Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   MA06_RS20560 Genome accession   NZ_CP007626
Coordinates   3661348..3665073 (+) Length   1241 a.a.
NCBI ID   WP_040119299.1    Uniprot ID   -
Organism   Bacillus pseudomycoides strain 219298     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3656348..3670073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MA06_RS20545 (DJ92_3752) - 3656498..3657091 (+) 594 WP_006093908.1 TVP38/TMEM64 family protein -
  MA06_RS20550 (DJ92_3753) lepB 3657150..3657713 (+) 564 WP_018783054.1 signal peptidase I -
  MA06_RS20555 (DJ92_3754) addB 3657839..3661351 (+) 3513 WP_040119298.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MA06_RS20560 (DJ92_3755) addA 3661348..3665073 (+) 3726 WP_040119299.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MA06_RS20565 (DJ92_3756) - 3665151..3665444 (+) 294 WP_006093912.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  MA06_RS20570 (DJ92_3757) gerPF 3665530..3665745 (-) 216 WP_003195867.1 spore germination protein GerPF -
  MA06_RS20575 (DJ92_3758) - 3665791..3666177 (-) 387 WP_006093913.1 spore germination protein GerPE -
  MA06_RS20580 (DJ92_3759) - 3666190..3666384 (-) 195 WP_018766557.1 hypothetical protein -
  MA06_RS20585 (DJ92_3760) - 3666391..3667005 (-) 615 WP_006093914.1 spore germination protein GerPC -
  MA06_RS20590 (DJ92_3761) - 3667089..3667280 (-) 192 WP_006093915.1 spore germination protein GerPB -
  MA06_RS20595 (DJ92_3762) - 3667297..3667518 (-) 222 WP_006093916.1 spore germination protein -
  MA06_RS20600 (DJ92_3763) - 3667654..3667833 (-) 180 WP_018783051.1 aspartyl-phosphate phosphatase Spo0E family protein -
  MA06_RS20605 (DJ92_3764) - 3668075..3668974 (+) 900 WP_018783050.1 fumarylacetoacetate hydrolase family protein -
  MA06_RS20610 (DJ92_3765) - 3669048..3669449 (+) 402 WP_006093918.1 HXXEE domain-containing protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 143291.71 Da        Isoelectric Point: 4.9577

>NTDB_id=121781 MA06_RS20560 WP_040119299.1 3661348..3665073(+) (addA) [Bacillus pseudomycoides strain 219298]
MIENWPQKPEGSQWTDDQWKAVVRDGRDILVAAAAGSGKTAVLVERIIRKIINAENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSGHVRKQLSLLNKASISTIHSFCLQVIRTYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
GNDIFFELVDRYTSDRSDDDLQRMILALHTESRAHPDPEKWLDKLVEAYDVEGKSIEDLSYATYLLEDVKFQLETAEKHI
RKAIELAMLPDGPAPRIETLQADLVLLGTLSSAAHQSWTSVYEAMQNVSWQTLKRIKKSDYNEEIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFKDMHPILEKLVQIVKVFTERFQTIKRDKGMVDFTDLEHFCLQILSERTNDGELRPSLVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDFEQEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTPEGIDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGETVGEIDYDADAELKLGANYPEGEDVAAELLCIHQSEGEESVEGEDGVEVEKAQLEARL
MAQRIKAMVDSGYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYAELATGYFEATEVTVMMNVFRVID
NPMQDIPLAAVLRSPIVGLNDEELAMLRAHEKKGSFYEVMRSFLQGAPLEEEAELHGKLQWFYNLLQVWREFARQQSLSD
LIWKVYRETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMT
IHKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTISQLAIKRKMKMELIAEEMRVLYVALT
RAKEKLILIGTVKDKEKEIEKWLDSREHTDWLLPDYVRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPNEIYEYDASW
KVEVVDGSTLLAPEPVQEEKQELLEALREKKPVPLESERKEEVYARLTWEYAYEGATAHRAKQSVTEIKRNYQSEDGSDN
AFIKKLRTPIKTRPRFMEKKGLTYAERGTAVHAVMQHVNLQKPITLETVQEQIAEMVNKELLTFEQAEEIAIDRIVAFFD
TDLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQNQSRESVLVQGVIDCMIEEEDGVVLIDFKTDTIEGKFSGGFDQAKPI
LEERYKVQLSLYAKALEKSLHHPVKEKCLYFFDGNHIVHVD

