Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DR63_RS12730 Genome accession   NZ_CP008785
Coordinates   2577145..2578653 (-) Length   502 a.a.
NCBI ID   WP_009893048.1    Uniprot ID   -
Organism   Burkholderia thailandensis E264     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2572145..2583653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DR63_RS12700 (DR63_2260) - 2573168..2573800 (-) 633 WP_009893059.1 LON peptidase substrate-binding domain-containing protein -
  DR63_RS12705 (DR63_2261) rapZ 2573827..2574720 (-) 894 WP_009893058.1 RNase adapter RapZ -
  DR63_RS12710 (DR63_2262) hprK 2574797..2575765 (-) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  DR63_RS12715 (DR63_2263) - 2575894..2576403 (-) 510 WP_161654448.1 PTS sugar transporter subunit IIA -
  DR63_RS12720 (DR63_2264) hpf 2576636..2576995 (-) 360 WP_009893049.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DR63_RS12730 (DR63_2265) rpoN/rpoN1 2577145..2578653 (-) 1509 WP_009893048.1 RNA polymerase factor sigma-54 Machinery gene
  DR63_RS12735 (DR63_2266) lptB 2578824..2579600 (-) 777 WP_009893047.1 LPS export ABC transporter ATP-binding protein -
  DR63_RS12740 (DR63_2267) lptA 2579597..2580262 (-) 666 WP_009893045.1 lipopolysaccharide transport periplasmic protein LptA -
  DR63_RS12745 (DR63_2268) lptC 2580281..2580895 (-) 615 WP_009893044.1 LPS export ABC transporter periplasmic protein LptC -
  DR63_RS12750 (DR63_2269) - 2580900..2581439 (-) 540 WP_009893042.1 KdsC family phosphatase -
  DR63_RS12755 (DR63_2270) kdsD 2581439..2582422 (-) 984 WP_009893041.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54981.05 Da        Isoelectric Point: 4.8782

>NTDB_id=124234 DR63_RS12730 WP_009893048.1 2577145..2578653(-) (rpoN/rpoN1) [Burkholderia thailandensis E264]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSSPEPMS
GASNGTTATNERTERDETAGVDEYDGYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLDEVLADLPAELEVDVDELNAALALVHSFDPAGVGARSASECLKLQLLRLDPSPTRT
LALDIVSQHLELLAARDFTRLRKQLKATDDELRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQSWQAELNPEVV
PKLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=124234 DR63_RS12730 WP_009893048.1 2577145..2578653(-) (rpoN/rpoN1) [Burkholderia thailandensis E264]
ATGAAAGCCAGTCTCCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAATCGATCCGGCTGCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCAACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGACGACTGGA
TCGCGAGCCCCCTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCTCGCCCGAGCCGATGTCC
GGCGCGTCGAACGGCACGACTGCGACGAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACGGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAATCTCGACGACTTCGGCCGCTCCCCCGCCGCATCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAAACCTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGACGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAAGTCGACGTCGACGAACTGAACGCGGCGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGGTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGGCTGGATCCGTCCCCGACGCGCACG
CTCGCGCTCGATATCGTGTCGCAGCATCTCGAACTGCTTGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGAGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTTCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAGCTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGTATCAACCATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
GCAGCTTCAGGAAGCGCGCTGGCTGATCAAGAATATTCAGCAGCGGTTCGAGACGATCCTGCGGGTCGCGCAGGCGATTG
TCGAACGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACTCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGGTCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.504

100

0.693

Multiple sequence alignment