Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BMMGA3_RS04175 Genome accession   NZ_CP007739
Coordinates   834940..838722 (+) Length   1260 a.a.
NCBI ID   WP_034669194.1    Uniprot ID   -
Organism   Bacillus methanolicus MGA3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 829940..843722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMMGA3_RS04160 (BMMGA3_04240) - 829972..830583 (+) 612 WP_004433472.1 TVP38/TMEM64 family protein -
  BMMGA3_RS04165 (BMMGA3_04245) lepB 830621..831175 (+) 555 WP_004433475.1 signal peptidase I -
  BMMGA3_RS04170 (BMMGA3_04250) addB 831459..834980 (+) 3522 WP_004433478.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BMMGA3_RS04175 (BMMGA3_04255) addA 834940..838722 (+) 3783 WP_034669194.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BMMGA3_RS04180 (BMMGA3_04260) - 838765..838986 (-) 222 WP_004433484.1 spore germination protein -
  BMMGA3_RS04185 (BMMGA3_04265) - 839052..839441 (-) 390 WP_004433488.1 spore germination protein GerPE -
  BMMGA3_RS04190 (BMMGA3_04270) - 839443..839625 (-) 183 WP_004433491.1 hypothetical protein -
  BMMGA3_RS04195 (BMMGA3_04275) gerPC 839622..840230 (-) 609 WP_004433494.1 spore germination protein GerPC -
  BMMGA3_RS04200 (BMMGA3_04280) - 840253..840471 (-) 219 WP_004433496.1 spore germination protein GerPB -
  BMMGA3_RS04205 (BMMGA3_04285) - 840489..840710 (-) 222 WP_004433500.1 spore germination protein -
  BMMGA3_RS04210 (BMMGA3_04290) - 841163..842353 (+) 1191 WP_004433504.1 DUF418 domain-containing protein -
  BMMGA3_RS04215 (BMMGA3_04295) - 842395..843306 (+) 912 WP_004433508.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 1260 a.a.        Molecular weight: 145221.61 Da        Isoelectric Point: 4.9186

>NTDB_id=122985 BMMGA3_RS04175 WP_034669194.1 834940..838722(+) (addA) [Bacillus methanolicus MGA3]
MILTNSVIIPPKPENVTWTDDQWKAIMAKDRDILVAAAAGSGKTAVLVERIIRRITSLHDPIDVDQLLVVTFTNASAAEM
RHRIGEALEKEINQNPTSAHLRKQLSLLNKASISTLHSFCLEVIKKYYYLIDIDPGFRIADETEAQLMRDEVLDELFEEE
YGKSDNEAFFSLVDTFTNDRSDEALQDIIRELYDFARSNPSPDRYLKSIIEMYDVDENGKLEDLPFMDALLDDIEMQLEG
AKQMLLEGLELTKLPGGPAPRAENFIDDLHIVDTLISAKNDSWSTLYNAMQNLSFSRAKTVRGDEYDGDLVEKAQKLRER
AKKVLQDLQSELFSRRPESFLKDMREMKGHIETLISLVKSFSDRFEQVKREKGLVDFADLEHYCLDILTGSIDEDGRRIP
SEAALSYRRLFKEVLVDEYQDTNMVQEAILQLVTNEEEQTGNLFMVGDVKQSIYRFRLAEPNLFLSKYNRFKPTGADSGL
RIDLARNFRSRKEVLDGTNFLFKQFMGVKVGEIDYDEAAELKKGAPYPEETSNPVSVILIDQAGEDDESDADELPEADNI
GDFSREELQQSQLEARVIAKKIKELIENRTEVYNTKTKSSRPVTYRDIVILLRSMTWAPEIMEEFKHQGIPVYANLSTGY
FEATEVSIMMSLLKVIDNPYQDIPLAAVLRSPIVGLNEEELSQIRISNKWGSFYEALMSFCQAKPEADQEALYEKVRPFT
EQLQYWRTKARQGSLSELIWDLFRETKFYDFVGGMPGGKQRQANLRALYDRARQYESTSFRGLFRFLRFIERMIDRGDDL
GAARALGEQEDVVRIMTIHSSKGLEFPVVFIAGLARKFNTMDLKKTFMLDKEFGFAARYVNAEKRISYPTLAQLSFKRKK
KMEMLAEEMRVLYVALTRAKEKLFLIGSVKNAEKSLDRWLSAAATENWLLNDYERASANSYLDWIGPALIRHKDCVVLRA
GINQEKIKLLDHPSCWHVEMIHSEEAGALIEDKRNEREELLEKVAEGKQVPIISKYAERVNEQLSWKYEFHSASVFRSKQ
SVSEIKRQYDLAGEESSTELLRKIRKPLLKRPRFMQEKTLTPAEKGTAMHMVMQQIDLSIPLTEESIRHQVDAMVQKELL
TAEQREGIDPELVLKFFKSDLGQRMLRAEKVMREVPFSLSIPAKETYTDWTGGDEQVLIQGVIDCFFEEEEGIILLDYKT
DRITDRYKGGFEEAKPILESRYSLQINLYARALEQIVRKPVYERYLFFFDGGHLLKLENN