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=121781 MA06_RS20560 WP_040119299.1 3661348..3665073(+) (addA) [Bacillus pseudomycoides strain 219298]
ATGATAGAGAATTGGCCACAGAAGCCTGAAGGTAGTCAATGGACAGATGATCAATGGAAGGCAGTCGTCAGAGATGGCCG
TGATATTTTAGTAGCGGCGGCAGCGGGATCGGGAAAGACAGCTGTACTTGTAGAAAGAATTATTCGCAAAATTATTAATG
CTGAGAATCCAGTAGATGTGGATCGCTTGCTTGTCGTAACATTTACAAATGCAGCGGCACAAGAGATGAAAAACAGAATT
GGTGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGATCAGGGCATGTTCGGAAACAGCTCAGCTTATTAAATAAAGC
ATCCATTTCAACCATTCACTCGTTCTGTTTACAAGTGATTCGAACGTACTATTATATGCTCGACGTTGATCCACGTTTTC
GCATTGCTAATCAGACGGAAAATGAATTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAGGAATATGGAATCGAA
GGAAATGACATTTTCTTTGAACTAGTTGATCGATATACAAGCGATCGTAGTGATGATGACCTGCAGCGTATGATTTTAGC
GCTTCATACGGAATCGAGGGCACATCCAGATCCAGAGAAGTGGCTGGATAAGCTTGTCGAAGCATATGATGTGGAAGGGA
AATCAATTGAAGACTTATCGTATGCAACTTATTTATTAGAAGATGTGAAGTTTCAGCTGGAAACTGCTGAAAAGCATATT
CGAAAAGCAATTGAGTTAGCTATGCTTCCGGATGGGCCAGCGCCTCGCATTGAGACATTACAAGCGGACCTTGTGTTACT
CGGGACGTTATCTAGTGCAGCACATCAGTCTTGGACAAGTGTATATGAAGCGATGCAAAATGTTTCGTGGCAAACGTTAA
AGCGTATTAAAAAGAGTGATTACAATGAAGAGATTGTCAAACAAGTAGATTCACTTAGAAATAAAGCGAAAGATGAAGTG
AAGAAGCTACAAGAAGAGTTGTTTAGCCGGAAACCAGAAAGCTTTTTACGTGATTTCAAGGATATGCACCCTATACTAGA
AAAGCTTGTTCAGATTGTAAAAGTATTTACTGAGCGTTTTCAAACGATAAAGCGTGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTTTGCTTACAAATTTTAAGTGAACGTACGAACGATGGTGAGCTTCGTCCATCACTAGTTGCGCTTCAA
TATCGGAATAAATTTGCCGAAGTACTTGTCGATGAATATCAAGATACGAACTTTGTGCAGGAGTCAATTATTAAGTTTGT
AACGAAAGACTTTGAACAAGAAGGTAATCTGTTTATGGTCGGCGACGTAAAACAGTCGATATACCGATTCCGATTAGCAG
AGCCTGGCTTATTTCTAGGAAAGTATAAACGTTTTACACCTGAAGGAATAGACGGCGGGATGAAGATTGATTTAGCCAAA
AACTTCCGCAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAACTGTCGGGGAAAT
CGATTACGATGCTGATGCTGAACTAAAGTTAGGTGCGAACTACCCAGAAGGAGAAGATGTAGCAGCCGAACTGTTATGTA
TTCATCAGTCTGAGGGAGAAGAATCGGTAGAGGGAGAAGACGGTGTAGAAGTAGAAAAAGCACAGCTTGAAGCACGTTTA
ATGGCGCAGCGAATTAAAGCGATGGTTGATTCAGGTTATGAAGTATATGATCGTAAAACAGATAGCATGCGCCCTGTGAA
ATACCGTGACTTTGTCATTTTACTTCGCTCGATGCCGTGGGCTCCGCAAATTATGGAAGAGTTAAAATTACAAGGCATTC
CTGTTTACGCTGAGCTCGCGACAGGTTATTTTGAAGCGACAGAAGTGACCGTTATGATGAATGTGTTTCGGGTAATTGAT
AATCCGATGCAAGATATTCCGCTTGCTGCTGTGCTTCGTTCCCCGATTGTTGGGTTAAATGATGAAGAGCTTGCGATGTT
ACGTGCTCATGAGAAAAAAGGTTCGTTTTATGAAGTGATGCGTTCCTTCTTACAAGGCGCGCCACTTGAAGAAGAGGCAG
AATTACATGGGAAATTACAATGGTTCTATAATCTCTTGCAAGTGTGGCGTGAATTTGCGCGCCAGCAGTCTCTTTCTGAT
TTAATTTGGAAAGTATATCGTGAGACGGGTTATTACGATTTCGTTGGTGGTCTTCCAGCGGGAAAACAGCGTCAAGCTAA
CTTACGCGTACTATATGATCGCGCAAGGCAGTACGAAGCAACATCTTTCCGCGGGTTATTCCGTTTCCTTCGCTTTATTG
AACGTATTTTAGAGCGCGGGGACGACATGGGAACAGCGAGAGCGCTTGGCGAACAAGAAGATGTGGTTCGAATTATGACG
ATTCATAAAAGTAAAGGGTTAGAGTTCCCAGTTGTATTTGTAGCGGGGCTTGGACGCCGTTTTAATACACAAGATTTAAT
GAAACGATTCTTATTACACAAAGATTTCGGTTTTGGATCACAGTTTATTGATCCTCGTAAACGAATTAAATATACAACGA
TATCACAACTAGCCATTAAACGTAAAATGAAGATGGAACTAATTGCGGAAGAGATGCGCGTATTATATGTTGCTCTAACG
CGTGCAAAAGAAAAACTAATTTTAATTGGTACGGTGAAAGATAAAGAAAAAGAAATTGAAAAGTGGCTTGACTCGCGTGA
ACATACAGATTGGTTGTTACCGGATTACGTTCGTGCAGGAGCATCTTGTTACTTAGATTGGATTGCACCGTCACTATATA
GACATCGTGATAGCGAAATGCTTCTTGAACTTGGACAAGGAAATATACCAAATGAAATTTATGAATACGATGCGAGCTGG
AAAGTGGAAGTGGTTGATGGTAGCACTTTACTTGCACCGGAACCAGTACAAGAAGAAAAGCAAGAATTGTTAGAAGCGCT
TCGTGAGAAAAAACCTGTTCCTCTAGAAAGTGAACGAAAAGAAGAGGTGTATGCTCGTTTAACTTGGGAATATGCGTATG
AAGGTGCAACAGCGCATCGGGCGAAGCAGTCTGTTACTGAGATAAAGCGGAATTATCAATCGGAAGATGGAAGTGATAAT
GCCTTTATTAAAAAACTTCGTACTCCTATAAAAACGCGTCCTCGTTTTATGGAGAAAAAGGGGTTAACTTATGCTGAACG
GGGGACAGCTGTTCACGCCGTAATGCAACATGTTAATTTACAAAAACCGATCACGCTTGAAACCGTTCAAGAGCAAATTG
CGGAAATGGTAAATAAAGAATTACTAACATTTGAGCAAGCAGAAGAAATTGCGATTGATCGAATTGTAGCATTTTTTGAT
ACGGATCTTGGTAAGCGTGTGTTAGCTGCGAAAAGTGTAGAACGTGAAGTGCCGTTTACGATGATGCTTTCAGCGGAAGA
AGCGTATCAAGATTGGCAAAATCAGAGCAGAGAATCTGTACTTGTCCAAGGGGTTATCGATTGTATGATAGAAGAGGAAG
ACGGAGTTGTATTAATCGATTTTAAAACAGATACAATTGAAGGAAAGTTCTCAGGTGGATTCGATCAAGCAAAGCCGATT
TTAGAAGAACGTTATAAAGTACAGCTTTCATTATATGCGAAAGCGTTAGAGAAAAGTTTACATCATCCAGTGAAAGAAAA
GTGTTTATATTTCTTTGATGGAAATCACATAGTACATGTTGATTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-891)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1062-1232)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.569

100

0.538

Multiple sequence alignment