Detailed information NTG0125027

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HV95_RS05800
• Genome accession: NZ_CP008864
• Coordinates: 1219316..1220188 (-)
• Length: 290 a.a.
• NCBI ID: WP_023084740.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain W60856
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1214316..1225188

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HV95_RS05755 (HV95_32415)	-	1215177..1215347 (-)	171	WP_003094672.1	DUF3094 family protein	-
HV95_RS05760 (HV95_31490)	-	1215373..1215822 (-)	450	WP_003094670.1	MOSC domain-containing protein	-
HV95_RS05765 (HV95_31495)	-	1215819..1216448 (-)	630	WP_003094668.1	DUF1780 domain-containing protein	-
HV95_RS05770 (HV95_31500)	-	1216581..1217006 (+)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
HV95_RS05775 (HV95_31505)	-	1217003..1217473 (+)	471	WP_003094664.1	hypothetical protein	-
HV95_RS05780 (HV95_31510)	-	1217470..1218159 (+)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
HV95_RS05785 (HV95_31515)	-	1218265..1218474 (+)	210	WP_003094660.1	hypothetical protein	-
HV95_RS05790 (HV95_31520)	yacG	1218511..1218711 (-)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
HV95_RS05795 (HV95_31525)	coaE	1218708..1219319 (-)	612	WP_003112838.1	dephospho-CoA kinase	-
HV95_RS05800 (HV95_31530)	pilD	1219316..1220188 (-)	873	WP_023084740.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
HV95_RS05805 (HV95_31535)	pilC	1220189..1221406 (-)	1218	WP_003161763.1	type II secretion system F family protein	Machinery gene
HV95_RS05810 (HV95_31540)	pilB	1221410..1223110 (-)	1701	WP_003107297.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HV95_RS05815 (HV95_31545)	-	1223341..1223805 (+)	465	WP_023086642.1	pilin	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31843.88 Da
• Isoelectric Point: 7.7068

>NTDB_id=125027 HV95_RS05800 WP_023084740.1 1219316..1220188(-) (pilD) [Pseudomonas aeruginosa strain W60856]
MTLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=125027 HV95_RS05800 WP_023084740.1 1219316..1220188(-) (pilD) [Pseudomonas aeruginosa strain W60856]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGTGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.714	96.552	0.538
pilD	Vibrio campbellii strain DS40M4	53.004	97.586	0.517
pilD	Acinetobacter nosocomialis M2	50.53	97.586	0.493
pilD	Acinetobacter baumannii D1279779	50.53	97.586	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472