Nucleotide


Download         Length: 3783 bp        

>NTDB_id=122985 BMMGA3_RS04175 WP_034669194.1 834940..838722(+) (addA) [Bacillus methanolicus MGA3]
ATGATCTTGACAAATTCGGTGATAATTCCGCCAAAGCCTGAAAATGTGACATGGACCGATGACCAATGGAAAGCAATAAT
GGCAAAAGACCGGGACATCCTTGTTGCGGCGGCTGCCGGTTCGGGAAAAACGGCCGTTCTCGTTGAGAGAATCATTCGAA
GAATTACTTCCTTGCATGACCCGATTGATGTTGACCAGCTGCTAGTCGTTACTTTTACGAATGCTTCAGCTGCTGAAATG
AGACATCGAATTGGTGAAGCATTAGAAAAAGAGATTAATCAAAACCCAACCTCTGCGCATTTGCGTAAACAATTGAGTCT
GTTGAATAAAGCATCGATTTCAACGCTCCATTCCTTTTGCTTGGAAGTGATAAAAAAATACTATTATTTAATTGATATTG
ATCCAGGGTTCCGGATTGCCGATGAGACGGAAGCTCAACTTATGCGTGACGAGGTTTTGGATGAATTATTTGAAGAAGAA
TACGGTAAAAGTGATAATGAAGCATTCTTTTCACTTGTTGATACGTTTACGAATGACCGGAGCGATGAAGCCCTGCAAGA
TATTATCAGGGAACTTTATGATTTTGCCCGTTCTAATCCTTCTCCTGACCGTTATTTGAAATCGATTATCGAAATGTATG
ATGTTGATGAGAATGGGAAATTAGAAGATCTTCCATTTATGGATGCTCTTTTAGATGACATAGAGATGCAGCTTGAAGGG
GCAAAGCAAATGCTGCTTGAAGGGCTGGAGCTGACGAAGCTTCCCGGAGGACCTGCACCTAGAGCAGAAAATTTTATCGA
TGACTTGCATATTGTTGATACGCTCATTAGCGCAAAAAACGATTCATGGTCAACATTGTATAACGCAATGCAAAACCTGT
CCTTTTCACGTGCTAAAACAGTTCGAGGAGACGAATATGACGGTGATCTGGTCGAAAAAGCCCAAAAATTGAGAGAAAGG
GCGAAAAAAGTTTTGCAGGATTTACAGTCCGAACTTTTCTCAAGAAGGCCTGAATCGTTTTTAAAAGATATGCGAGAAAT
GAAAGGACATATTGAAACTCTCATTTCACTTGTGAAATCTTTTTCGGACCGATTTGAACAAGTGAAAAGAGAAAAGGGAT
TAGTTGATTTTGCTGATTTAGAGCATTACTGTCTTGATATTCTAACCGGAAGCATTGATGAAGACGGAAGAAGAATTCCT
TCCGAAGCAGCACTTTCTTACCGCCGTCTTTTTAAAGAAGTTCTCGTCGATGAGTACCAAGATACAAACATGGTGCAGGA
AGCCATATTGCAGCTCGTCACGAATGAAGAAGAACAGACAGGAAACCTCTTTATGGTAGGGGACGTCAAGCAATCGATCT
ACCGGTTCAGGCTGGCAGAGCCAAATTTATTTTTAAGCAAGTACAACCGTTTTAAGCCGACTGGTGCGGATTCAGGCTTG
AGAATCGATTTAGCCCGGAATTTCCGAAGCCGGAAAGAAGTGCTCGACGGAACGAATTTTCTGTTTAAGCAGTTTATGGG
AGTTAAGGTTGGAGAAATCGATTATGATGAAGCTGCAGAGTTAAAAAAAGGCGCTCCGTATCCTGAAGAAACATCAAACC
CTGTTTCCGTCATCTTGATCGACCAAGCCGGAGAGGATGACGAAAGCGATGCTGATGAATTGCCTGAAGCGGACAACATA
GGAGATTTCAGCCGGGAGGAATTGCAGCAGTCGCAGCTTGAAGCAAGGGTAATAGCAAAAAAAATCAAAGAGCTCATTGA
AAATCGGACTGAAGTATATAACACAAAAACAAAAAGCTCAAGGCCAGTTACTTACAGAGACATTGTAATTCTTCTCAGGT
CCATGACTTGGGCCCCAGAGATCATGGAAGAGTTTAAACACCAAGGTATACCGGTTTATGCAAATTTATCAACTGGATAT
