Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BMMGA3_RS00565 Genome accession   NZ_CP007739
Coordinates   108496..109875 (+) Length   459 a.a.
NCBI ID   WP_003348667.1    Uniprot ID   I3E460
Organism   Bacillus methanolicus MGA3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 103496..114875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMMGA3_RS00545 (BMMGA3_00535) - 103788..104249 (+) 462 WP_412150597.1 CtsR family transcriptional regulator -
  BMMGA3_RS00550 (BMMGA3_00540) - 104276..104824 (+) 549 WP_003348673.1 UvrB/UvrC motif-containing protein -
  BMMGA3_RS00555 (BMMGA3_00545) - 104827..105891 (+) 1065 WP_003348671.1 protein arginine kinase -
  BMMGA3_RS00560 (BMMGA3_00550) clpC 105914..108358 (+) 2445 WP_003348669.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BMMGA3_RS00565 (BMMGA3_00555) radA 108496..109875 (+) 1380 WP_003348667.1 DNA repair protein RadA Machinery gene
  BMMGA3_RS00570 (BMMGA3_00560) disA 109878..110951 (+) 1074 WP_003348665.1 DNA integrity scanning diadenylate cyclase DisA -
  BMMGA3_RS00575 (BMMGA3_00565) - 111172..112254 (+) 1083 WP_003348663.1 PIN/TRAM domain-containing protein -
  BMMGA3_RS00580 (BMMGA3_00570) ispD 112321..113019 (+) 699 WP_003348661.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BMMGA3_RS00585 (BMMGA3_00575) ispF 113050..113529 (+) 480 WP_003348659.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49834.30 Da        Isoelectric Point: 7.6621

>NTDB_id=122981 BMMGA3_RS00565 WP_003348667.1 108496..109875(+) (radA) [Bacillus methanolicus MGA3]
MAKRKTKFICQKCGYESPKWMGKCPGCGEWNKMVEEVEPASTGRRGAFAYSVGTSSSSKATPITSIETLSEPRIQTDLNE
FNRVLGSGIVRGSLVLIGGDPGIGKSTLLLQVSSQLAAKSYQVLYVSGEESLRQLKLRADRLGVSSENLLVYSETNLEEI
SKTIENVSPSFVIVDSIQTVFHPEITSAPGSVSQIRECTAELMRIAKSKGIAIFIVGHVTKEGSIAGPRILEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSQGAAGSTVVASMEGTRPVLVEIQALISPSS
FGIPRRMATGIDHNRVPLLMAVLEKRVGMLLQNQDAYLKVAGGIKLDEPAIDLAIAISIASSFRGKPTRPTDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPQNNLGGWQGPKGIELVGVSSVSQALQVTLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=122981 BMMGA3_RS00565 WP_003348667.1 108496..109875(+) (radA) [Bacillus methanolicus MGA3]
ATGGCAAAACGAAAAACGAAGTTTATATGCCAGAAATGCGGCTATGAATCGCCGAAATGGATGGGCAAATGCCCGGGCTG
CGGTGAGTGGAACAAAATGGTTGAGGAAGTAGAACCGGCCAGCACAGGGAGAAGAGGGGCCTTCGCATACTCTGTCGGAA
CTTCATCAAGTTCAAAAGCTACGCCGATTACATCAATTGAAACCTTAAGTGAACCGCGAATTCAAACAGATCTAAATGAA
TTCAATAGGGTTCTTGGAAGTGGGATCGTCAGAGGTTCTTTAGTATTAATCGGAGGGGATCCTGGAATAGGAAAATCAAC
ATTGCTTTTACAAGTATCTTCACAGCTTGCTGCCAAAAGTTATCAAGTTTTATATGTATCCGGAGAGGAATCGCTGCGCC
AGCTAAAGCTTAGGGCGGATCGCCTTGGAGTGTCTTCTGAAAATTTGCTTGTGTATTCAGAAACCAATCTTGAAGAAATA
AGCAAAACGATTGAAAATGTTTCACCAAGCTTTGTTATTGTTGACTCAATCCAAACGGTTTTCCACCCCGAAATTACTTC
AGCACCTGGAAGCGTTTCGCAAATAAGAGAGTGTACGGCAGAATTGATGAGAATTGCGAAATCGAAAGGGATTGCTATAT
TTATTGTGGGGCATGTTACAAAAGAAGGATCGATTGCAGGTCCGCGCATTTTGGAACACATGGTTGATACCGTTTTATAT
TTTGAAGGAGAGAGGCACCATACGTACCGCATATTACGCGCAGTCAAAAACAGGTTCGGTTCGACAAATGAAATGGGTAT
TTTTGAGATGAAAGAATTTGGCCTTGAAGAAGTTGAAAATCCGTCGGAAATTTTTCTAGAAGAACGGTCTCAAGGTGCAG
CTGGTTCTACAGTGGTCGCTTCAATGGAAGGAACGAGACCGGTACTTGTGGAAATTCAAGCGCTTATTTCACCTTCGAGC
TTCGGAATCCCAAGGCGGATGGCAACAGGAATTGATCATAACAGGGTTCCGCTTTTAATGGCTGTTCTTGAGAAAAGAGT
CGGAATGCTGCTTCAAAACCAGGATGCGTATTTAAAAGTGGCTGGCGGAATAAAGCTTGACGAGCCAGCTATTGATCTGG
CAATAGCGATAAGCATTGCATCTAGTTTTCGCGGCAAGCCGACAAGACCAACGGATTGTATTATAGGCGAAGTCGGATTG
ACAGGCGAAGTAAGGCGAGTTTCAAGGATTGAGCAAAGGGTGCAGGAAGCAGCGAAACTTGGTTTTGAAAGAGTGATTTT
GCCGCAAAATAATCTCGGAGGATGGCAAGGTCCGAAAGGCATCGAATTGGTCGGAGTTTCATCTGTGAGCCAGGCACTTC
AAGTTACGTTAGGGGGATGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(75-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3E460

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.522

100

0.767
  radA Streptococcus mitis NCTC 12261

60.793

98.911

0.601
  radA Streptococcus pneumoniae Rx1

60.793

98.911

0.601
  radA Streptococcus pneumoniae D39

60.793

98.911

0.601
  radA Streptococcus pneumoniae R6

60.793

98.911

0.601
  radA Streptococcus pneumoniae TIGR4

60.793

98.911

0.601
  radA Streptococcus mitis SK321

60.573

98.911

0.599

Multiple sequence alignment