Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC4_RS13750 Genome accession   NZ_CP009847
Coordinates   2989429..2990445 (-) Length   338 a.a.
NCBI ID   WP_043036352.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_004     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2984429..2995445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC4_RS13725 (FORC4_2621) aroK 2985289..2985807 (-) 519 WP_005381319.1 shikimate kinase AroK -
  FORC4_RS13730 (FORC4_2622) pilQ 2985996..2987744 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  FORC4_RS13735 (FORC4_2623) pilP 2987776..2988291 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  FORC4_RS13740 (FORC4_2624) pilO 2988281..2988874 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC4_RS13745 (FORC4_2625) pilN 2988867..2989445 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  FORC4_RS13750 (FORC4_2626) pilM 2989429..2990445 (-) 1017 WP_043036352.1 type IV pilus biogenesis protein PilM Machinery gene
  FORC4_RS13755 (FORC4_2627) - 2990588..2993158 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -
  FORC4_RS13760 (FORC4_2628) oxyR 2993214..2994122 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC4_RS13765 (FORC4_2629) - 2994293..2995021 (+) 729 WP_057619621.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37693.17 Da        Isoelectric Point: 6.6634

>NTDB_id=132572 FORC4_RS13750 WP_043036352.1 2989429..2990445(-) (pilM) [Vibrio parahaemolyticus strain FORC_004]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
SQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=132572 FORC4_RS13750 WP_043036352.1 2989429..2990445(-) (pilM) [Vibrio parahaemolyticus strain FORC_004]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATTTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
AGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482

Multiple sequence alignment