Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilJ   Type   Machinery gene
Locus tag   D082_RS08210 Genome accession   NZ_CP007542
Coordinates   1808654..1811266 (+) Length   870 a.a.
NCBI ID   WP_028948126.1    Uniprot ID   A0A068MYU9
Organism   Synechocystis sp. PCC 6714     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1803654..1816266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D082_RS08190 (D082_18250) - 1804094..1806004 (+) 1911 WP_028948130.1 long-chain fatty acid--CoA ligase -
  D082_RS08195 (D082_18260) - 1806229..1807392 (+) 1164 WP_028948129.1 response regulator -
  D082_RS08200 (D082_18280) pilH 1807592..1807957 (+) 366 WP_028948128.1 response regulator transcription factor Machinery gene
  D082_RS08205 (D082_18290) - 1808029..1808580 (+) 552 WP_028948127.1 chemotaxis protein CheW -
  D082_RS08210 (D082_18300) pilJ 1808654..1811266 (+) 2613 WP_028948126.1 methyl-accepting chemotaxis protein Machinery gene
  D082_RS08215 (D082_18310) - 1811279..1812070 (+) 792 WP_028948125.1 ABC transporter permease -
  D082_RS08220 (D082_18320) - 1812187..1812438 (+) 252 WP_028948124.1 TatA/E family twin arginine-targeting protein translocase -
  D082_RS08225 (D082_18340) - 1812779..1814176 (+) 1398 WP_028948123.1 sensor domain-containing diguanylate cyclase -

Sequence

Protein


Download         Length: 870 a.a.        Molecular weight: 93188.84 Da        Isoelectric Point: 4.0009

>NTDB_id=120735 D082_RS08210 WP_028948126.1 1808654..1811266(+) (pilJ) [Synechocystis sp. PCC 6714]
MATETNFSQLYGLAYAAYGQGDYQEASSHIEQLATDFPDDPNVLLLRGHIYVGLEQYALANEAYQAVIRLSDRQDLIDCA
HQALGQIQGNGTGDNGADDPFGQDQDWQNSLDNIDQGMDWDTGVFTEEDFGEPTLGRAPTPTNPGMDHPFGRSSGSFGTS
GASSYEIEHLDWSPEAFTDDLDSDDEQTMMPQTGESTFVVPPAFGEGVVDFADLAAQVPSAGMGAAAASEYPDYQSWDGG
DWSEGLGLDEPEVVPNVQTPTSGSYGGKELPSFDAFEEGVGDELSDFNLTDIAPELPDSSLFTQSTGLDLSSMGQAYDTQ
SGDEPVYGGANAAPPGMATWLKPEGATTGDLSSATSPFNDRPTSEVDAGFLGWFTNASLFKKQIYMAIASGLFSGVVVLV
LGNVVGLGGNPGDVPAPPGQTTEAPAQGAPTQGEVTPPSPEKEASKPSLLNLALLTALATVVGVFLINSLLMRQIKSIID
DLQNQFESIYEGNFNVRAKVRSEDELGALTQRFNYISQVILTATSEAQERAATTEKVREKLQRQVIRLLDDVEGASRGDL
TVQAEVTADELGAVADAFNLTIQNLREIVLQVKNAAKQVNNNCKDSESFARNNSSDALRMAEELAVTLNSVQLMTESIER
VAENAREAEEVAHTSSLTALKGGEAVERTVGGILQIRETVSETARKVKRLAEASQEISKIVAVISQIASRTNLLALNASI
QAARAGEAGRGFAIVADEVRQLADRSAKSLKEIEQIVLQIQSETGSVMTAMEEGIQQVIDVTDKSEQSKRALEDIIEVSN
RINTLVRSITADTVKQQENSGAVSNVMQSIELTAQETSQESQRVAGSLQTLVAISRDLLTSVERFRVESK

