Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PN96_RS01335 Genome accession   NZ_CP009977
Coordinates   255293..256516 (-) Length   407 a.a.
NCBI ID   WP_020333949.1    Uniprot ID   -
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 250293..261516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN96_RS01295 (PN96_01265) rpsP 250371..250619 (+) 249 WP_005379962.1 30S ribosomal protein S16 -
  PN96_RS01300 (PN96_01270) rimM 250650..251198 (+) 549 WP_014232958.1 ribosome maturation factor RimM -
  PN96_RS01305 (PN96_01275) trmD 251226..251987 (+) 762 WP_014232957.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PN96_RS01310 (PN96_01280) rplS 252028..252381 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  PN96_RS01315 (PN96_01285) yacG 252706..252900 (-) 195 WP_014232956.1 DNA gyrase inhibitor YacG -
  PN96_RS01320 (PN96_01290) zapD 252974..253714 (-) 741 WP_020333952.1 cell division protein ZapD -
  PN96_RS01325 (PN96_01295) coaE 253741..254355 (-) 615 WP_020333951.1 dephospho-CoA kinase -
  PN96_RS01330 (PN96_01300) pilD 254356..255225 (-) 870 WP_020333950.1 A24 family peptidase Machinery gene
  PN96_RS01335 (PN96_01305) pilC 255293..256516 (-) 1224 WP_020333949.1 type II secretion system F family protein Machinery gene
  PN96_RS01340 (PN96_01310) pilB 256547..258232 (-) 1686 WP_020333948.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PN96_RS01345 (PN96_01315) pilA 258233..258691 (-) 459 WP_020333947.1 pilin Machinery gene
  PN96_RS01350 (PN96_01320) nadC 258958..259845 (-) 888 WP_020333946.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PN96_RS01355 (PN96_01325) ampD 259938..260483 (+) 546 WP_020333945.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45051.28 Da        Isoelectric Point: 10.3231

>NTDB_id=133317 PN96_RS01335 WP_020333949.1 255293..256516(-) (pilC) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MKTTTPQLKNFRWKGINSSGKKTSGQTLAMSEVEVRERLSAQHIKIKKLKKSSISFITKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSNNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASAHFDALYTDLISTGELSGNLAEVFERLATYREK
SEELRAKVIKALIYPAMVMLVALGVSYLMLTQVIPEFEKMFSGFGADLPWFTRQVLDLSAWMQNWGSFIALGALSLIISA
RILVKRSDSFRLMMDRAILRIPVLGAVLSKAAIAKFSRTLATSFSAGIPILTSLKTTSKTSGNLHYQLAVEEVYRDTAAG
MPMYVAMRNCDVFPELVLQMVMIGEESGRLDEMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=133317 PN96_RS01335 WP_020333949.1 255293..256516(-) (pilC) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGAAAACGACGACCCCACAACTAAAGAACTTCCGCTGGAAAGGCATTAATAGCTCAGGAAAAAAAACTTCTGGGCAAAC
GCTGGCCATGAGCGAAGTGGAAGTACGCGAACGACTCAGCGCTCAGCACATAAAAATCAAAAAACTGAAAAAAAGTAGTA
TTTCTTTTATAACCAAACTGAGTCATCGGGTAAAAGGCAAAGACATCACCGTTTTTACTCGTCAAATTTCGACTATGCTG
GTCACTGGTGTCCCACTCGTTCAGGCTTTAAAATTGGTTTCCAATAACCATAAAAAAGCCGAGATGAAGTCTATTTTAAT
GAGCGTAACTCGAGCGGTTGAAGCGGGTACACCGATGTCGAAAGCAATGCGCACCGCCAGCGCACATTTTGACGCACTTT
ATACTGACCTTATCTCAACCGGAGAACTCTCAGGCAATCTGGCAGAAGTCTTCGAACGACTGGCCACTTACCGTGAAAAA
AGTGAGGAACTCAGAGCGAAAGTCATCAAAGCCCTGATCTACCCAGCCATGGTTATGTTGGTTGCGCTCGGCGTGTCCTA
TCTAATGCTCACTCAAGTTATCCCTGAGTTTGAAAAAATGTTTTCTGGGTTTGGTGCAGATTTACCTTGGTTCACCAGAC
AAGTACTAGACTTGTCTGCGTGGATGCAAAATTGGGGTTCATTTATCGCTCTTGGGGCACTTAGCCTAATTATATCAGCA
AGAATACTGGTCAAACGTTCTGATTCTTTTCGCTTGATGATGGATCGAGCCATATTGAGAATTCCCGTTCTTGGCGCTGT
GTTGTCCAAGGCTGCGATTGCTAAATTTAGCCGAACCCTGGCCACCAGCTTTAGCGCGGGTATTCCTATTCTGACCAGTT
TAAAAACCACCTCCAAAACGTCAGGAAACCTGCACTATCAGCTGGCAGTCGAAGAAGTCTATCGTGATACAGCAGCTGGT
ATGCCAATGTATGTCGCAATGCGTAACTGCGATGTTTTCCCTGAATTAGTGCTACAAATGGTGATGATTGGAGAAGAATC
AGGCAGGCTTGATGAGATGCTCAATAAAGTCGCAACTATTTATGAGTTCGAAGTAGATAATACCGTCGATAACCTAAGTA
AGATTCTTGAACCGCTGATTATTGTTTTTCTTGGTGTGGTCGTTGGTGGATTAGTGACAGCAATGTACCTGCCAATCTTT
AACTTAATGAGTGTATTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.749

100

0.857
  pilC Vibrio cholerae strain A1552

73.333

99.509

0.73
  pilC Acinetobacter baylyi ADP1

42

98.28

0.413
  pilC Legionella pneumophila strain ERS1305867

41.235

99.509

0.41
  pilG Neisseria gonorrhoeae MS11

40.447

99.017

0.4
  pilG Neisseria meningitidis 44/76-A

40.199

99.017

0.398
  pilC Acinetobacter baumannii D1279779

40.5

98.28

0.398
  pilC Pseudomonas stutzeri DSM 10701

40.399

98.526

0.398

Multiple sequence alignment