Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ID12_RS15950 Genome accession   NZ_CP009108
Coordinates   3164936..3166006 (-) Length   356 a.a.
NCBI ID   WP_035390817.1    Uniprot ID   -
Organism   Bacillus altitudinis strain GR-8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3159936..3171006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ID12_RS15900 (ID12_16545) sipW 3160026..3160598 (-) 573 WP_017357979.1 signal peptidase I SipW -
  ID12_RS15905 (ID12_16550) tapA 3160648..3161175 (-) 528 WP_050827403.1 amyloid fiber anchoring/assembly protein TapA -
  ID12_RS15910 (ID12_16555) - 3161428..3161754 (+) 327 WP_048001387.1 DUF3889 domain-containing protein -
  ID12_RS15915 (ID12_16560) - 3161788..3161988 (-) 201 WP_046343970.1 YqzE family protein -
  ID12_RS15920 (ID12_16565) - 3162049..3162432 (-) 384 WP_050827405.1 competence type IV pilus minor pilin ComGG -
  ID12_RS15925 (ID12_16570) comGF 3162429..3162926 (-) 498 WP_046343974.1 competence type IV pilus minor pilin ComGF -
  ID12_RS15930 (ID12_16575) comGE 3162868..3163182 (-) 315 WP_007501227.1 competence type IV pilus minor pilin ComGE -
  ID12_RS15935 (ID12_16580) comGD 3163166..3163612 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  ID12_RS15940 (ID12_16585) comGC 3163605..3163898 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  ID12_RS15945 (ID12_16590) comGB 3163915..3164955 (-) 1041 WP_035390815.1 competence type IV pilus assembly protein ComGB -
  ID12_RS15950 (ID12_16595) comGA 3164936..3166006 (-) 1071 WP_035390817.1 competence type IV pilus ATPase ComGA Machinery gene
  ID12_RS15955 (ID12_16600) - 3166464..3167336 (-) 873 WP_035390819.1 STAS domain-containing protein -
  ID12_RS15965 (ID12_16615) - 3167562..3167942 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ID12_RS15970 (ID12_16620) - 3168155..3168397 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  ID12_RS15975 (ID12_16625) - 3168446..3169084 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  ID12_RS19145 (ID12_16630) - 3169245..3169418 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  ID12_RS15980 (ID12_16635) - 3169443..3169757 (-) 315 WP_008342314.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40396.75 Da        Isoelectric Point: 9.0449

>NTDB_id=126882 ID12_RS15950 WP_035390817.1 3164936..3166006(-) (comGA) [Bacillus altitudinis strain GR-8]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASTRLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEANL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=126882 ID12_RS15950 WP_035390817.1 3164936..3166006(-) (comGA) [Bacillus altitudinis strain GR-8]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGTTAGAAGAGGCGTGCCGAATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGCTAAGACGTGGTCAAGTGTTTGTTCGGCTCTCTACACTACCAACTGTAAATGATGAAAGTCTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCTAAAACAAAGTATTTGTCACTGTTCCCGAAAGCTTCAACGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACAGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATTCAGTTT
GCCAAAAAACATTTTAATCGGAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGTTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCCAGAACAGCTATTAGAGCAGCTTTGACAGGCCATTTAGTCATGAGCACACTGCAT
GCAAAAAATGCAAAAGGAGCTCTTTATCGAATGCTGGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAACCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.607

100

0.646

Multiple sequence alignment