Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AW20_RS22295 Genome accession   NZ_CP009541
Coordinates   3990586..3991020 (+) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus anthracis str. Sterne     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3985586..3996020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW20_RS22275 (AW20_4287) - 3986475..3987638 (-) 1164 WP_000434598.1 aminotransferase A -
  AW20_RS22280 (AW20_4288) - 3987761..3987961 (-) 201 WP_000929284.1 hypothetical protein -
  AW20_RS22285 (AW20_4289) kinB 3988272..3989546 (+) 1275 WP_000420390.1 sporulation sensor histidine kinase KinB -
  AW20_RS22290 (AW20_4290) - 3989600..3990349 (-) 750 WP_042513642.1 DUF3967 domain-containing protein -
  AW20_RS22295 (AW20_4291) nucA/comI 3990586..3991020 (+) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  AW20_RS22300 (AW20_4292) comJ 3991057..3991446 (+) 390 WP_000424033.1 competence protein ComJ -
  AW20_RS22305 (AW20_4293) metE 3991483..3993771 (-) 2289 WP_001007635.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  AW20_RS22315 (AW20_4294) - 3994389..3994946 (-) 558 WP_000062079.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=130049 AW20_RS22295 WP_000811492.1 3990586..3991020(+) (nucA/comI) [Bacillus anthracis str. Sterne]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=130049 AW20_RS22295 WP_000811492.1 3990586..3991020(+) (nucA/comI) [Bacillus anthracis str. Sterne]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAG
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATCGAATATATAAGTCCAGCAGATAACCGCGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment