Detailed information NTG0132056

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: NC16_RS15205
• Genome accession: NZ_CP009769
• Coordinates: 3344634..3345479 (-)
• Length: 281 a.a.
• NCBI ID: WP_039503004.1
• Uniprot ID: -
• Organism: Pectobacterium brasiliense strain BC1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3339634..3350479

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NC16_RS15185 (NC16_14620)	-	3339836..3340606 (-)	771	WP_039502998.1	ABC transporter ATP-binding protein	-
NC16_RS15190 (NC16_14625)	-	3340608..3341645 (-)	1038	WP_080724594.1	FecCD family ABC transporter permease	-
NC16_RS15195 (NC16_14630)	-	3341642..3342706 (-)	1065	WP_039503000.1	ABC transporter substrate-binding protein	-
NC16_RS15200 (NC16_14635)	-	3343016..3344041 (+)	1026	WP_039503002.1	LLM class flavin-dependent oxidoreductase	-
NC16_RS22710	ypdK	3344399..3344473 (+)	75	WP_099606229.1	membrane protein YpdK	-
NC16_RS15205 (NC16_14640)	pilD	3344634..3345479 (-)	846	WP_039503004.1	prepilin peptidase	Machinery gene
NC16_RS15210 (NC16_14645)	-	3345588..3346334 (-)	747	WP_039503007.1	type II secretion system protein N	-
NC16_RS15215 (NC16_14650)	gspM	3346324..3346818 (-)	495	WP_039503010.1	type II secretion system protein GspM	-
NC16_RS15220 (NC16_14655)	gspL	3346815..3348092 (-)	1278	WP_039503012.1	type II secretion system protein GspL	-
NC16_RS15225 (NC16_14660)	gspK	3348089..3349075 (-)	987	WP_014916095.1	type II secretion system minor pseudopilin GspK	-
NC16_RS15230 (NC16_14665)	gspJ	3349072..3349722 (-)	651	WP_072035873.1	type II secretion system minor pseudopilin GspJ	-
NC16_RS15235 (NC16_14670)	gspI	3349722..3350087 (-)	366	WP_039284733.1	type II secretion system minor pseudopilin GspI	-

Sequence

Protein

• Length: 281 a.a.
• Molecular weight: 31426.39 Da
• Isoelectric Point: 5.7656

>NTDB_id=132056 NC16_RS15205 WP_039503004.1 3344634..3345479(-) (pilD) [Pectobacterium brasiliense strain BC1]
MDDLREFAQLFPAWWFGSLGVLGLIVGSFLNVVIYRLPIMLERRWRQDIELETGMVDSDPDTRYNLWWPPSSCPHCQQAI
AVKDNIPLFSWLWLRGRSRCCHQSVSVQYPLVEVITMLAFLTAGLLWLPGMALWGALILLSFLLVLTVIDIKTLLLPDVL
TLSLLWIGLLFNLSETFVPLSDAVVGAMAGYLSLWLLYWVFKYATGKEALGYGDFKLLAALGAWLGWQALPNLVLVAALS
GLVVTLIWRGLRKEDTAKPLAFGPWLAMGGVFGVIMNGLNL

Nucleotide

• Length: 846 bp

>NTDB_id=132056 NC16_RS15205 WP_039503004.1 3344634..3345479(-) (pilD) [Pectobacterium brasiliense strain BC1]
GTGGACGATTTAAGGGAATTTGCACAGCTGTTTCCGGCGTGGTGGTTTGGATCGCTGGGCGTGCTGGGATTGATTGTTGG
CAGTTTTCTGAACGTGGTGATTTACCGCTTGCCGATTATGCTGGAGCGTCGCTGGCGGCAGGACATCGAGCTTGAAACGG
GCATGGTCGATTCCGATCCTGATACGCGCTACAACCTGTGGTGGCCGCCTTCGTCGTGCCCGCACTGCCAGCAGGCTATT
GCGGTGAAGGATAACATCCCGCTATTCAGTTGGCTGTGGTTGCGTGGCCGCTCTCGCTGTTGTCATCAATCGGTGTCTGT
GCAATATCCGCTAGTGGAAGTCATCACCATGCTGGCTTTTCTGACGGCAGGTTTACTCTGGTTGCCCGGTATGGCGCTGT
GGGGGGCGTTGATTCTGCTGTCCTTCCTGTTGGTGCTGACCGTTATTGATATAAAAACGCTGCTGCTGCCGGATGTGCTT
ACGCTGTCGCTTCTGTGGATAGGGCTGCTGTTTAATCTGTCGGAGACATTTGTCCCATTGAGTGATGCCGTAGTCGGCGC
GATGGCCGGCTATTTATCTCTGTGGCTACTTTATTGGGTATTCAAATACGCAACGGGCAAAGAGGCGCTGGGGTACGGTG
ATTTTAAGTTGCTGGCCGCGCTGGGTGCCTGGCTGGGCTGGCAGGCGTTGCCGAATCTGGTTCTGGTGGCGGCGCTGAGT
GGGCTGGTCGTGACGCTTATTTGGCGCGGATTGCGTAAAGAGGATACGGCTAAACCGCTGGCCTTTGGCCCCTGGCTGGC
AATGGGCGGGGTATTTGGCGTGATAATGAACGGATTGAATTTGTAG

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-123)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	50.923	96.441	0.491
pilD	Vibrio campbellii strain DS40M4	45.353	95.73	0.434
pilD	Neisseria gonorrhoeae MS11	46.743	92.883	0.434
pilD	Acinetobacter baumannii D1279779	41.758	97.153	0.406
pilD	Acinetobacter nosocomialis M2	41.392	97.153	0.402