Detailed information    

experimental Experimentally validated

Overview

Name   yhxC   Type   Unclear
Locus tag   BSU_10400 Genome accession   NC_000964
Coordinates   1115698..1116555 (+) Length   285 a.a.
NCBI ID   NP_388921.1    Uniprot ID   P40397
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   unknown   
Unclear

Function

The yhxC gene has been identified as a novel player in natural competence and transformation.


Genomic Context

Location: 1110698..1121555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_10370 (BSU10370) bioY 1111925..1112485 (-) 561 NP_388918.1 biotin transporter -
  BSU_10380 (BSU10380) hemAT 1112620..1113918 (-) 1299 NP_388919.1 heme-based dioxygen sensor -
  BSU_10390 (BSU10390) yhfW 1114057..1115586 (-) 1530 NP_388920.1 putative oxidoreductase with Rieske [2Fe-2S] center Unclear
  BSU_10400 (BSU10400) yhxC 1115698..1116555 (+) 858 NP_388921.1 putative oxidoreductase Unclear
  BSU_10410 (BSU10410) yhzC 1116583..1116816 (-) 234 NP_388922.1 hypothetical protein -
  BSU_10420 (BSU10420) comK 1117109..1117687 (+) 579 NP_388923.1 competence transcription factor (CTF) Regulator
  BSU_10430 (BSU10430) yhxD 1117734..1118633 (-) 900 NP_388924.1 putative oxidoreductase -
  BSU_10440 (BSU10440) yhjA 1118850..1119119 (+) 270 NP_388925.1 hypothetical protein -
  BSU_10450 (BSU10450) yhjB 1119162..1120631 (-) 1470 NP_388926.1 putative Na+/metabolite cotransporter -
  BSU_10460 (BSU10460) yhjC 1120628..1120828 (-) 201 NP_388927.1 hypothetical protein -
  BSU_10470 (BSU10470) yhjD 1121036..1121398 (-) 363 NP_388928.1 hypothetical protein -

Sequence

Protein


Download         Length: 285 a.a.        Molecular weight: 30845.20 Da        Isoelectric Point: 7.4284

>NTDB_id=122 BSU_10400 NP_388921.1 1115698..1116555(+) (yhxC) [Bacillus subtilis subsp. subtilis str. 168]
MANQKKKTLPPQHQNQQPGFEYLMDPRPVFDKPKKAKKLEGKTAIITGGDSGIGRAVSVLFAKEGANVVIVYLNEHQDAE
ETKQYVEKEGVKCLLIAGDVGDEAFCNDVVGQASQVFPSIDILVNNAAEQHVQPSIEKITSHQLIRTFQTNIFSMFYLTK
AVLPHLKKGSSIINTASITAYKGNKTLIDYSATKGAIVTFTRSLSQSLVQQGIRVNAVAPGPIWTPLIPASFAAKDVEVF
GSDVPMERPGQPVEVAPSYLYLASDDSTYVTGQTIHVNGGTIVNG

Nucleotide


Download         Length: 858 bp        

>NTDB_id=122 BSU_10400 NP_388921.1 1115698..1116555(+) (yhxC) [Bacillus subtilis subsp. subtilis str. 168]
TTGGCGAATCAAAAAAAGAAAACATTACCGCCTCAGCACCAGAACCAGCAGCCTGGTTTTGAATATCTGATGGACCCGCG
TCCGGTTTTTGATAAGCCGAAGAAAGCGAAAAAATTAGAGGGCAAAACCGCTATTATTACTGGAGGAGACAGCGGGATTG
GACGCGCTGTCTCGGTGTTATTCGCAAAAGAAGGGGCTAATGTGGTCATTGTGTATTTGAATGAACATCAGGACGCCGAG
GAAACAAAGCAGTATGTAGAAAAGGAAGGGGTAAAATGCCTGCTGATTGCAGGAGATGTCGGGGATGAAGCGTTTTGCAA
TGATGTGGTCGGGCAGGCAAGCCAAGTGTTTCCATCCATTGATATATTGGTTAACAATGCAGCTGAGCAGCATGTCCAGC
CCAGCATTGAAAAAATCACCAGCCACCAGCTGATCAGAACCTTCCAAACAAACATTTTTTCCATGTTTTACTTAACAAAG
GCAGTGCTGCCTCATTTAAAAAAAGGGAGCTCTATTATTAATACCGCTTCAATTACCGCCTATAAAGGCAATAAAACGCT
GATCGATTATTCAGCGACAAAAGGCGCGATCGTTACGTTTACAAGGTCCCTTTCCCAGTCGCTTGTTCAGCAGGGCATAC
GGGTCAATGCAGTGGCACCGGGTCCCATTTGGACACCGCTTATCCCGGCCAGCTTTGCCGCAAAAGACGTGGAAGTGTTT
GGTTCAGACGTGCCGATGGAACGCCCGGGGCAGCCGGTCGAAGTGGCGCCAAGCTATCTATACCTTGCCAGCGACGATTC
CACATACGTCACAGGGCAGACGATTCACGTCAATGGCGGAACAATTGTGAACGGATAA

Domains

Predicted by InterproScan.

Enoyl-(Acyl carrier protein) reductase (adh_short_C2)

(51-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P40397

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Mirjam Boonstra et al. (2020) Analyses of competent and non-competent subpopulations of Bacillus subtilis reveal yhfW, yhxC and ncRNAs as novel players in competence. Environmental Microbiology 22(6):2312-2328. [PMID: 32249531]