Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   H733_RS01345 Genome accession   NZ_CP007805
Coordinates   274981..275520 (+) Length   179 a.a.
NCBI ID   WP_038439288.1    Uniprot ID   -
Organism   Haemophilus influenzae CGSHiCZ412602     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269981..280520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H733_RS01325 (H733_0254) - 270438..271358 (-) 921 WP_005669098.1 ATP-binding cassette domain-containing protein -
  H733_RS01330 (H733_0255) - 271358..272239 (-) 882 WP_038439284.1 metal ABC transporter substrate-binding protein -
  H733_RS09240 (H733_0256) - 272487..272612 (+) 126 WP_257005528.1 hypothetical protein -
  H733_RS01335 (H733_0257) - 272604..273482 (-) 879 WP_038439286.1 D-alanyl-D-alanine carboxypeptidase family protein -
  H733_RS01340 (H733_0259) - 273729..274880 (+) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  H733_RS01345 (H733_0260) pilF/pilF2 274981..275520 (+) 540 WP_038439288.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  H733_RS01350 (H733_0261) - 275592..276503 (+) 912 WP_005666610.1 helix-turn-helix domain-containing protein -
  H733_RS01355 (H733_0262) ispG 276512..277618 (+) 1107 WP_038439289.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  H733_RS01360 (H733_0263) hisS 277628..278899 (+) 1272 WP_038439291.1 histidine--tRNA ligase -
  H733_RS01365 (H733_0264) - 278917..279531 (+) 615 WP_038439292.1 YfgM family protein -
  H733_RS01370 (H733_0265) iscX 279582..279776 (-) 195 WP_014550441.1 Fe-S cluster assembly protein IscX -
  H733_RS01375 (H733_0266) fdx 279776..280117 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20583.32 Da        Isoelectric Point: 9.4145

>NTDB_id=123478 H733_RS01345 WP_038439288.1 274981..275520(+) (pilF/pilF2) [Haemophilus influenzae CGSHiCZ412602]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIKNAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQTDTFENIALCAYSAKKMNIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=123478 H733_RS01345 WP_038439288.1 274981..275520(+) (pilF/pilF2) [Haemophilus influenzae CGSHiCZ412602]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGCGTCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAAAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAACAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

94.972

100

0.95
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment