Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MA06_RS15385 Genome accession   NZ_CP007626
Coordinates   2566965..2568341 (+) Length   458 a.a.
NCBI ID   WP_003194303.1    Uniprot ID   A0AAJ3RAI6
Organism   Bacillus pseudomycoides strain 219298     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2561965..2573341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MA06_RS15365 (DJ92_2686) - 2562185..2562646 (+) 462 WP_006093087.1 CtsR family transcriptional regulator -
  MA06_RS15370 (DJ92_2687) - 2562795..2563343 (+) 549 WP_006093088.1 UvrB/UvrC motif-containing protein -
  MA06_RS15375 (DJ92_2688) - 2563348..2564412 (+) 1065 WP_006093089.1 protein arginine kinase -
  MA06_RS15380 (DJ92_2689) clpC 2564435..2566870 (+) 2436 WP_006093090.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MA06_RS15385 (DJ92_2690) radA 2566965..2568341 (+) 1377 WP_003194303.1 DNA repair protein RadA Machinery gene
  MA06_RS15390 (DJ92_2691) disA 2568345..2569418 (+) 1074 WP_003194305.1 DNA integrity scanning diadenylate cyclase DisA -
  MA06_RS15395 (DJ92_2692) - 2569579..2570697 (+) 1119 WP_003194307.1 PIN/TRAM domain-containing protein -
  MA06_RS15400 (DJ92_2693) ispD 2570714..2571394 (+) 681 WP_006093092.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MA06_RS15405 (DJ92_2694) ispF 2571504..2571980 (+) 477 WP_018783076.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49917.48 Da        Isoelectric Point: 6.8376

>NTDB_id=121773 MA06_RS15385 WP_003194303.1 2566965..2568341(+) (radA) [Bacillus pseudomycoides strain 219298]
MAKKKTKFICQECGYQSPKYMGKCPGCGQWNTLVEEMEQVVSSRRINYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIDEMNPSFVVIDSIQTIYLPEVTSAPGSVSQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRAGLLLQNQDAYLKVAGGLRLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEVRRVSRIEQRVQEAAKLGFQRAIIPQKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=121773 MA06_RS15385 WP_003194303.1 2566965..2568341(+) (radA) [Bacillus pseudomycoides strain 219298]
ATGGCTAAAAAGAAAACGAAATTTATATGTCAAGAATGTGGTTATCAGTCACCAAAATATATGGGGAAGTGCCCTGGATG
TGGGCAATGGAATACGCTTGTCGAAGAGATGGAACAAGTTGTATCATCGCGTCGCATCAACTATGCAAATGCGATTCAAA
CGGAAGTAACAAAGCCAAGGCGTCTTACAGAAGTAGAAACAAAGTCAGAGGCACGGATTGAAACAAAATTTCAAGAATTT
AATCGTGTACTCGGTGGAGGAATTGTTGACGGTTCCCTCGTACTTATTGGAGGAGATCCTGGAATTGGAAAATCAACCTT
ACTACTACAAATATCATCGCAATTAGCAGATTCTTCGTATGATGTATTGTATATATCTGGTGAAGAATCAGCAAAACAAA
TTAAACTTCGTGCAGATCGTCTACATGTAAATGGAAGCAACTTATTTGTTGTTTCGGAAACAGATTTACAGCGAATTGCG
GCACATATCGATGAAATGAACCCTTCCTTTGTTGTCATTGATTCCATTCAAACTATTTATTTGCCAGAAGTAACCTCAGC
ACCAGGTAGTGTTTCGCAAGTACGTGAATGTACAGCGGAACTGATGAAACTTGCAAAAACGAAAGGAATTCCAATTTTTA
TCGTAGGACATGTAACGAAAGAAGGGGCAATTGCAGGACCGCGTATGTTAGAACATATGGTGGATGCGGTTCTTTATTTT
GAAGGAGACCGTCATCATACATATCGTATTTTGCGAGCGGTTAAAAATCGCTTTGGTTCTACAAATGAGATGGGTATTTT
TGAAATGAAGGAACTCGGTCTTGCGGAAGTATTAAATCCTTCTGAAATTTTTCTAGAAGAAAGACCTGTTGGAGTTGCTG
GCTCTACAGTTGTTGCTTCTATGGAAGGGACAAGGCCTGTTTTAGTAGAAATACAAGCATTAATTTCTCCTACTAGTTTT
GGAAATCCACGAAGAATGGCTACAGGGATTGATCATAATCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAGCAGG
TTTATTATTGCAAAACCAAGATGCGTATTTAAAGGTTGCTGGAGGATTGAGATTGGATGAACCAGCAATTGATTTGGCAG
TGGCTTTAAGTATTGCATCGAGTTTTCGAGATAAGTCAACTGCCCCGACCGATGCGGTAATAGGAGAAGTTGGGTTAACT
GGAGAAGTAAGAAGGGTATCGAGAATTGAACAACGAGTACAAGAAGCGGCTAAATTAGGATTTCAGCGTGCCATCATCCC
GCAAAAAAATTTAGGGGGTTGGACAATTCCTGAGGGGATTGAAGTAGTTGGTGTTTCTAATTTAGGAGAGGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

76.035

100

0.762
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627

Multiple sequence alignment