Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   BMMGA3_RS04170 Genome accession   NZ_CP007739
Coordinates   831459..834980 (+) Length   1173 a.a.
NCBI ID   WP_004433478.1    Uniprot ID   -
Organism   Bacillus methanolicus MGA3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 826459..839980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMMGA3_RS04135 (BMMGA3_04220) - 827117..827353 (-) 237 WP_004433464.1 IDEAL domain-containing protein -
  BMMGA3_RS04140 (BMMGA3_04225) - 827545..828123 (+) 579 WP_004433465.1 competence protein ComK -
  BMMGA3_RS04145 (BMMGA3_04230) - 828155..828586 (-) 432 WP_004433467.1 hypothetical protein -
  BMMGA3_RS04155 (BMMGA3_04235) - 828926..829861 (+) 936 WP_034669287.1 LCP family protein -
  BMMGA3_RS04160 (BMMGA3_04240) - 829972..830583 (+) 612 WP_004433472.1 TVP38/TMEM64 family protein -
  BMMGA3_RS04165 (BMMGA3_04245) lepB 830621..831175 (+) 555 WP_004433475.1 signal peptidase I -
  BMMGA3_RS04170 (BMMGA3_04250) addB 831459..834980 (+) 3522 WP_004433478.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BMMGA3_RS04175 (BMMGA3_04255) addA 834940..838722 (+) 3783 WP_034669194.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BMMGA3_RS04180 (BMMGA3_04260) - 838765..838986 (-) 222 WP_004433484.1 spore germination protein -
  BMMGA3_RS04185 (BMMGA3_04265) - 839052..839441 (-) 390 WP_004433488.1 spore germination protein GerPE -
  BMMGA3_RS04190 (BMMGA3_04270) - 839443..839625 (-) 183 WP_004433491.1 hypothetical protein -

Sequence

Protein


Download         Length: 1173 a.a.        Molecular weight: 135403.86 Da        Isoelectric Point: 6.7690

>NTDB_id=122984 BMMGA3_RS04170 WP_004433478.1 831459..834980(+) (addB) [Bacillus methanolicus MGA3]
MAVRLITGRSGSGKTEYCLKEIREKLQQNPKGNPIIYLVPEQMTFLSEYKLVTTPNLGGMIRAQVFSFTRLAWRILQETG
GISRYHLNSVGINMLIRKIIEDQKDELKIFQRAADKSGFIQQMEQILTEFKRYCVTPGELLEKQSAFWPDEEQNYKDLKD
KLHDLELIYSRFEDELFGKYVDSEDYLRLLSEKIASSKYLKEADIYIDGFYTYTPQEYMIIEKFIKHCKRVTITLTSDKL
YKENAPDELHLFRLAGETSLTIYEIIKTNGIELEEVHLEEQLRWTNASLRHLESEFDTRPAVPYQGTPEISICQAVNRRA
EIEGVARKIKYLVREKGYRFRDIALLVRNGSDYYDILETIFDDHEIPYFIDQKRTMLNHPLIELIRSSLEVINGHWRYEP
VFRAIKTELMFPVKSNVFKLRQQMDRLENYVLAYGIQGDKWTKKERWIYRRIRGLEFEAVAKTDAEKNIEQELNELRAMV
TAPLLRMARRLKKAETGRDLCEAVYLFLEELDIPAKLEQWKLAAEVNGELVKAREHDQAWNAVMELLDQFVEMLGEEKIT
LKQFASILDAGMESLRFSLVPPAIDQVLVADLERSRLSDIKAAFVIGLNEGVFPAKMGEEGILADEDREILLSKGLKIAP
GSRTRLLDENFLAYKAFVTPSEHLYVSYPLANEEGKALMPSSYIKRITDLFPNARMHFFVADPSELSEEEQLEFISNETT
ALSYLTGQLQLKKRNYPVYDLWWDVYNYYINSDRLRPAAIKALSGLFYENRTKKLSEQVTKKLYGDHIQASVSRMELFHS
CPFSHFARHGLKLAERQVFRLEAPDIGKLFHAALKYIAETVTQKNLSWANMTKDQCEILAREAVEKLAPKLQNEILLSTN
RHQYIKRKLEKIISRASFVLSEHAKASGFSPIGLEVAFGPKEKLPPLSFSLKNGTKMELVGRIDRVDKAEDTSGVFLRVI
DYKSSEKDLNIGEVYYGLALQMLTYLDIIITHSDNLIGTKADPAGVLYFHVHNPIIHSSKVLTLDEIEKEIMKSFRMKGL
LLGEQNVIQLMDKTLESGDSQIVSAGMKKDGTLKKSSKVATREEFEALRRYVRKMYEKTGNAITEGIVDLSPYKMKNKTP
CTFCPYKAVCQFDQSLESNEFRILTPYKKDDVLDLIRKEVDDLDKFGDNSAKA

