Detailed information NTG0124836

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HW09_RS23500
• Genome accession: NZ_CP008857
• Coordinates: 5116047..5117195 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F30658
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5111047..5122195

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HW09_RS23475	-	5112198..5112494 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
HW09_RS23480 (HW09_23735)	-	5112491..5113084 (-)	594	WP_003084544.1	YggT family protein	-
HW09_RS23485 (HW09_23740)	proC	5113095..5113916 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
HW09_RS23490 (HW09_23745)	-	5113928..5114620 (-)	693	WP_015649764.1	YggS family pyridoxal phosphate-dependent enzyme	-
HW09_RS23495 (HW09_23750)	pilT	5114835..5115869 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
HW09_RS23500 (HW09_23755)	pilU	5116047..5117195 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
HW09_RS23505 (HW09_23760)	czcD	5117203..5118102 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
HW09_RS23510 (HW09_23765)	-	5118256..5118660 (+)	405	WP_003084560.1	NINE protein	-
HW09_RS23515 (HW09_23770)	-	5118903..5120276 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
HW09_RS23520 (HW09_23775)	-	5120273..5121457 (+)	1185	WP_015649763.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=124836 HW09_RS23500 WP_003100959.1 5116047..5117195(+) (pilU) [Pseudomonas aeruginosa strain F30658]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=124836 HW09_RS23500 WP_003100959.1 5116047..5117195(+) (pilU) [Pseudomonas aeruginosa strain F30658]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382