Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   D082_RS05610 Genome accession   NZ_CP007542
Coordinates   1242482..1244500 (-) Length   672 a.a.
NCBI ID   WP_028948737.1    Uniprot ID   A0A068MXA9
Organism   Synechocystis sp. PCC 6714     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1237482..1249500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D082_RS05570 (D082_12450) thyX 1237600..1238250 (-) 651 WP_028948729.1 FAD-dependent thymidylate synthase -
  D082_RS05580 (D082_12470) - 1238482..1238619 (-) 138 WP_028948731.1 photosystem II reaction center protein K -
  D082_RS05585 (D082_12480) - 1238708..1239436 (-) 729 WP_028948732.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  D082_RS05590 (D082_12500) - 1239639..1239896 (+) 258 WP_028948733.1 BolA family protein -
  D082_RS05595 (D082_12520) grxD 1240090..1240413 (+) 324 WP_028948734.1 Grx4 family monothiol glutaredoxin -
  D082_RS18515 - 1240410..1240550 (-) 141 WP_158506493.1 hypothetical protein -
  D082_RS05600 (D082_12530) - 1240629..1240973 (+) 345 WP_028948735.1 YbaB/EbfC family nucleoid-associated protein -
  D082_RS05605 (D082_12540) hisD 1241012..1242316 (+) 1305 WP_028948736.1 histidinol dehydrogenase -
  D082_RS05610 (D082_12550) pilB/pilB1 1242482..1244500 (-) 2019 WP_028948737.1 GspE/PulE family protein Machinery gene
  D082_RS05615 (D082_12560) grpE 1244719..1245456 (+) 738 WP_028948738.1 nucleotide exchange factor GrpE -
  D082_RS05620 (D082_12580) dnaK 1245736..1247814 (+) 2079 WP_028948739.1 molecular chaperone DnaK -
  D082_RS05625 (D082_12590) - 1247862..1248896 (+) 1035 WP_028948740.1 AI-2E family transporter -

Sequence

Protein


Download         Length: 672 a.a.        Molecular weight: 75081.69 Da        Isoelectric Point: 5.2579

>NTDB_id=120728 D082_RS05610 WP_028948737.1 1242482..1244500(-) (pilB/pilB1) [Synechocystis sp. PCC 6714]
MTSSSSSKKSRAVALRNYFSPFGNKLVAGGHVEPEQLRQALVQVKKTGRSLPEEIKAVTGRELSPELLRQYKKNQLFELK
VLYGVDSVDPEVAPIATREMAELIGKFFPLDTCRRFKFLPLAQQDGEPPSVLVAMVDPDNLAAQDELNRILRVKGFELRR
LVVTQDDFNQLLDQYYAIREEWEAAQEKQDQEKALDKLNDLTDIVGSIDMNLGEVKDDTDSALDSNDANQAPVINLVNKI
LAKALQEGTSDIHVEPQEETLRIRFRKDGVLNQAFDPLPRKITPAVVARFKIMADMDIAERRSPQDGKIRRIYQGRKVDF
RVNTLPSRYGEKVCLRILDNTATQLGLDFLITNPETLQTVRELAGRPYGLMLVTGPTGSGKSTTLYSVLAERNSPEVNIN
TAEDPIEYALPGITQVQVIREKGMDFSNILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIAR
LDEMGVEPFMVSGSLLGVLAQRLMRKVCTECRIAYHPSKEELSRFGLSSSADDVVTFYKANTLSPDEIHLARQKGNLCQK
CSGTGYKGRVGVYEVMRNSERIAELINQGATTDRIKDCAVEEGMITLLAYSLNLVQEGYTTLEEVERVTFTDTGLESEMR
AKRKSALSCRVCSAELQQEWLDCPYCMTPRFS

