Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   NJ8700_RS00030 Genome accession   NZ_CP009230
Coordinates   7273..7827 (+) Length   184 a.a.
NCBI ID   WP_005701647.1    Uniprot ID   -
Organism   Aggregatibacter aphrophilus NJ8700     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2273..12827
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NJ8700_RS00015 (NJ8700_00015) - 3254..4585 (-) 1332 WP_005701652.1 DUF4123 domain-containing protein -
  NJ8700_RS00020 (NJ8700_00020) ansB 4749..5798 (-) 1050 WP_005701650.1 L-asparaginase 2 -
  NJ8700_RS00025 (NJ8700_00025) - 6097..7251 (+) 1155 WP_005701648.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  NJ8700_RS00030 (NJ8700_00030) pilF/pilF2 7273..7827 (+) 555 WP_005701647.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  NJ8700_RS00035 (NJ8700_00035) - 8046..9071 (+) 1026 WP_005701646.1 RodZ family helix-turn-helix domain-containing protein -
  NJ8700_RS00040 (NJ8700_00040) ispG 9082..10185 (+) 1104 WP_005701645.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  NJ8700_RS00045 (NJ8700_00045) hisS 10210..11487 (+) 1278 WP_005701644.1 histidine--tRNA ligase -
  NJ8700_RS00050 (NJ8700_00050) - 11498..12112 (+) 615 WP_005701643.1 YfgM family protein -
  NJ8700_RS00055 (NJ8700_00055) - 12168..12620 (+) 453 WP_005701641.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20928.78 Da        Isoelectric Point: 7.3439

>NTDB_id=127395 NJ8700_RS00030 WP_005701647.1 7273..7827(+) (pilF/pilF2) [Aggregatibacter aphrophilus NJ8700]
MANIQFLFYRFVPFIFPFLFSACVVHSDAPDFDKQAAAKARVELALGHLQQQDGSQAKLNLDKALSYAPKYYLVHAALAY
FYQQQGDVEHAKQSYLTAIKLDDKQGDVFNNFGAFLCSQGEYQAAYEQFKQALKSPNYYHQADTYENLALCALSAKEQKI
YQENLAALEKIEPARAKKLTQFVK

Nucleotide


Download         Length: 555 bp        

>NTDB_id=127395 NJ8700_RS00030 WP_005701647.1 7273..7827(+) (pilF/pilF2) [Aggregatibacter aphrophilus NJ8700]
ATGGCCAATATTCAGTTTCTTTTTTACCGTTTTGTACCATTTATTTTTCCTTTTCTGTTTTCTGCCTGTGTTGTCCATTC
CGATGCACCGGATTTTGATAAACAAGCTGCGGCAAAAGCCCGTGTAGAGTTGGCATTGGGCCATTTACAGCAGCAAGATG
GCAGCCAGGCCAAGTTGAATTTGGATAAAGCCCTTTCTTATGCCCCAAAATATTATTTGGTACATGCCGCGTTAGCCTAT
TTTTATCAGCAACAAGGGGATGTTGAACACGCCAAACAATCGTATTTAACGGCGATTAAATTGGATGATAAACAAGGGGA
TGTATTCAATAATTTCGGTGCGTTTCTTTGCTCACAAGGAGAATATCAGGCCGCGTATGAACAATTTAAACAGGCATTAA
AAAGCCCGAATTATTATCATCAAGCTGACACCTACGAAAACCTTGCACTGTGTGCGTTATCGGCAAAAGAGCAGAAAATT
TATCAAGAGAACCTTGCTGCTCTTGAAAAGATTGAACCGGCACGAGCCAAGAAATTAACTCAGTTCGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(75-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.831

90.217

0.522
  pilF Glaesserella parasuis strain SC1401

42.162

100

0.424

Multiple sequence alignment