTATGTGGCTACGCAAGAGGGGTTGTTTTT
CTATCCAGGAATACAGCTTACAGACCAAACGAAATTTAGGGTTTTTACTAAAGAAGGTGAGGCTTACAAATCGGTGGTAA
ACGGTTACGAAGCCTTAATTACGATAAAATAA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4302) (DUF4302)

(22-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TDP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Li Huang et al. (2021) Identification of the Natural Transformation Genes in Riemerella anatipestifer by Random Transposon Mutagenesis. Frontiers in Microbiology 12:712198. [PMID: 34566918]