Nucleotide


Download         Length: 2613 bp        

>NTDB_id=120735 D082_RS08210 WP_028948126.1 1808654..1811266(+) (pilJ) [Synechocystis sp. PCC 6714]
ATGGCAACAGAAACCAATTTTTCGCAGCTTTATGGGTTGGCCTACGCTGCCTATGGACAGGGAGATTATCAAGAAGCATC
TTCCCACATTGAACAATTGGCCACTGATTTCCCCGATGATCCCAATGTTTTGTTGCTCCGGGGACATATCTATGTTGGCT
TGGAACAGTACGCCTTGGCCAACGAGGCTTACCAAGCTGTAATTCGACTTTCCGATCGCCAGGATTTAATTGATTGTGCC
CACCAGGCCCTGGGGCAAATCCAGGGGAATGGAACCGGGGACAATGGGGCCGATGACCCCTTTGGTCAGGATCAGGATTG
GCAAAACAGCCTAGATAACATCGATCAGGGCATGGATTGGGATACTGGGGTATTTACCGAGGAGGATTTTGGGGAACCAA
CCCTAGGCCGGGCCCCTACTCCCACCAATCCGGGCATGGATCATCCCTTTGGCCGTAGCTCTGGGTCCTTTGGTACCAGT
GGGGCAAGCAGCTATGAAATCGAACATTTAGATTGGAGTCCGGAGGCCTTCACCGACGATCTCGACTCGGACGATGAACA
AACCATGATGCCCCAAACAGGGGAATCTACCTTTGTGGTGCCACCGGCCTTTGGCGAAGGAGTGGTGGATTTTGCCGATT
TAGCGGCGCAAGTCCCCTCTGCGGGCATGGGAGCAGCGGCGGCTTCTGAATACCCGGATTACCAAAGTTGGGATGGAGGC
GATTGGAGCGAAGGCCTGGGCTTGGATGAGCCAGAAGTTGTTCCCAATGTGCAGACCCCCACTTCCGGGAGTTATGGTGG
CAAGGAATTACCTAGTTTTGATGCTTTCGAGGAAGGTGTTGGCGACGAGTTATCAGATTTTAACTTGACGGACATTGCTC
CGGAGTTGCCAGATTCTTCCCTATTTACCCAGAGCACCGGCCTGGATTTAAGCTCCATGGGCCAGGCCTATGACACCCAG
AGTGGTGATGAGCCAGTTTATGGAGGCGCTAATGCAGCACCGCCGGGCATGGCTACTTGGTTAAAACCGGAGGGGGCCAC
CACTGGAGATCTATCCAGTGCCACTAGTCCCTTTAATGACCGCCCCACCAGCGAAGTGGATGCTGGTTTTCTCGGTTGGT
TCACCAATGCTTCTCTCTTCAAGAAGCAAATTTATATGGCGATCGCCTCGGGACTGTTTTCCGGGGTGGTGGTTTTGGTA
TTGGGCAATGTGGTGGGTCTAGGGGGAAATCCTGGGGATGTGCCTGCGCCTCCGGGGCAAACCACAGAAGCTCCCGCACA
AGGGGCGCCGACCCAGGGGGAAGTAACACCTCCATCCCCTGAGAAAGAAGCAAGCAAACCTTCCCTATTGAATCTAGCCC
TCCTCACGGCTCTAGCCACGGTGGTCGGTGTCTTTTTAATCAACAGTCTCTTGATGCGACAGATTAAAAGTATTATCGAT
GATTTGCAAAATCAGTTTGAATCCATTTACGAAGGTAATTTCAACGTCCGGGCCAAGGTGCGATCGGAAGACGAATTGGG
AGCGTTGACCCAAAGGTTTAACTACATTTCTCAAGTTATTCTCACTGCCACCAGCGAAGCCCAGGAAAGGGCCGCCACCA
CCGAAAAAGTAAGGGAAAAATTACAACGACAGGTAATCCGCCTCCTAGATGACGTGGAGGGGGCCTCCCGGGGAGATTTG
ACTGTCCAGGCAGAGGTGACAGCGGACGAACTAGGGGCTGTGGCCGATGCCTTTAACCTGACTATCCAGAATCTGCGGGA
AATTGTCCTCCAGGTAAAAAATGCTGCCAAGCAGGTGAATAATAACTGTAAAGACAGTGAATCCTTTGCCCGCAATAATT
CCAGCGACGCCCTAAGGATGGCGGAGGAATTGGCCGTCACCCTCAACTCAGTTCAACTGATGACGGAATCCATTGAACGG
GTGGCAGAAAATGCGCGGGAAGCGGAGGAAGTAGCCCATACCTCATCGTTAACCGCATTGAAAGGTGGAGAGGCGGTGGA
AAGAACGGTGGGGGGAATTCTGCAAATTCGGGAAACGGTGTCGGAAACGGCCCGGAAAGTGAAACGGTTAGCGGAAGCGT
CCCAGGAAATTTCTAAGATTGTGGCGGTTATATCCCAAATTGCCTCCCGGACAAACTTGCTAGCCCTAAACGCTTCCATC
CAGGCAGCCCGGGCAGGGGAAGCGGGGCGGGGTTTTGCCATCGTGGCGGACGAAGTGCGGCAGTTGGCGGATCGTTCGGC
TAAATCCCTCAAGGAAATTGAACAGATCGTTTTGCAAATTCAAAGTGAGACCGGTTCCGTAATGACTGCCATGGAAGAAG
GCATTCAACAGGTAATTGATGTAACGGATAAGTCAGAACAGTCGAAACGAGCCCTGGAGGATATTATTGAGGTGTCTAAT
CGCATTAACACTTTGGTGCGTTCCATCACTGCGGATACAGTCAAACAACAGGAGAATTCCGGGGCGGTGTCCAACGTTAT
GCAGTCCATTGAACTGACGGCCCAGGAGACATCCCAGGAGTCCCAACGGGTGGCAGGATCTTTGCAAACCCTGGTGGCCA
TTTCCCGGGATCTGTTAACTTCGGTAGAACGGTTCCGGGTAGAAAGTAAGTAA

Domains

Predicted by InterproScan.

HAMP domain (HAMP)

(470-515)

HAMP domain (HAMP)

(556-587)

Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal)

(650-831)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A068MYU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilJ Synechocystis sp. PCC 6803

88.073

100

0.883

Multiple sequence alignment