Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BF25_RS02800 Genome accession   NZ_CP009981
Coordinates   274372..276711 (+) Length   779 a.a.
NCBI ID   WP_000941254.1    Uniprot ID   A0A6H3AMP8
Organism   Bacillus anthracis strain Ames isolate BACI008     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 269372..281711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF25_RS02785 (BF25_293) - 270404..270688 (+) 285 WP_000886985.1 post-transcriptional regulator -
  BF25_RS02790 (BF25_294) secDF 270959..273223 (+) 2265 WP_001119062.1 protein translocase subunit SecDF -
  BF25_RS02795 (BF25_295) - 273377..274270 (+) 894 WP_000409725.1 cation diffusion facilitator family transporter -
  BF25_RS02800 (BF25_296) recJ 274372..276711 (+) 2340 WP_000941254.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BF25_RS02805 (BF25_297) - 276759..277271 (+) 513 WP_000346214.1 adenine phosphoribosyltransferase -
  BF25_RS02810 (BF25_298) relA 277482..279665 (+) 2184 WP_001262816.1 GTP diphosphokinase -
  BF25_RS02815 (BF25_299) - 279683..280123 (+) 441 WP_001266954.1 D-tyrosyl-tRNA(Tyr) deacylase -
  BF25_RS02820 (BF25_300) - 280309..280485 (+) 177 WP_001242605.1 hypothetical protein -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88181.80 Da        Isoelectric Point: 4.9388

>NTDB_id=133357 BF25_RS02800 WP_000941254.1 274372..276711(+) (recJ) [Bacillus anthracis strain Ames isolate BACI008]
MLQPKTRWKEKEYNGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATLAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEEILTEEDFIPITAVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVK
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSKEVLHKFSLEDYKEHMMHASEVTELDEQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTRDHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=133357 BF25_RS02800 WP_000941254.1 274372..276711(+) (recJ) [Bacillus anthracis strain Ames isolate BACI008]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGGCGAGCGAGTGAGTGAATTAGCAAGTAAATTACAATT
ATCACCACTTGTCGTTTCGTTATTCCTCGGTAGAGGATTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGATGCGGACGGAGTAAGTAGTACGACTGTGTTATATCTTGCCTTGCAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTTCGTTGGGCGC
ATAGCGCAGGATTCTCATTAATTATTACTGTCGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTAATTATTACAGATCACCATGAGCCGCCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCACTTCACGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATTGGACTAAAGGCGCTATTTAAAGTAGCTAATGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
TGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGCTGGCTGAAGAAATTGATGAACTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAGGTGGAAAATAATTTCCCGCCTGAAGAAAATAAAGT
ATTAGTGCTTGCAAAAGAAGGCTGGAATCCAGGTGTAATTGGAATTGTCGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGGGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAGCAAGCAGAGGAAATTTTAACGGAAGAAGATTTTATCCCAATTACAG
CTGTCGATGCTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCAATCGAGGATATGCAAAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCAATCGGATCAGACGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAGGCAACACTGGATACAATCGGGTTTGGATTTGGAGCGTATGCGAAAGAAATTTCTC
CAGTTGCAAAAGTATCTGTAATCGGGGAAGCGTCTATTAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTGAAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAAGCAAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTAAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACCGAGTTAGATGAGCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGATCACTTTAAATGGTACTATTCATTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAGTTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGCTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H3AMP8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.842

99.358

0.525

Multiple sequence alignment