TTTGAAGCAACAGAAGTATCAATTATGATGTCTTTGTTAAAAGTCATTGACAATCCTTATCAAGATATTCCGCTTGCAGC
CGTTTTACGCTCTCCGATTGTAGGATTAAACGAAGAAGAATTGTCGCAAATACGTATTTCAAATAAGTGGGGATCGTTCT
ATGAGGCTCTGATGTCTTTTTGTCAGGCAAAGCCGGAAGCAGATCAAGAAGCACTCTATGAAAAAGTCAGGCCGTTCACA
GAGCAGCTTCAATATTGGAGAACAAAAGCCCGTCAAGGATCATTATCTGAATTGATTTGGGATTTATTTAGGGAAACGAA
ATTTTATGATTTTGTCGGAGGGATGCCAGGCGGAAAACAGCGACAGGCCAACCTTAGAGCGTTATATGACAGGGCACGGC
AATATGAATCGACATCATTTAGAGGTTTGTTTCGCTTTCTCCGGTTTATAGAGCGAATGATCGACCGTGGCGATGACCTG
GGAGCAGCCCGTGCACTGGGAGAACAAGAAGATGTTGTAAGAATTATGACGATTCACAGCAGTAAAGGTCTTGAATTCCC
TGTCGTTTTTATTGCCGGTTTAGCGCGAAAATTCAATACAATGGATCTGAAAAAAACATTCATGCTGGATAAGGAATTTG
GATTCGCTGCCAGGTATGTAAATGCTGAAAAACGGATATCATACCCAACGCTTGCGCAGCTTTCATTTAAGCGTAAAAAG
AAAATGGAAATGCTGGCAGAGGAAATGAGAGTGTTATATGTTGCTTTAACCCGTGCAAAAGAGAAGCTGTTTTTGATAGG
ATCGGTAAAAAATGCTGAAAAATCATTGGACAGATGGCTCTCGGCAGCTGCCACCGAAAACTGGCTATTAAATGATTATG
AGCGGGCGAGTGCAAATAGCTATTTGGATTGGATCGGCCCTGCGCTTATTAGACATAAGGATTGTGTAGTTTTGCGAGCT
GGCATTAATCAGGAAAAAATAAAACTATTAGACCACCCTTCATGCTGGCATGTTGAAATGATTCATAGTGAAGAAGCTGG
AGCATTGATTGAAGATAAAAGAAACGAAAGAGAAGAATTGTTGGAAAAAGTAGCTGAAGGCAAACAGGTTCCAATTATTT
CTAAATATGCAGAACGGGTAAACGAACAGCTTTCATGGAAATATGAATTTCATAGTGCTTCTGTGTTCCGATCCAAGCAA
TCTGTTTCCGAGATTAAGAGGCAGTATGATCTTGCCGGTGAAGAGAGCAGTACGGAACTGCTTCGGAAAATAAGAAAGCC
TCTTTTAAAGCGGCCAAGATTTATGCAAGAAAAAACGTTAACACCAGCAGAAAAAGGAACGGCAATGCATATGGTAATGC
AGCAGATTGATCTGTCGATTCCTTTAACGGAAGAATCGATCCGTCATCAGGTAGATGCCATGGTACAGAAAGAACTCCTT
ACCGCGGAGCAAAGAGAAGGAATCGATCCTGAACTTGTTTTGAAATTTTTTAAGAGCGATCTTGGGCAAAGGATGCTGCG
TGCAGAAAAGGTTATGCGCGAGGTTCCTTTCAGTCTATCAATTCCGGCAAAAGAAACGTATACCGATTGGACTGGTGGAG
ATGAACAAGTCCTTATACAAGGTGTGATTGACTGTTTCTTTGAAGAAGAGGAAGGAATTATACTCCTTGACTATAAAACA
GATCGTATTACGGATCGTTATAAAGGGGGATTTGAAGAAGCAAAACCAATTCTTGAATCGAGGTACAGCCTTCAAATAAA
CCTTTATGCCCGTGCACTTGAACAAATAGTAAGAAAGCCTGTCTATGAGAGATATTTGTTCTTCTTTGACGGGGGACATT
TATTAAAGCTAGAAAATAATTAA

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(18-472)

UvrD-like helicase C-terminal domain (UvrD_C)

(482-907)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1073-1238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

57.28

99.206

0.568

Multiple sequence alignment