Detailed information    

experimental Experimentally validated

Overview

Name   yhfW   Type   Unclear
Locus tag   BSU_10390 Genome accession   NC_000964
Coordinates   1114057..1115586 (-) Length   509 a.a.
NCBI ID   NP_388920.1    Uniprot ID   O07622
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   unknown   
Unclear

Function

The yhfW gene has been identified as a novel player in natural competence and transformation. Recent studies have shown that yhfW is involved in the regulation of competence development, although its exact mechanism is still under investigation.


Genomic Context

Location: 1109057..1120586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_10350 (BSU10350) yhfS 1109388..1110482 (-) 1095 NP_388916.1 acetyl-CoA C-acetyltransferase -
  BSU_10360 (BSU10360) yhfT 1110479..1111918 (-) 1440 NP_388917.1 putative long-chain fatty-acid-CoA ligase (proofreading for biotin synthesis) -
  BSU_10370 (BSU10370) bioY 1111925..1112485 (-) 561 NP_388918.1 biotin transporter -
  BSU_10380 (BSU10380) hemAT 1112620..1113918 (-) 1299 NP_388919.1 heme-based dioxygen sensor -
  BSU_10390 (BSU10390) yhfW 1114057..1115586 (-) 1530 NP_388920.1 putative oxidoreductase with Rieske [2Fe-2S] center Unclear
  BSU_10400 (BSU10400) yhxC 1115698..1116555 (+) 858 NP_388921.1 putative oxidoreductase Unclear
  BSU_10410 (BSU10410) yhzC 1116583..1116816 (-) 234 NP_388922.1 hypothetical protein -
  BSU_10420 (BSU10420) comK 1117109..1117687 (+) 579 NP_388923.1 competence transcription factor (CTF) Regulator
  BSU_10430 (BSU10430) yhxD 1117734..1118633 (-) 900 NP_388924.1 putative oxidoreductase -
  BSU_10440 (BSU10440) yhjA 1118850..1119119 (+) 270 NP_388925.1 hypothetical protein -

Sequence

Protein


Download         Length: 509 a.a.        Molecular weight: 57109.88 Da        Isoelectric Point: 6.0097

>NTDB_id=121 BSU_10390 NP_388920.1 1114057..1115586(-) (yhfW) [Bacillus subtilis subsp. subtilis str. 168]
MANQHFAKEAPETYWKTSTDLPSFPALQEDTECDVTIIGGGITGITTAYELTKRGFRVVLIEANQVLNGTTAHTTAKVTA
QHDMIYDEFIRHFGLNHARLYYEANQNAIDYIKGIVDEHQIDCEWIEQDAYLYTANENAVQKIHTEHEAYTKLGIERDLI
KDLPIPLGSKLALVMKNQAQFHPLQYLKALLEQIVQKGGRIYEETVALDIKKGERPEVVTKSRHAIKSRFIICCSHFPFY
DGGGLYAARMYSDRSYVLAIKPKIEYPEGMYLSIDQPSVALRYTVVNGEKLILFSGVSHKTGQGKAMSTHYETLRQLAES
TIGIESIPYYWSTQDLVTIDKIPFIGPMSENEDNILVATGFKKWGMTSSAVAATLLSDLVEKKENPYESIFTPSRFHLNP
GLQKVISYNADVAKHLIKGKLEKPDVQFEDISPGEGKAVTINGRRAGAFRDETGCLHLVDTTCTHLGCEVEWNDSEHTWD
CPCHGSRFKPSGEVVEGPAIKPLKQIDLD

Nucleotide


Download         Length: 1530 bp        

>NTDB_id=121 BSU_10390 NP_388920.1 1114057..1115586(-) (yhfW) [Bacillus subtilis subsp. subtilis str. 168]
TTGGCTAATCAACATTTTGCAAAGGAAGCACCTGAAACATACTGGAAAACATCAACTGACCTGCCTTCTTTTCCTGCGCT
TCAAGAGGACACTGAATGTGACGTCACCATTATCGGAGGCGGGATTACGGGAATCACAACGGCATATGAACTTACGAAGA
GAGGCTTCCGTGTCGTTTTGATTGAAGCAAATCAGGTTCTTAACGGAACCACTGCGCATACAACTGCTAAAGTTACCGCA
CAGCATGACATGATATACGACGAATTTATCCGCCATTTCGGATTAAACCATGCGAGGCTTTATTATGAAGCAAACCAAAA
CGCAATTGATTACATAAAAGGCATTGTGGATGAACATCAAATTGATTGTGAATGGATCGAGCAAGACGCATATCTTTATA
CGGCAAATGAAAACGCTGTTCAAAAAATCCACACCGAGCATGAGGCATACACAAAGCTCGGAATTGAAAGAGACCTTATC
AAGGATCTTCCAATTCCCTTAGGTTCTAAGCTGGCGCTTGTCATGAAGAATCAAGCTCAATTTCATCCGCTTCAATACTT
AAAAGCGCTTCTGGAGCAAATTGTTCAAAAAGGCGGCCGAATTTATGAAGAGACCGTTGCGCTTGATATCAAAAAAGGCG
AAAGGCCCGAAGTGGTAACAAAAAGCCGGCACGCCATTAAGAGCCGCTTTATCATATGCTGTTCCCACTTCCCTTTTTAT
GACGGAGGCGGATTATATGCTGCGAGAATGTACTCCGACAGATCTTATGTCCTTGCCATCAAGCCGAAGATCGAGTATCC
CGAGGGCATGTATTTGAGTATCGATCAGCCGTCAGTGGCGCTCCGCTATACGGTTGTAAACGGTGAAAAGCTGATTCTCT
TCAGCGGAGTAAGCCATAAAACAGGCCAGGGAAAAGCCATGTCTACACATTATGAAACACTGAGACAACTGGCCGAAAGC
ACCATCGGCATCGAAAGCATTCCATATTATTGGTCCACCCAAGATCTAGTGACGATCGACAAAATCCCGTTTATCGGGCC
GATGTCGGAAAATGAGGACAATATTCTAGTAGCGACCGGGTTCAAAAAATGGGGCATGACGTCCTCAGCAGTCGCAGCCA
CTTTATTGTCAGACCTTGTGGAGAAAAAAGAAAACCCATACGAAAGCATTTTCACGCCGTCCCGTTTCCATCTGAATCCC
GGGTTGCAGAAAGTGATTTCCTACAATGCAGATGTCGCCAAGCATTTGATAAAAGGAAAGCTTGAAAAGCCTGATGTCCA
ATTTGAAGACATTTCGCCAGGAGAAGGGAAAGCCGTCACCATAAACGGCAGGCGAGCGGGAGCATTTCGTGATGAGACAG
GCTGCCTCCATCTCGTTGATACGACTTGTACCCATTTAGGCTGCGAGGTCGAGTGGAACGACAGTGAGCATACGTGGGAT
TGTCCGTGCCACGGATCACGCTTTAAGCCATCTGGCGAAGTTGTAGAGGGTCCGGCCATTAAACCATTAAAACAAATAGA
TCTCGATTAA

Domains

Predicted by InterproScan.

FAD dependent oxidoreductase (DAO)

(34-379)

Rieske [2Fe-2S] domain (Rieske)

(429-506)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O07622

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Mirjam Boonstra et al. (2020) Analyses of competent and non-competent subpopulations of Bacillus subtilis reveal yhfW, yhxC and ncRNAs as novel players in competence. Environmental Microbiology 22(6):2312-2328. [PMID: 32249531]