Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   BF28_RS24845 Genome accession   NZ_CP009968
Coordinates   4259043..4260869 (-) Length   608 a.a.
NCBI ID   WP_000003397.1    Uniprot ID   Q63EH1
Organism   Bacillus cereus E33L     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 4254043..4265869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BF28_RS24810 (BF28_4402) - 4254466..4255263 (-) 798 WP_000673194.1 NAD kinase -
  BF28_RS24815 (BF28_4403) - 4255282..4255920 (-) 639 WP_001081482.1 GTP pyrophosphokinase family protein -
  BF28_RS24820 (BF28_4404) - 4255951..4256322 (-) 372 WP_001180007.1 hypothetical protein -
  BF28_RS24825 (BF28_4405) - 4256469..4257047 (+) 579 WP_000191108.1 CYTH domain-containing protein -
  BF28_RS24830 (BF28_4406) - 4257228..4257626 (+) 399 WP_000043381.1 hypothetical protein -
  BF28_RS24835 (BF28_4407) - 4257626..4258519 (+) 894 WP_000360666.1 ClpXP adapter SpxH family protein -
  BF28_RS33200 (BF28_4408) - 4258749..4258922 (+) 174 WP_041184829.1 hypothetical protein -
  BF28_RS24845 (BF28_4409) pepF 4259043..4260869 (-) 1827 WP_000003397.1 oligoendopeptidase F Regulator
  BF28_RS24850 (BF28_4410) - 4260920..4262164 (-) 1245 WP_000612275.1 competence protein CoiA -
  BF28_RS24855 (BF28_4411) - 4262245..4263789 (-) 1545 WP_000799206.1 cardiolipin synthase -
  BF28_RS24860 (BF28_4412) mecA 4263862..4264545 (-) 684 WP_000350710.1 adaptor protein MecA Regulator
  BF28_RS24865 (BF28_4413) - 4264872..4265546 (+) 675 WP_000362618.1 TerC family protein -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70297.56 Da        Isoelectric Point: 4.6201

>NTDB_id=133242 BF28_RS24845 WP_000003397.1 4259043..4260869(-) (pepF) [Bacillus cereus E33L]
MSEQNTAKTLPDRNEIEESSTWRLEDIFQTDTEWEKEFQAIKELLPKLTEFKGKLGDSANNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSASSNTFGMLNNADLKFPSIKGEDGEEVEITHGRYIQFLESDDRRVREDAFKAVYETYGKYKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVESVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDMLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=133242 BF28_RS24845 WP_000003397.1 4259043..4260869(-) (pepF) [Bacillus cereus E33L]
ATGTCTGAACAAAATACAGCAAAAACATTACCAGATCGTAACGAGATTGAAGAATCAAGTACGTGGAGATTGGAAGATAT
TTTCCAAACGGATACAGAGTGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGAACAATTTACTTGAAGCATTGCAATATGAAGATGAAATTTCAATGCGATTAGGTAAGTTATAT
ACATATGCACATATGCGTTACGATCAAGATACAACAAACTCTGTGTATCAAGCGTTAAATGACCGCGCGACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCATATATTGTGCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTAA
AAGAAAATAGAGACTTAAGTGTATATGAACATGCATTAGAAGAAATTACGCGCCAACGTCCGCACGTATTATCTGAAGCG
GAAGAGGCTTTATTAGCAGAAGCATCTGAAGTAATGAGTGCGTCAAGTAATACATTTGGTATGTTGAATAATGCGGATTT
GAAATTCCCATCTATTAAAGGTGAGGATGGAGAAGAAGTAGAGATTACACATGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATCGTCGTGTGCGCGAAGATGCATTTAAAGCTGTATATGAAACGTACGGGAAATATAAGAATACATTTGCAAGTACG
TTAAGCGGAGCTGTGAAACGTAATAATTTTAATGCGCGCGTTCGTAAATACGACTCTGCACGTCAAGCGGCATTAAGTAA
TAATAATATTCCTGAAGCAGTGTACGATCAACTTGTTGAATCTGTAAATGATAATTTACACTTGTTACATCGTTACATTG
ATATTCGTAAGCGTGCACTAGGGCTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AACGTGAAGTATGAAGAAGCGCAAGACATGTTATTAAAGTCTTTAAACGTACTTGGTGATGAGTATGTTGATATTTTAAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGAGCATATTCATCTGGTGCGTATG
GAACAAATCCTTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAATTTGGGCATTCT
GTGCATAGTTACTACACAAGAAAAACGCAGCCGCACGTATACGGTGACTATTCAATCTTTGTTGCGGAAGTAGCATCAAC
TTGTAATGAAGCGCTGCTAAACGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATACTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTCGCAGAGTTTGAGCATATTATTCATAAGAAAGTACAA
GAGGGACATGCGGTTACGCCAGACATGTTAACGGAGATCTACTATGATTTAAATAAGAAATATTTCGGTGATGCTTTAGT
AATTGATGAAGAAATTGGTTTAGAGTGGTCACGTATTCCGCACTTTTACTACAACTATTACGTATACCAATACGCAACAG
GATTTAGTGCAGCAACTGCTCTATCTAAACAAATTTTAGAAGAAGGGCAACCAGCAGTAGAACGTTATATTAATGAGTTC
TTAAAAGCAGGAAGCTCGGATTACCCAATTGAAGTGCTGAAAAAAGCTGGAGTAGATATGGCATCTCCAGAACCGGTAAA
AGAAGCATTGCAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAGAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q63EH1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488

Multiple sequence alignment