Nucleotide


Download         Length: 2019 bp        

>NTDB_id=120728 D082_RS05610 WP_028948737.1 1242482..1244500(-) (pilB/pilB1) [Synechocystis sp. PCC 6714]
ATGACATCTTCTTCCTCTTCCAAGAAAAGCCGTGCCGTTGCCCTACGGAATTATTTTTCCCCCTTTGGTAACAAACTGGT
GGCGGGGGGCCATGTGGAACCGGAACAGTTACGACAGGCCCTAGTGCAGGTCAAAAAAACTGGACGATCCTTACCGGAGG
AGATCAAAGCAGTAACTGGCCGAGAATTATCCCCAGAACTACTGCGGCAGTACAAAAAGAATCAGTTGTTTGAGCTCAAA
GTGCTCTACGGGGTAGATAGCGTTGATCCAGAAGTAGCTCCGATCGCCACAAGGGAGATGGCGGAGTTAATTGGTAAATT
CTTCCCTCTGGATACTTGCCGGCGGTTTAAGTTTTTGCCCCTAGCCCAACAGGACGGAGAACCTCCCAGCGTGTTGGTGG
CCATGGTGGACCCCGATAACTTGGCTGCCCAGGACGAGCTGAACCGAATTTTGCGGGTCAAGGGCTTTGAGTTGAGACGT
TTGGTGGTTACCCAGGATGATTTTAACCAACTTCTAGACCAGTACTATGCCATTCGGGAAGAGTGGGAAGCGGCCCAGGA
AAAGCAAGACCAGGAAAAAGCCTTAGATAAACTCAATGACCTGACGGACATTGTCGGTTCCATCGACATGAATCTGGGGG
AAGTCAAGGACGATACAGATAGTGCTCTGGACTCCAATGATGCCAATCAAGCCCCGGTGATTAACCTGGTCAACAAGATT
TTGGCCAAGGCTCTCCAGGAAGGGACGTCCGACATTCACGTTGAACCCCAGGAGGAAACCCTAAGAATCCGTTTCCGCAA
GGATGGAGTTTTGAATCAAGCTTTTGATCCCCTGCCTCGGAAAATTACCCCGGCGGTGGTGGCCCGATTTAAAATTATGG
CGGATATGGACATTGCCGAGAGACGGTCTCCCCAGGATGGCAAAATTCGCCGTATTTACCAAGGCAGAAAGGTGGATTTT
CGGGTAAACACTTTGCCTAGCCGCTATGGGGAAAAGGTTTGTTTGCGGATTTTGGATAATACCGCCACCCAGTTGGGCTT
GGATTTCTTGATTACTAACCCTGAAACGTTACAAACAGTACGGGAATTGGCTGGCCGGCCCTATGGTTTGATGTTGGTGA
CGGGACCTACGGGGTCGGGGAAATCCACCACCCTCTACTCAGTCTTGGCGGAGCGCAATAGTCCCGAGGTGAATATCAAC
ACAGCGGAAGACCCCATTGAGTATGCTCTGCCCGGTATTACTCAGGTGCAGGTGATTCGGGAAAAAGGGATGGATTTTTC
CAATATTCTGCGGGCTTTTATGCGTCAGGATCCCGATGTGATTCTGGTGGGGGAAACCAGGGATAAGGAAACGGCTAAAA
CGGCCATTGAGGCAGCCCTAACTGGTCACTTAGTTTTAACTACCCTCCACACCAATGATGCCGCCGGGGCGATCGCCCGC
TTAGATGAAATGGGGGTGGAACCGTTTATGGTATCAGGGTCATTACTGGGGGTACTGGCCCAAAGGCTGATGCGGAAAGT
TTGCACTGAATGTCGCATTGCTTACCATCCTAGCAAGGAAGAATTATCCCGTTTTGGTTTATCCTCCTCCGCCGATGACG
TGGTTACATTCTACAAAGCCAATACCCTCAGCCCTGACGAAATCCATCTGGCTCGGCAAAAGGGCAATTTGTGTCAAAAA
TGCAGTGGCACCGGCTACAAAGGCCGGGTGGGAGTCTATGAAGTAATGCGTAATTCCGAGCGCATTGCCGAGTTAATTAA
CCAAGGAGCCACCACCGATCGCATCAAAGATTGTGCTGTGGAGGAAGGTATGATCACCCTCCTAGCTTACAGTCTTAATT
TAGTACAAGAGGGCTATACTACCCTGGAAGAGGTGGAACGGGTCACCTTCACCGACACCGGGCTGGAGTCGGAAATGCGG
GCCAAGCGTAAGAGCGCCCTATCCTGCCGGGTTTGCTCCGCAGAGCTACAACAGGAATGGCTTGATTGTCCCTATTGCAT
GACTCCACGCTTTAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(239-506)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(73-180)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A068MXA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

96.131

100

0.961

Multiple sequence alignment