Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BMMGA3_RS11175 Genome accession   NZ_CP007739
Coordinates   2301485..2302567 (-) Length   360 a.a.
NCBI ID   WP_004435641.1    Uniprot ID   I3EAF8
Organism   Bacillus methanolicus MGA3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2296485..2307567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMMGA3_RS11130 (BMMGA3_11565) - 2297021..2297812 (+) 792 WP_004435620.1 YqhG family protein -
  BMMGA3_RS11135 (BMMGA3_11570) - 2297858..2298034 (-) 177 WP_081485685.1 YqzE family protein -
  BMMGA3_RS11140 (BMMGA3_11575) - 2298077..2298391 (-) 315 WP_412150331.1 shikimate kinase -
  BMMGA3_RS18655 (BMMGA3_11580) - 2298447..2298581 (-) 135 WP_318533204.1 shikimate kinase -
  BMMGA3_RS11145 (BMMGA3_11585) comGG 2298595..2298981 (-) 387 WP_004435627.1 competence type IV pilus minor pilin ComGG -
  BMMGA3_RS11150 (BMMGA3_11590) comGF 2298978..2299451 (-) 474 WP_237712842.1 competence type IV pilus minor pilin ComGF -
  BMMGA3_RS11155 (BMMGA3_11595) - 2299402..2299731 (-) 330 WP_004435631.1 hypothetical protein -
  BMMGA3_RS11160 (BMMGA3_11600) comGD 2299706..2300155 (-) 450 WP_004435633.1 competence type IV pilus minor pilin ComGD -
  BMMGA3_RS11165 (BMMGA3_11605) comGC 2300152..2300463 (-) 312 WP_004435635.1 competence type IV pilus major pilin ComGC Machinery gene
  BMMGA3_RS11170 (BMMGA3_11610) comGB 2300479..2301510 (-) 1032 WP_004435638.1 competence type IV pilus assembly protein ComGB -
  BMMGA3_RS11175 (BMMGA3_11615) comGA 2301485..2302567 (-) 1083 WP_004435641.1 competence type IV pilus ATPase ComGA Machinery gene
  BMMGA3_RS11185 (BMMGA3_11625) - 2302927..2303628 (+) 702 WP_004435643.1 helix-turn-helix transcriptional regulator -
  BMMGA3_RS11190 (BMMGA3_11630) - 2303712..2303957 (+) 246 WP_004435645.1 DUF2626 domain-containing protein -
  BMMGA3_RS11195 (BMMGA3_11635) - 2303995..2305104 (-) 1110 WP_004435648.1 class I SAM-dependent methyltransferase -
  BMMGA3_RS11200 (BMMGA3_11640) - 2305293..2305928 (-) 636 WP_004435650.1 MBL fold metallo-hydrolase -
  BMMGA3_RS11205 (BMMGA3_11645) - 2306007..2306501 (+) 495 WP_004435653.1 hypothetical protein -
  BMMGA3_RS17465 (BMMGA3_11650) - 2306598..2306771 (+) 174 WP_004435655.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40667.98 Da        Isoelectric Point: 8.0584

>NTDB_id=123005 BMMGA3_RS11175 WP_004435641.1 2301485..2302567(-) (comGA) [Bacillus methanolicus MGA3]
MSSIKQLADRIIRDAVRNQATDIHIIPRKKDTLIQLRMGNRLVPRLYLPKEECDRLISHFKFTALMDIGERRRPQSGAFS
FIVDGDAIGLRLSTLPANSSESLVIRILPQQKQIPFYQLSLFPSITRKLLALLKHAHGLIIFTGPTGSGKTTTLYSLLNE
TSHLFNRNVITLEDPIEKDADSVLQVQVNEKAGVSYAAGLKAILRHDPDIIMVGEIRDAETAKIAVRAALTGHLVLSTMH
TRDAKGAIYRLIEFGVNWLEVEQTLVAVTAQRLVELTCPFCEEECSPYCFSYGRWKRASVFEILHGRALSSVMRRANGED
FEASYRTLKEMIKKGIALGYIKESEYDRWVYDDESLEMEG

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=123005 BMMGA3_RS11175 WP_004435641.1 2301485..2302567(-) (comGA) [Bacillus methanolicus MGA3]
GTGAGTTCAATAAAACAGTTGGCTGACAGAATTATAAGAGATGCTGTCCGAAATCAAGCTACAGACATTCACATTATCCC
GAGAAAAAAAGATACCCTGATACAGCTTCGAATGGGCAACCGTCTAGTCCCTCGACTTTACCTCCCAAAAGAAGAATGTG
ACCGACTCATCTCTCATTTTAAATTTACGGCTTTAATGGATATCGGAGAAAGAAGGCGTCCCCAGAGCGGAGCTTTTTCC
TTCATAGTCGATGGCGATGCAATCGGACTTCGTTTATCCACCCTTCCTGCCAACAGTAGTGAAAGTCTTGTTATCCGAAT
ACTTCCTCAGCAAAAGCAAATTCCTTTTTATCAATTATCCTTATTTCCAAGCATTACACGAAAACTATTAGCGCTACTTA
AACACGCTCATGGCCTAATTATTTTCACCGGACCAACCGGAAGCGGCAAGACCACTACCCTTTATTCACTCCTAAATGAA
ACTTCCCATTTATTTAATCGAAATGTGATTACGTTAGAGGATCCGATCGAAAAAGATGCTGATTCTGTTCTGCAAGTGCA
AGTAAATGAAAAAGCGGGAGTATCGTATGCTGCTGGCTTAAAAGCTATTTTGCGGCACGACCCTGACATTATCATGGTCG
GAGAGATAAGAGATGCGGAAACAGCAAAAATTGCCGTTCGGGCTGCTTTAACGGGACATCTTGTTTTGAGCACAATGCAT
ACAAGGGATGCGAAGGGAGCGATCTACAGGCTGATAGAATTTGGTGTGAATTGGCTAGAAGTAGAACAAACGCTTGTAGC
GGTAACTGCCCAGCGTCTTGTCGAATTAACTTGTCCATTTTGCGAAGAAGAGTGCTCTCCTTATTGCTTTTCGTACGGAA
GGTGGAAAAGAGCAAGTGTTTTCGAGATTTTGCACGGCCGGGCTCTATCCTCTGTCATGAGAAGAGCTAACGGAGAAGAT
TTTGAGGCTTCGTACAGGACGTTAAAGGAAATGATCAAAAAAGGAATTGCACTTGGCTATATTAAGGAATCGGAATATGA
CAGGTGGGTGTATGATGATGAATCGCTCGAAATGGAAGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3EAF8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.62

98.611

0.558
  pilB Glaesserella parasuis strain SC1401

40

97.222

0.389

Multiple sequence alignment