Detailed information NTG0120127

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: UIB01_RS01395
• Genome accession: NZ_CP007509
• Coordinates: 294699..295844 (+)
• Length: 381 a.a.
• NCBI ID: WP_038656162.1
• Uniprot ID: -
• Organism: Stutzerimonas decontaminans strain 19SMN4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 289699..300844

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
UIB01_RS01375 (UIB01_01400)	-	291414..292004 (-)	591	WP_038656154.1	YggT family protein	-
UIB01_RS01380 (UIB01_01405)	proC	292014..292838 (-)	825	WP_038656156.1	pyrroline-5-carboxylate reductase	-
UIB01_RS01385 (UIB01_01410)	-	292849..293538 (-)	690	WP_038656158.1	YggS family pyridoxal phosphate-dependent enzyme	-
UIB01_RS01390 (UIB01_01415)	pilT	293605..294639 (+)	1035	WP_038656160.1	type IV pilus twitching motility protein PilT	Machinery gene
UIB01_RS01395 (UIB01_01420)	pilU	294699..295844 (+)	1146	WP_038656162.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
UIB01_RS01400 (UIB01_01425)	-	295841..296677 (-)	837	WP_038656164.1	TIGR03915 family putative DNA repair protein	-
UIB01_RS01405 (UIB01_01430)	-	296677..297888 (-)	1212	WP_038665304.1	putative DNA modification/repair radical SAM protein	-
UIB01_RS01410 (UIB01_01435)	-	298022..298417 (+)	396	WP_038656166.1	TM2 domain-containing protein	-
UIB01_RS01415 (UIB01_01440)	-	298494..299768 (-)	1275	WP_038656168.1	dihydroorotase	-
UIB01_RS01420 (UIB01_01445)	-	299768..300784 (-)	1017	WP_038656170.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42414.72 Da
• Isoelectric Point: 6.8857

>NTDB_id=120127 UIB01_RS01395 WP_038656162.1 294699..295844(+) (pilU) [Stutzerimonas decontaminans strain 19SMN4]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLASVSKGLSLEVSDEDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=120127 UIB01_RS01395 WP_038656162.1 294699..295844(+) (pilU) [Stutzerimonas decontaminans strain 19SMN4]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTATTCATCACGGCCGGCGTGCCGCCTTC
GATGAAGGTCAATGGCAAGATCATGCCGGTGAGCAAGACGGCGATGTCGCCGGAGATGACCCGGGAAACCGTGCATGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAAAACCATGAGTGCAATTTCGCCATCAGTGCCCGTGGTATTGGTCGC
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGCCGGGATGGTGCTGCGGCGCATCGAAACCAACATTCCGACCATCGA
AGAGCTGAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTCCTGTTCGTCGGCGCGACCGGTA
CCGGCAAGTCGACCTCGCTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAGGAT
CCAATCGAATTCATCCACCAGCATCAGAGCTGCATCGTCACCCAGCGTGAGGTCGGCATCGATACCAGCTCGTTCGAGGT
GGCACTGAAGAACACCCTGCGCCAGGCACCGGACGTGATTCTCATCGGCGAGATCCGTACTCGTGAAACCATGGACTACG
CCGTGGCCTTCGCCGAAACCGGTCACCTGTGTCTGGCGACGCTGCACGCCAACAACGCGAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGATCTTTCGCTCAACCTCAAGGCCATCGTCGCTCAGCA
GCTGGTGCCTACGCCTGATGGCAAGGGCCGTCGCGCGGTAATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGATCTGA
TCCGCAAGGGCGAGGTGCATGAACTCAAATCGTTGATGAAGCGCTCCACCGAACTGGGCATGCAGACCTTCGATCAGGCG
CTGTATAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCATTGCTGCACGCCGACTCGGCCAACGACCTGCGCCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAGCATCTGGCCAGCGTGTCCAAGGGCCTGAGCCTGGAAGTCAGTGACG
AGGACCCAGGCCGTAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.226	100	0.942
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388