Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   C645_RS02330 Genome accession   NZ_CP008740
Coordinates   467743..468282 (+) Length   179 a.a.
NCBI ID   WP_005659915.1    Uniprot ID   -
Organism   Haemophilus influenzae 2019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 462743..473282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C645_RS02310 (C645_02335) - 463200..464120 (-) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -
  C645_RS02315 (C645_02340) - 464120..465001 (-) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  C645_RS02320 (C645_02345) - 465366..466244 (-) 879 WP_005659920.1 D-alanyl-D-alanine carboxypeptidase family protein -
  C645_RS02325 (C645_02350) - 466491..467642 (+) 1152 WP_005659917.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  C645_RS02330 (C645_02355) pilF/pilF2 467743..468282 (+) 540 WP_005659915.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  C645_RS02335 (C645_02360) - 468354..469265 (+) 912 WP_005688933.1 helix-turn-helix domain-containing protein -
  C645_RS02340 (C645_02365) ispG 469274..470380 (+) 1107 WP_005656366.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  C645_RS02345 (C645_02370) hisS 470390..471661 (+) 1272 WP_005656367.1 histidine--tRNA ligase -
  C645_RS02350 (C645_02375) - 471679..472293 (+) 615 WP_005656369.1 YfgM family protein -
  C645_RS02355 (C645_02380) iscX 472344..472538 (-) 195 WP_005656371.1 Fe-S cluster assembly protein IscX -
  C645_RS02360 (C645_02385) fdx 472538..472879 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20602.19 Da        Isoelectric Point: 8.2626

>NTDB_id=123952 C645_RS02330 WP_005659915.1 467743..468282(+) (pilF/pilF2) [Haemophilus influenzae 2019]
MKTISKQLSAVIFPFIFSACVSQSASNLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSSQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=123952 C645_RS02330 WP_005659915.1 467743..468282(+) (pilF/pilF2) [Haemophilus influenzae 2019]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCTCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.207

100

0.972
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment