Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   EP20_RS08350 Genome accession   NZ_CP007657
Coordinates   1683946..1684632 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain V2200     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1678946..1689632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EP20_RS08330 (EP20_007975) rpsT 1679861..1680112 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  EP20_RS08335 (EP20_007980) holA 1680157..1681131 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  EP20_RS08340 (EP20_007985) - 1681188..1683388 (-) 2201 Protein_1611 DNA internalization-related competence protein ComEC/Rec2 -
  EP20_RS08345 (EP20_007990) - 1683393..1683854 (-) 462 WP_000439693.1 ComE operon protein 2 -
  EP20_RS08350 (EP20_007995) comEA 1683946..1684632 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  EP20_RS08355 (EP20_008000) - 1684672..1685388 (-) 717 WP_000084829.1 class I SAM-dependent methyltransferase -
  EP20_RS08360 (EP20_008005) rsfS 1685391..1685744 (-) 354 WP_001088022.1 ribosome silencing factor -
  EP20_RS08365 (EP20_008010) yqeK 1685745..1686329 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EP20_RS08370 (EP20_008015) nadD 1686319..1686888 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  EP20_RS08375 (EP20_008020) yhbY 1686891..1687181 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  EP20_RS08380 (EP20_008025) aroE 1687185..1687991 (-) 807 WP_000666761.1 shikimate dehydrogenase -
  EP20_RS08385 (EP20_008030) yqeH 1688005..1689105 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=122059 EP20_RS08350 WP_001792568.1 1683946..1684632(-) (comEA) [Staphylococcus aureus strain V2200]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=122059 EP20_RS08350 WP_001792568.1 1683946..1684632(-) (comEA) [Staphylococcus aureus strain V2200]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment