Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BWEI_RS26360 Genome accession   NZ_CP009746
Coordinates   5351723..5353099 (+) Length   458 a.a.
NCBI ID   WP_002009817.1    Uniprot ID   -
Organism   Bacillus mycoides strain WSBC10204     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5346723..5358099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWEI_RS26340 (bwei_5582) ctsR 5346922..5347383 (+) 462 WP_002029353.1 transcriptional regulator CtsR -
  BWEI_RS26345 (bwei_5583) - 5347553..5348101 (+) 549 WP_002009823.1 UvrB/UvrC motif-containing protein -
  BWEI_RS26350 (bwei_5584) - 5348106..5349170 (+) 1065 WP_002124680.1 protein arginine kinase -
  BWEI_RS26355 (bwei_5585) clpC 5349193..5351628 (+) 2436 WP_002009819.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BWEI_RS26360 (bwei_5586) radA 5351723..5353099 (+) 1377 WP_002009817.1 DNA repair protein RadA Machinery gene
  BWEI_RS26365 (bwei_5587) disA 5353103..5354176 (+) 1074 WP_000392162.1 DNA integrity scanning diadenylate cyclase DisA -
  BWEI_RS26370 (bwei_5588) - 5354337..5355440 (+) 1104 WP_002063406.1 PIN/TRAM domain-containing protein -
  BWEI_RS26375 (bwei_5589) ispD 5355457..5356137 (+) 681 WP_002009811.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BWEI_RS26380 (bwei_5590) ispF 5356251..5356727 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49828.52 Da        Isoelectric Point: 7.4250

>NTDB_id=131812 BWEI_RS26360 WP_002009817.1 5351723..5353099(+) (radA) [Bacillus mycoides strain WSBC10204]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVTQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKATAPADAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=131812 BWEI_RS26360 WP_002009817.1 5351723..5353099(+) (radA) [Bacillus mycoides strain WSBC10204]
ATGGCTAAAAAGAAAACAAAATTTACATGTCAAGAATGTGGTTATCAGTCGCCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCAGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAGGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAGTTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TCAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGATTTACAGAGAATTGCA
GCGCACATTGAAGAGATGAATCCAGCTTTTGTTGTTATTGACTCCATTCAGACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGGAGTGTAACGCAAGTACGTGAATGTACAGCGGAATTAATGAAACTTGCAAAGACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTGACAAAAGAAGGGGCAATTGCAGGACCACGTATGCTAGAACATATGGTTGATGCAGTTCTTTACTTT
GAAGGAGATCGTCACCATACATATCGTATTTTGCGAGCTGTGAAGAACCGTTTTGGTTCCACAAATGAAATGGGTATCTT
TGAAATGAAGGAACTAGGTCTTGCAGAAGTACTAAATCCTTCTGAAATTTTCCTTGAGGAACGTCCAGTTGGAGTTGCAG
GATCCACAGTGGTTGCTTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACGGGAATTGATCATAACCGCGTTTCACTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTGTTGCAAAATCAAGACGCATATTTAAAAGTTGCGGGTGGCTTGAAATTAGATGAACCAGCAATCGATCTAGCTG
TCGCCTTAAGTATTGCTTCAAGTTTCAGAGATAAAGCTACTGCACCAGCCGATGCGGTAATAGGAGAAGTAGGCCTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCGAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631

Multiple sequence alignment