Detailed information NTG0125105

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HW01_RS25810
• Genome accession: NZ_CP008866
• Coordinates: 5441934..5442806 (+)
• Length: 290 a.a.
• NCBI ID: WP_016852118.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain T38079
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5436934..5447806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW01_RS25795 (HW01_22245)	-	5438317..5438781 (-)	465	WP_003122079.1	pilin	-
HW01_RS25800 (HW01_22240)	pilB	5439012..5440712 (+)	1701	WP_003122078.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HW01_RS25805 (HW01_22235)	pilC	5440716..5441933 (+)	1218	WP_003107299.1	type II secretion system F family protein	Machinery gene
HW01_RS25810 (HW01_22230)	pilD	5441934..5442806 (+)	873	WP_016852118.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HW01_RS25815 (HW01_22225)	coaE	5442803..5443414 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
HW01_RS25820 (HW01_22220)	yacG	5443411..5443611 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HW01_RS25825 (HW01_22215)	-	5443648..5443857 (-)	210	WP_003094660.1	hypothetical protein	-
HW01_RS25830 (HW01_22210)	-	5443963..5444652 (-)	690	WP_003103868.1	energy-coupling factor ABC transporter permease	-
HW01_RS25835 (HW01_22205)	-	5444649..5445119 (-)	471	WP_003094664.1	hypothetical protein	-
HW01_RS25840 (HW01_22200)	-	5445116..5445541 (-)	426	WP_003116252.1	GNAT family N-acetyltransferase	-
HW01_RS25845 (HW01_22195)	-	5445674..5446303 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
HW01_RS25850 (HW01_22190)	-	5446300..5446749 (+)	450	WP_003094670.1	MOSC domain-containing protein	-
HW01_RS25855 (HW01_32815)	-	5446775..5446945 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31845.85 Da
• Isoelectric Point: 7.7068

>NTDB_id=125105 HW01_RS25810 WP_016852118.1 5441934..5442806(+) (pilD) [Pseudomonas aeruginosa strain T38079]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLASYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=125105 HW01_RS25810 WP_016852118.1 5441934..5442806(+) (pilD) [Pseudomonas aeruginosa strain T38079]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGCCAGCTACCTGGCGCTGGGCGGCAAGTGTTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATACTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.357	96.552	0.534
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	48.561	95.862	0.466