Nucleotide


Download         Length: 3522 bp        

>NTDB_id=122984 BMMGA3_RS04170 WP_004433478.1 831459..834980(+) (addB) [Bacillus methanolicus MGA3]
ATGGCTGTAAGACTCATAACAGGGAGATCAGGAAGCGGCAAAACTGAATATTGTTTAAAGGAAATACGGGAGAAACTGCA
GCAGAATCCGAAAGGAAACCCGATTATTTATCTCGTTCCTGAGCAGATGACATTTTTATCTGAATACAAACTTGTCACTA
CCCCTAATCTGGGCGGCATGATTCGTGCCCAAGTATTCAGTTTTACCCGTTTGGCCTGGAGAATTCTTCAGGAAACAGGC
GGGATCAGCCGTTATCATCTAAACAGTGTTGGGATTAACATGCTCATCAGAAAAATTATTGAAGATCAGAAGGATGAGTT
GAAAATCTTCCAACGGGCAGCTGATAAATCCGGATTTATCCAGCAAATGGAACAAATACTGACGGAATTTAAGCGATATT
GTGTAACACCAGGAGAACTTTTGGAGAAACAATCCGCGTTTTGGCCGGATGAGGAACAAAATTACAAGGATCTAAAGGAT
AAGCTTCACGATCTTGAATTAATTTACAGCCGATTTGAGGATGAACTGTTTGGGAAATATGTAGATTCCGAGGATTACCT
CAGGCTTCTTTCTGAAAAAATCGCTTCATCCAAGTATTTAAAAGAAGCAGATATTTATATCGATGGTTTTTACACTTACA
CCCCGCAGGAGTATATGATTATCGAAAAATTTATAAAGCATTGCAAGCGGGTTACGATTACTTTAACAAGTGACAAATTG
TATAAAGAAAACGCTCCTGATGAGCTTCATCTTTTTAGATTGGCCGGTGAAACGAGCTTGACGATTTATGAAATCATCAA
AACAAATGGGATCGAATTAGAAGAAGTTCATCTTGAGGAGCAGCTGAGGTGGACAAACGCTTCATTGCGCCATTTAGAAT
CAGAATTTGATACAAGGCCTGCCGTACCTTATCAGGGCACACCTGAGATTTCGATTTGCCAAGCGGTTAACAGGCGGGCG
GAAATAGAAGGAGTTGCCCGAAAGATCAAGTATTTAGTGAGAGAAAAAGGATACCGGTTCCGGGATATCGCGTTGTTGGT
AAGGAATGGAAGTGATTATTACGATATTTTAGAGACAATTTTTGATGATCATGAAATACCATATTTTATTGATCAGAAAC
GAACAATGTTGAACCATCCGCTCATTGAGTTAATACGCTCCAGTCTGGAAGTCATTAATGGCCATTGGCGTTATGAACCG
GTATTTCGCGCCATAAAAACAGAGCTAATGTTTCCTGTAAAATCCAATGTTTTTAAGTTGCGCCAGCAGATGGACAGGCT
TGAAAATTACGTTCTCGCTTACGGAATTCAAGGAGATAAATGGACAAAAAAAGAGCGCTGGATCTACCGTAGAATAAGAG
GGCTTGAGTTTGAAGCAGTTGCAAAGACCGATGCAGAAAAGAACATTGAACAAGAACTAAATGAGCTCAGGGCTATGGTA
ACTGCCCCGCTTCTTCGGATGGCCCGCCGTCTTAAAAAAGCTGAAACTGGAAGAGATTTATGCGAAGCTGTCTATTTATT
TTTGGAAGAATTAGATATCCCCGCAAAACTTGAACAATGGAAGCTTGCTGCTGAAGTAAATGGCGAGCTAGTAAAAGCCC
GGGAGCATGATCAAGCCTGGAACGCCGTAATGGAGTTGTTGGATCAGTTTGTTGAAATGCTTGGTGAGGAAAAAATTACA
CTTAAGCAGTTTGCATCTATTCTTGATGCCGGGATGGAGTCATTGCGATTTTCACTTGTTCCTCCTGCTATCGACCAAGT
