Detailed information NTG0125580

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: FORC12_RS10270
• Genome accession: NZ_CP010998
• Coordinates: 1993102..1994214 (+)
• Length: 370 a.a.
• NCBI ID: WP_000775113.1
• Uniprot ID: P68863
• Organism: Staphylococcus aureus strain FORC_012 isolate human sputum
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1988102..1999214

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC12_RS10245 (FORC12_1851)	rnpA	1988359..1988712 (-)	354	WP_001789343.1	ribonuclease P protein component	-
FORC12_RS10250	rpmH	1988833..1988970 (-)	138	WP_000240855.1	50S ribosomal protein L34	-
FORC12_RS10255 (FORC12_1852)	dnaA	1989701..1991062 (+)	1362	WP_001290433.1	chromosomal replication initiator protein DnaA	-
FORC12_RS10260 (FORC12_1853)	dnaN	1991340..1992473 (+)	1134	WP_000969811.1	DNA polymerase III subunit beta	-
FORC12_RS10265 (FORC12_1854)	yaaA	1992860..1993105 (+)	246	WP_001789359.1	S4 domain-containing protein YaaA	-
FORC12_RS10270 (FORC12_1855)	recF	1993102..1994214 (+)	1113	WP_000775113.1	DNA replication/repair protein RecF	Machinery gene
FORC12_RS10275 (FORC12_1856)	gyrB	1994224..1996158 (+)	1935	WP_000255586.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
FORC12_RS10280 (FORC12_1857)	gyrA	1996195..1998864 (+)	2670	WP_000819083.1	DNA gyrase subunit A	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 42415.43 Da
• Isoelectric Point: 6.4362

>NTDB_id=125580 FORC12_RS10270 WP_000775113.1 1993102..1994214(+) (recF) [Staphylococcus aureus strain FORC_012 isolate human sputum]
MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIYTLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGT
MPLTMFITKKGKQVKVNHLEQSRLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDKEALSLNYLPSLKFDYAQNEAAR
LEEIMSILSDNMQREKERGISLFGPHRDDISFDVNGMDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLS
ELDDSRQTHLLSTIQHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK

Nucleotide

• Length: 1113 bp

>NTDB_id=125580 FORC12_RS10270 WP_000775113.1 1993102..1994214(+) (recF) [Staphylococcus aureus strain FORC_012 isolate human sputum]
ATGAAGCTAAATACACTCCAATTAGAAAATTATCGTAACTATGATGAGGTTACGTTGAAATGTCATCCTGACGTGAATAT
CCTCATTGGAGAAAATGCACAAGGAAAGACAAATTTACTTGAATCAATTTATACCTTAGCTTTAGCAAAAAGTCATAGAA
CGAGTAATGATAAGGAACTCATACGTTTTAATGCTGATTATGCTAAAATAGAAGGTGAGCTTAGTTATAGACACGGCACG
ATGCCATTAACAATGTTTATAACTAAAAAAGGTAAACAAGTCAAAGTGAATCACTTAGAGCAAAGTCGTCTAACTCAATA
TATTGGACACCTCAATGTGGTTCTATTTGCGCCAGAAGATTTGAATATTGTAAAAGGCTCTCCTCAAATAAGACGACGCT
TTATAGATATGGAGTTGGGCCAAATTTCTGCTGTTTACTTAAATGATTTAGCTCAATACCAACGTATTTTAAAGCAAAAG
AATAATTACTTAAAGCAGTTACAATTAGGCCAAAAAAAGGACTTAACAATGTTGGAAGTATTAAATCAGCAGTTTGCTGA
ATATGCAATGAAAGTAACTGATAAACGTGCACATTTTATTCAAGAGCTAGAGTCGTTAGCTAAACCGATTCATGCTGGTA
TCACAAATGATAAAGAAGCGTTGTCGCTGAATTATTTACCTAGTCTTAAATTTGATTATGCTCAAAATGAAGCGGCACGA
CTTGAAGAAATTATGTCTATTCTTAGCGATAATATGCAAAGAGAAAAAGAACGAGGCATTAGCTTATTCGGACCACATCG
AGATGATATAAGTTTTGATGTGAATGGCATGGATGCTCAAACATATGGTTCTCAAGGACAGCAACGTACAACGGCTTTGT
CCATTAAATTAGCTGAAATTGAGTTAATGAATATCGAAGTTGGGGAATATCCCATCTTATTATTAGACGATGTACTCAGT
GAATTAGATGATTCGCGTCAAACGCATTTATTAAGTACGATTCAGCATAAAGTACAAACATTTGTCACTACGACATCTGT
AGATGGTATTGATCATGAAATCATGAATAACGCTAAATTGTATCGTATTAATCAAGGTGAAATTATAAAGTAA

Domains

Predicted by InterProScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (3-347)

3D structure

• AlphaFold DB: P68863

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Bacillus subtilis subsp. subtilis str. 168	58.649	100	0.586
recF	Streptococcus pneumoniae R6	49.33	100	0.497