Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LH20_RS03540 Genome accession   NZ_CP009452
Coordinates   765542..766903 (+) Length   453 a.a.
NCBI ID   WP_053553028.1    Uniprot ID   A0A0M3QCJ1
Organism   Sphingopyxis sp. 113P3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 760542..771903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LH20_RS03520 (LH20_03570) - 760765..762264 (+) 1500 WP_053553024.1 aldehyde dehydrogenase family protein -
  LH20_RS03525 (LH20_03575) - 762295..763788 (-) 1494 WP_083455549.1 acetyl-CoA acetyltransferase -
  LH20_RS03530 (LH20_03580) - 763796..764599 (-) 804 WP_053553026.1 enoyl-CoA hydratase-related protein -
  LH20_RS03535 (LH20_03585) fsa 764723..765373 (-) 651 WP_053553027.1 fructose-6-phosphate aldolase -
  LH20_RS03540 (LH20_03590) radA 765542..766903 (+) 1362 WP_053553028.1 DNA repair protein RadA Machinery gene
  LH20_RS03545 (LH20_03595) - 766914..767462 (+) 549 WP_200905427.1 CvpA family protein -
  LH20_RS03550 (LH20_03600) - 767459..767887 (+) 429 WP_053553029.1 iron-sulfur cluster assembly scaffold protein -
  LH20_RS03555 (LH20_03605) - 767884..769782 (+) 1899 WP_053553030.1 cation:proton antiporter -
  LH20_RS03560 (LH20_03610) aroB 769812..770909 (-) 1098 WP_053553031.1 3-dehydroquinate synthase -
  LH20_RS03565 (LH20_03615) - 770897..771460 (-) 564 WP_053553032.1 shikimate kinase -
  LH20_RS23435 - 771586..771726 (+) 141 WP_158501088.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 47592.40 Da        Isoelectric Point: 6.1814

>NTDB_id=129366 LH20_RS03540 WP_053553028.1 765542..766903(+) (radA) [Sphingopyxis sp. 113P3]
MAKAKRQYVCQNCGAVSYRWQGQCADCGEWNTLVEEAAESVFSAKHDLTKGGRALALETLNAHSPMPERMLCGIAEFDRA
LGGGFVAGSATLIGGDPGIGKSTLLLQAAGRLARAGRTVVYISGEEAAAQVRLRAQRLGLGDAPVALASATSVRDILATL
DRQTADFVVIDSIQTMHSDLIDSAPGTVSQVRASAQELIRYAKDSGAAIVLVGHVTKDGTIAGPRVLEHMVDTVLSFEGE
RSHLYRILRAVKNRFGGTDEIGVFAMGEEGLGEVANPSSLFLTDRSRDVPGSVVFPALEGTRPVLVEVQALTVRLASGAT
PRRAVVGWDSGRLAMVLAVLEARCGLQMGSAEVYLNIAGGYRLTDPAADLAVAAALISAFSDRPVPADAIVFGELSLSGE
VRPVAHDALRLREAAKLGFSRGWGPEGMKGVGGISVTGFARLGELVDLMLGRD

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=129366 LH20_RS03540 WP_053553028.1 765542..766903(+) (radA) [Sphingopyxis sp. 113P3]
ATGGCCAAAGCGAAACGCCAATATGTCTGTCAGAATTGCGGTGCGGTGTCGTACCGCTGGCAGGGGCAATGCGCGGATTG
CGGGGAATGGAACACGCTTGTCGAGGAGGCGGCCGAATCCGTCTTTTCAGCAAAGCATGACCTCACCAAAGGCGGGCGCG
CGCTAGCGCTCGAAACGTTGAATGCGCACAGCCCCATGCCCGAACGGATGCTCTGCGGAATTGCCGAGTTCGACCGTGCG
CTAGGGGGCGGTTTCGTTGCCGGGTCGGCGACCTTGATCGGGGGCGATCCCGGGATCGGCAAGTCGACGCTGCTGCTCCA
GGCAGCGGGCAGGCTCGCGCGGGCCGGCCGAACGGTCGTCTATATCAGCGGCGAGGAGGCTGCAGCGCAGGTCCGGCTTC
GCGCGCAGCGGCTGGGGCTCGGCGATGCCCCGGTCGCGCTTGCAAGCGCGACCTCGGTGCGCGACATTCTCGCAACGCTC
GACCGGCAGACGGCCGATTTCGTCGTCATCGATTCGATCCAGACGATGCACAGCGACCTCATCGACAGCGCGCCGGGGAC
GGTGAGCCAGGTACGCGCAAGCGCGCAGGAGCTGATCCGTTACGCCAAAGACAGCGGGGCAGCGATCGTGCTCGTCGGGC
ACGTGACGAAGGATGGCACGATCGCCGGGCCGCGCGTGCTTGAACATATGGTCGACACGGTGCTGTCCTTCGAAGGGGAG
CGCAGCCACCTTTATCGCATCCTTCGTGCGGTGAAGAACCGCTTCGGCGGTACGGACGAGATCGGTGTATTCGCAATGGG
CGAAGAGGGCCTCGGCGAAGTCGCCAACCCCTCGAGCCTTTTCCTCACCGATCGCAGCCGCGATGTCCCGGGGTCGGTGG
TCTTCCCCGCGCTCGAGGGGACACGGCCGGTCCTCGTTGAGGTGCAGGCGCTCACCGTGCGGCTCGCGAGCGGTGCAACG
CCCCGCCGCGCGGTCGTCGGATGGGACAGCGGGCGGCTTGCGATGGTGCTCGCGGTGCTCGAGGCGCGCTGCGGGCTTCA
GATGGGGAGCGCGGAAGTCTATCTCAACATTGCGGGCGGCTACCGCCTGACCGATCCGGCGGCGGACCTTGCTGTCGCGG
CCGCGCTGATCTCGGCCTTCAGCGACCGGCCGGTGCCTGCCGATGCGATCGTCTTCGGCGAGCTATCGCTCTCGGGCGAG
GTGCGCCCCGTTGCGCACGACGCCCTCCGGCTGCGTGAGGCGGCAAAGCTCGGTTTTTCGCGCGGCTGGGGGCCTGAGGG
GATGAAAGGCGTCGGCGGGATTTCGGTGACGGGTTTTGCGCGTCTCGGCGAACTCGTTGACCTGATGCTCGGGCGCGACT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(342-419)

AAA domain (AAA_25)

(81-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M3QCJ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

47.516

100

0.486
  radA Streptococcus mitis NCTC 12261

47.007

99.558

0.468
  radA Streptococcus pneumoniae Rx1

48.598

94.481

0.459
  radA Streptococcus pneumoniae R6

48.598

94.481

0.459
  radA Streptococcus pneumoniae TIGR4

48.598

94.481

0.459
  radA Streptococcus pneumoniae D39

48.598

94.481

0.459
  radA Streptococcus mitis SK321

48.598

94.481

0.459

Multiple sequence alignment