TTTAGTTGCAGATTTGGAGAGATCAAGGCTGTCAGATATTAAAGCAGCTTTTGTCATCGGTTTAAATGAAGGGGTTTTTC
CTGCAAAAATGGGTGAGGAAGGAATTCTAGCTGACGAAGACCGTGAGATTTTGCTGTCAAAGGGATTGAAAATAGCGCCT
GGAAGCCGTACGCGCCTTTTGGATGAAAACTTTCTCGCCTATAAAGCATTTGTTACTCCTTCCGAGCACCTTTATGTAAG
TTATCCTTTGGCAAATGAAGAAGGGAAAGCATTGATGCCGTCTTCATATATTAAAAGAATAACGGATCTGTTTCCAAATG
CACGCATGCATTTTTTTGTTGCCGATCCATCAGAGTTATCGGAAGAAGAGCAGCTTGAATTTATTTCAAATGAAACGACT
GCCCTGTCTTATTTGACCGGACAACTCCAACTGAAAAAACGCAATTATCCGGTTTATGATTTATGGTGGGATGTTTATAA
CTATTATATAAATAGTGACAGATTAAGGCCGGCTGCAATTAAAGCTCTGTCTGGCTTATTCTATGAAAACCGGACGAAAA
AGTTATCAGAACAGGTAACTAAAAAATTATACGGAGATCATATTCAAGCAAGCGTGTCAAGGATGGAGCTCTTTCACAGC
TGTCCGTTCTCCCATTTTGCTCGACATGGTTTAAAACTGGCGGAACGGCAGGTATTTCGTCTTGAAGCACCTGATATCGG
TAAATTATTCCATGCTGCATTAAAGTATATCGCAGAAACAGTGACGCAAAAAAATTTGTCCTGGGCAAACATGACAAAGG
ATCAATGTGAAATTCTTGCGAGAGAAGCGGTTGAAAAACTGGCTCCCAAATTGCAAAATGAAATCCTGTTGAGTACAAAT
CGCCATCAATACATTAAAAGAAAACTTGAAAAAATTATTAGCAGGGCTTCGTTTGTATTAAGTGAACATGCAAAGGCAAG
CGGTTTTTCCCCGATAGGCTTGGAAGTGGCTTTCGGTCCGAAGGAAAAACTGCCGCCTCTATCATTCTCTTTAAAAAATG
GAACGAAAATGGAATTGGTTGGCCGGATTGACCGGGTTGATAAAGCAGAAGACACGAGCGGCGTATTTTTAAGAGTTATA
GATTACAAGTCGAGCGAAAAAGATTTAAATATTGGGGAAGTTTACTACGGTCTTGCACTGCAAATGCTTACGTACCTTGA
TATTATCATTACTCATTCGGATAATTTAATTGGTACGAAAGCAGACCCTGCGGGAGTCCTATATTTCCATGTTCATAATC
CGATCATCCATTCTTCAAAAGTGCTGACACTTGATGAAATTGAAAAAGAGATTATGAAAAGCTTTCGAATGAAAGGGCTT
CTTCTCGGGGAGCAAAATGTAATTCAATTAATGGACAAAACGCTGGAATCCGGAGACTCGCAAATTGTCTCCGCCGGAAT
GAAAAAGGATGGAACGTTGAAAAAGTCTTCGAAAGTGGCGACAAGAGAAGAGTTCGAAGCATTAAGGCGTTATGTCCGGA
AAATGTATGAGAAAACAGGCAATGCCATTACAGAGGGAATAGTCGATCTTTCTCCTTATAAAATGAAGAACAAAACTCCG
TGCACGTTTTGTCCGTATAAAGCGGTGTGCCAGTTCGATCAATCACTAGAAAGCAATGAGTTTCGTATTTTAACGCCATA
CAAAAAAGATGATGTTTTGGATTTAATTCGAAAGGAGGTAGATGATCTTGACAAATTCGGTGATAATTCCGCCAAAGCCT
GA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(285-627)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

51.976

99.233

0.516

Multiple sequence alignment